Analysis of retinotectal projection and tectal layer formation

• Project/Area Number: 22570199
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tohoku University
• Principal Investigator: NAKAMURA Harukazu 東北大学, 大学院・生命科学研究科, 教授 (90079690)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 網膜視蓋投射 / 視蓋層形成 / ニワトリ胚 / エレクトロポレーション / Engrailed / 視蓋 / 層形成 / Tet-on / En2 / スライスカルチャー / 動画 / 層構築 / Er81

Research Abstract

In this study, we have shown that some part of the optic fibers run deep layers of the tectum from the first. These fibers are transient and disappear after birth. En2 is expressed in laminae g-j of SGFS in tecta of around E10. Misexpression of En2 showed that En2 expressing cells cannot stay in the superficial layers of the tectum. Time lapse analysis showed that En2 expressing cells went out of the superficial layer and settled in lamina i. The results indicate that En2 plays crucial roles in tectal layer formation. We found that layer IV, and V are composed of cells that have migrated tangentially, and that neuropilin1 is expressed in layer IV, V cells and sema3A is expressed in layer VI cells. Sema3A overexpression resulted in abnormal layer formation of the tectum. The results indicate that neuropilin-Semaphorin repulsive system plays crucial roles in tectal layer formation.

Research Products

• [Journal Article] Electroporation: Past, present and future (2013)
  • Author(s): Nakamura, H., Funahashi, J.
  • Journal Title: Dev Growth Differ Volume: 55 Pages: 15-19
  • DOI: 10.1007/978-1-60327-483-8_27
  • NAID: 40019549265
  • ISBN: 9781588294319, 9781603274838
  • Peer Reviewed
• [Journal Article] Coactosin accelerates cell dynamism by promoting actin polymerization (2013)
  • Author(s): Hou, X., Katahira, T., Ohashi, K., Mizuno, K., Sugiyama, S., Nakamura, H.
  • Journal Title: Dev Biol Volume: 379 Issue: 1 Pages: 53-63
  • DOI: 10.1016/j.ydbio.2013.04.006
  • Peer Reviewed
• [Journal Article] Electroporation: Past, present and future (2013)
  • Author(s): Harukazu Nakamura and Junichi Funahashi
  • Journal Title: Develop. Growth Differ Volume: 55 Issue: 1 Pages: 15-19
  • DOI: 10.1111/dgd.12012
  • NAID: 40019549265
  • Peer Reviewed
• [Journal Article] Optogenetic Probing and Manipulation of the Calyx-Type Presynaptic Terminal in the Embryonic Chick Ciliary Ganglion. (2013)
  • Author(s): Egawa, R., Hososhima, S., Hou, X., Katow, H, S., Ishizuka, T. Nakamura, H., and Yawo, H.
  • Journal Title: PLoS ONE Volume: 8 Issue: 3 Pages: e59179-e59179
  • DOI: 10.1371/journal.pone.0059179
  • Peer Reviewed
• [Journal Article] Nuclear translocation of intracellular domain of PRTG by proteolytic cleavage (2012)
  • Author(s): Watanabe, Y., Nakamura, H.
  • Journal Title: Dev. Growth Differ Volume: 54 Issue: 2 Pages: 167-176
  • DOI: 10.1111/j.1440-169x.2011.01315.x
  • Peer Reviewed
• [Journal Article] Nuclear translocation of FGF8 and its implication to induce Sprouty2 (2012)
  • Author(s): Suzuki, A., Harada, H. Nakamura, H.
  • Journal Title: Dev Growth Differ Volume: 54 (4) Issue: 4 Pages: 463-473
  • DOI: 10.1111/j.1440-169x.2012.01332.x
  • NAID: 10031136917
  • Peer Reviewed
• [Journal Article] Otx2 is involved in the regional specification of the developing retinal pigment epithelium by preventing the expression of sox2 and fgf8, factors that induce neural retina differentiation (2012)
  • Author(s): Daisuke Nishihara, Ichiro Yajima, Hiromasa Tabata, Masato Nakai, Nagaharu Tsukiji, Tatsuya Katahira, Kazuhisa Takeda, Shigeki Shibahara, Harukazu Nakamura, Hiroaki Yamamoto
  • Journal Title: PLoS One Volume: e48879 Issue: 11 Pages: e48879-e48879
  • DOI: 10.1371/journal.pone.0048879
  • Peer Reviewed
• [Journal Article] 脊椎動物の脳はいかにしてかたちづくられるか (2012)
  • Author(s): 仲村春和
  • Journal Title: 日仏生物学会誌 Volume: 52 Pages: 4-15
• [Journal Article] Genetically-defined lineage tracing of Nkx2.2-expressing cells in chick spinalcord (2011)
  • Author(s): Gotoh H, Ono K, Takebayashi H, Harada H, Nakamura H, Ikenaka
  • Journal Title: Dev. Biol Volume: 349 Issue: 2 Pages: 504-511
  • DOI: 10.1016/j.ydbio.2010.10.007
  • Peer Reviewed
• [Journal Article] Conditional knockdown of target gene expression by tetracycline regulated transcription of double strand RNA (2011)
  • Author(s): Hou, X., Omi, M., Harada, H., Ishii, S., Takahashi, Y., Nakamura, H.
  • Journal Title: Dev Growth Differ Volume: 53 Issue: 1 Pages: 69-75
  • DOI: 10.1111/j.1440-169x.2010.01229.x
  • NAID: 10027899337
  • Peer Reviewed
• [Journal Article] Protogenin mediates cell adhesion for ingression and re-epithelialization of the paraxial mesodermal cells (2011)
  • Author(s): Ito, K., Nakamura, H., Watanabe, Y.
  • Journal Title: Dev Biol Volume: 351 Issue: 1 Pages: 13-24
  • DOI: 10.1016/j.ydbio.2010.11.024
  • Peer Reviewed
• [Journal Article] Identification of retinotectal projection pathway in the deep tectal laminae in the chick (2011)
  • Author(s): Omi, M., Harada, H., Nakamura, H.
  • Journal Title: J Comp Neurol Volume: 519 Issue: 13 Pages: 2615-2621
  • DOI: 10.1002/cne.22642
  • Peer Reviewed
• [Journal Article] Identification of retinotectal projection pathway in the deep tectal laminae in the chick (2011)
  • Author(s): Omi M, Harada H, Nakamura H.
  • Journal Title: J Comp Neurol. Volume: 519 Pages: 2615-2621
  • Peer Reviewed
• [Journal Article] Genetically-defined lineage tracing of Nkx2.2-expressing cells in chick spinal cord (2011)
  • Author(s): Gotoh H, Ono K, Takebayashi H, Harada H, Nakamura H, Ikenaka
  • Journal Title: Dev.Biol. Volume: 349 Pages: 504-511
  • Peer Reviewed
• [Journal Article] Conditional knockdown of target gene expression by tetracycline regulated transcription of double strand RNA (2011)
  • Author(s): Hou, X., Omi, M., Harada, H., Ishii, S., Takahashi, Y., Nakamura, H.
  • Journal Title: Dev.Growth Differ Volume: 53 Pages: 69-75
  • NAID: 10027899337
  • Peer Reviewed
• [Journal Article] Activation of Ras-ERK pathway by Fgf8 and its downregulation by Sprouty2 for the isthmus organizing activity (2010)
  • Author(s): Suzuki-Hirano, A., Harada, H., Sato, T., Nakamura, H.
  • Journal Title: Dev. Biol Volume: 337 Issue: 2 Pages: 284-293
  • DOI: 10.1016/j.ydbio.2009.10.044
  • Peer Reviewed
• [Journal Article] Gene manipulation of chick embryos in vitro, EC culture, and long survival in transplanted eggs (2010)
  • Author(s): Tanaka, J., Harada, H., Ito, K., Ogura, T., Nakamura, H.
  • Journal Title: Dev Growth Differ Volume: 52 Issue: 7 Pages: 629-634
  • DOI: 10.1111/j.1440-169x.2010.01198.x
  • Peer Reviewed
• [Journal Article] Activation of Ras-ERK pathway by Fgf8 and its downregulation by Sprouty2 for the isthmus organizing activity (2010)
  • Author(s): Suzuki-Hirano, A., Harada, H., Sato, T., Nakamura, H.
  • Journal Title: Dev.Biol. Volume: 337 Pages: 284-293
  • Peer Reviewed
• [Journal Article] Gene manipulation of chick embryos in vitro, EC culture, and long survival in transplanted eggs (2010)
  • Author(s): Tanaka, J., Harada, H., Ito, K., Ogura, T., Nakamura, H.
  • Journal Title: Dev.Growth Differ. Volume: 52 Pages: 629-634
  • Peer Reviewed
• [Presentation] 脊椎動物脳の領域化のメカニズム (2012)
  • Author(s): 仲村春和
  • Organizer: 埼玉大学研究機構・脳科学融合研究センターシンポジウム「脳の発生・発達とその破綻の理解をめざして」
  • Place of Presentation: 埼玉大学
  • Year and Date: 2012-10-06
• [Presentation] Conditional knockdown of target gene expression: Tet-on of transcription of double strand RNA. Chick 7: Avian Model Systems (2012)
  • Author(s): Hou, X., Omi, M., Harada, H., Ishii, S., Takahashi, Y., Nakamura, H.
  • Organizer: 7th International Chick Meeting
  • Place of Presentation: Nagoya
• [Presentation] Time-lapse imaging system with shell-less culture chamber. Chick 7: Avian Model Systems (2012)
  • Author(s): Funahashi, J., Nakamura, H.
  • Organizer: 7th International Chick Meeting
  • Place of Presentation: Nagoya
• [Presentation] Chair of the Organizers (2012)
  • Author(s): Harukazu Nakamura
  • Organizer: Chick 7: Avian Model Systems, 7^<th> International Chick Meeting
  • Place of Presentation: Nagoya
• [Presentation] Conditional knockdown of target gene expression: Tet-on of transcription of double strand RNA. (2012)
  • Author(s): Hou, X., Omi, M., Harada, H., Ishii, S., Takahashi, Y., Nakamura, H.
  • Organizer: Chick 7: Avian Model Systems, 7th International Chick Meeting.
  • Place of Presentation: 名古屋
• [Presentation] Class 3 semaphorins regulate tectal laminar formation (2012)
  • Author(s): Watanabe, Y., Nakamura, H.
  • Organizer: Joint Meeting of JSDB 45th and JSCB 64th
  • Place of Presentation: 神戸
• [Presentation] Involvement of En2 in tectal laminar formation in chick embrhyos (2012)
  • Author(s): Omi, M., Watanabe, Y., Nakamura, H.
  • Organizer: Joint Meeting of JSDB 45th and JSCB 64th
  • Place of Presentation: 神戸
• [Presentation] 脊椎動物脳の領域化のメカニズム (2012)
  • Author(s): 仲村春和
  • Organizer: 埼玉大学研究機構・脳科学融合研究センターシンポジウム
  • Place of Presentation: 埼玉
• [Presentation] Class 3 semaphorins regulate tectal laminar formation (2012)
  • Author(s): Watanabe, Y., Nakamura, H.
  • Organizer: Joint Meeting of JSDB 45th and JSCB 64th
  • Place of Presentation: Kobe
• [Presentation] Ivolvement of En2 in tectal laminar formation in chick embrhyos (2012)
  • Author(s): Omi, M., Watanabe, Y., Nakamura, H.
  • Organizer: Joint Meeting of JSDB 45th and JSCB 64th
  • Place of Presentation: Kobe
• [Presentation] Possibility of FGF8 as a nuclear factor. Third UCL-Tohoku University Joint Symposium (2011)
  • Author(s): Suzuki A, Harada H, Nakamura H
  • Organizer: From Cell/Developmental Biology to Human Deseases. London
  • Place of Presentation: Great Britain
  • Year and Date: 2011-10-19
• [Presentation] From Cell/Developmental Biology to human diseases,Organizers (2011)
  • Author(s): Harukazu Nakamura & Shinichi Ohnuma
  • Organizer: UCL-Tohoku University Symposium
  • Place of Presentation: London, Great Britain
  • Year and Date: 2011-10-19
• [Presentation] アクチン結合タンパク質、コアクトシンの発達期ニワトリ胚毛様体神経節における局在-Localization of the actin binding protein, coactosin that expressed in the developing chick ciliary ganglion- (2011)
  • Author(s): 細島頌子, 侯旭濱, 石塚徹, 仲村春和, 八尾寛
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] 私たちの脳はいかにして作られ、機能するようになるか (2011)
  • Author(s): 仲村春和
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄(招待講演)
  • Year and Date: 2011-05-24
• [Presentation] Involvement of Pax6 in the retinal pigment epithelium development (2011)
  • Author(s): Daisuke Nishihara, Akiha Kawasaki-Nishihara, Nagahara Tsukiji, Harukazu Nakamura, Hiroaki Yamamoto
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-20
• [Presentation] Two modes of tangential cell migration in the developing chick optic tectum (2011)
  • Author(s): Yuji Watanabe, Harukazu Nakamura
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-19
• [Presentation] Novel retinal fiber pathway in the deep laminae of the developing chick optic tectum (2011)
  • Author(s): Minoru Omi, Hidekiyo Harada, Harukazu Nakamura
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-19
• [Presentation] Fgf8 may function as a nuclear factor (2011)
  • Author(s): Ayumu Suzuki, Hidekiyo Harada, Harukazu Nakamura
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-19
• [Presentation] Essential roll of SOX5 in the semicircular canal development of zebrafish (2011)
  • Author(s): Sympei Tanino, Gembu Abe, Koichi Kawakami, Harukazu Nakamura, Jun-ichi Funahashi
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-19
• [Presentation] Expression of the actin-binding proteins, coactosin and drebrin, in the developing chick ciliary ganglion (2011)
  • Author(s): Shoko Hososhima, Xubin Hou, Harukazu Nakamura, Tour Ishizuka, Hiromu Yawo
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-19
• [Presentation] Second UCL-Tohoku University Symposium (2011)
  • Author(s): Harukazu Nakamura& Shinichi Ohnuma
  • Organizer: From Cell/Developmental Biology to Neuroscience. Organizers
  • Place of Presentation: Sendai
  • Year and Date: 2011-01-23
• [Presentation] Novel retinotectal projection pathway in deeper laminae of the developing chick optic tectum (2011)
  • Author(s): Minoru Omi, Hidekiyo Harada, Harukazu Nakamura
  • Organizer: The 1st Tohoku International Symposium on Multidisciplinary Neuroscience
  • Place of Presentation: Sendai
  • Year and Date: 2011-01-21
• [Presentation] FGF8 localizes in the nucleus-The possibility of new function- (2011)
  • Author(s): Ayumu Suzuki, Hidekiyo Harada, Harukazu Nakamura
  • Organizer: The 1st Tohoku International Symposium on Multidisciplinary Neuroscience
  • Place of Presentation: Sendai
  • Year and Date: 2011-01-21
• [Presentation] SOX5 is required for semicicular canal development in Zebrafish (2010)
  • Author(s): Sympei Tanino, Gembu Abe, Koichi Kawakami, Harukazu Nakamura, Jun-ichi Funahashi
  • Organizer: BMB2010第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Involvement of Pea3/Ets transcription factor Erm in the neuronal axis formation by repressing Shh expression (2010)
  • Author(s): 原田英斉, 尾身実, 佐藤達也, 仲村春和
  • Organizer: BMB2010第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] ニワトリ胚網膜視蓋投射における新規軸索経路 (2010)
  • Author(s): 尾身実, 原田英斉, 仲村春和
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-25
• [Presentation] New retinotectal projection pathway in deeper laminae of the developing chick optic tectum (2010)
  • Author(s): Minoru Omi, Hidekiyo Harada, Harukazu Nakamura
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-22
• [Presentation] Involvement of Mitf in the retinal pigment epthelium development and its possible regulators (2010)
  • Author(s): Daisuke Nishihara, Nagahara Tsukiji, Ichiro Yajima, Kazuhisa Takeda, Shigeki Shibahara, Harukazu Nakamura, Hiroaki Yamamoto
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-22
• [Presentation] Visualization of tangential neuronal migration in the developing chick optic tectum (2010)
  • Author(s): Yuji Watanabe, Harukazu Nakamura
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-21
• [Presentation] Analysis of translocation of Fgf8 to the nuclei (2010)
  • Author(s): Ayumu Suzuki, Hidekiyo Harada, Harukazu Nakamura
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-21
• [Presentation] Organizing signal for development of the tectum and cerebellum. UCL-Tohoku University Symposium, From Cell/Developmental Biology to Neuroscience (2010)
  • Author(s): Harukazu Nakamura
  • Organizer: Organizers: Harukazu Nakamura & Shin-ichiOhnuma
  • Place of Presentation: London, Great Britain
  • Year and Date: 2010-03-15
• [Presentation] UCL-Tohoku University Symposium (2010)
  • Author(s): Harukazu Nakamura & Shinichi Ohnuma
  • Organizer: From Cell/Developmental Biology to Neuroscience. Organizers
  • Place of Presentation: London, Great Britain
  • Year and Date: 2010-03-15
• [Presentation] 脳の設計図とその書き換えによる自在な脳の作り替え (2009)
  • Author(s): 仲村春和
  • Organizer: 第2回東北大学バイオフォーラム～脳科学の革新的な発展を目指して～
  • Place of Presentation: 東京
  • Year and Date: 2009-06-05
• [Book] キメラ・クローン・遺伝子-生命の発生・進化をめぐる研究の歴史? (2012)
  • Author(s): 仲村春和 勝部憲一監訳
  • Publisher: 西村書店(Nicole Le Douarin著Des Chimeres,des Clones et des genesからの翻訳)
• [Book] 目的別で選べる遺伝子導入プロトコール ニワトリ胚への遺伝子強制発現およびノックダウン (2012)
  • Author(s): 仲村春和
  • Publisher: 実験医学別冊医学書院仲嶋一範,北村義浩,武内恒成/編
• [Book] 遺伝子導入プロトコール (2012)
  • Author(s): 仲村春和
  • Total Pages: 250
  • Publisher: ニワトリ胚への遺伝子強制発現およびノックダウン
• [Book] 目的別で選べる遺伝子導入プロトコール （ニワトリ胚への遺伝子強制発現およびノックダウンの章を担当） (2012)
  • Author(s): 仲村春和
  • Publisher: 医学書院
• [Book] Electroporation and Sonoporation in Developmental Biology. Ed (2009)
  • Author(s): Nakamura, H
  • Publisher: Springer Japan, Tokyo.
• [Book] Chapter 1 'Short history of electroporation for the study of developmental biology (2009)
  • Author(s): Harukazu Nakamura
  • Publisher: Springer Japan, Tokyo
• [Book] Chapter 2 'In ovo electroporation as a useful tool to pursue molecular mechanisms of neural development in chick embryos. Odani, N., Hou, X. (2009)
  • Author(s): Nakamura, H
  • Publisher: Springer Japan, Tokyo
• [Book] Chapter 10 'Retinal fiber tracing by in ovo electroporation (2009)
  • Author(s): Harada, H., Nakamura, H
  • Publisher: Springer Japan, Tokyo
• [Book] Chapter 12 'Clonal and widespread gene transfer by proviral electroporation for analysis of brain laminar formation (2009)
  • Author(s): Sugiyama, S., Nakamura, H
  • Publisher: Springer Japan, Tokyo