Analysis of mechanism for neural-epidermal boundary formation

• Project/Area Number: 22570200
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: MICHIUE Tatsuo 東京大学, 大学院・総合文化研究科, 准教授 (10282724)
• Co-Investigator(Kenkyū-buntansha): KURODA Hiroki 静岡大学, 大学院・教育学研究科, 准教授 (70402229)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 形態形成 / 胚パターニング / ツメガエル / プラコード / 境界規定 / 神経綉導 / アフリカツメガエル / パターニング / 神経誘導

Research Abstract

In this study, (1)We analyzed the function of PRDM12. When PRDM12 MO was injected, the expression of several placode formation was specifically inhibited. (2) We found that suitable dose of BMP/Wnt inhibitor efficiently induced placode. (3) The analysis of Xnr3 indicated that injection with Xnr3 effected on the shape of cells locatedin neural-epidermal border region, and that the expression of placode marker and brain marker was altered.

Research Products

• [Journal Article] Xnr3 affects brain patterning via cell migration in the neural-epidermal tissue boundary duringearly Xenopus embryogenesis. (2013)
  • Author(s): Morita M, Yamashita S, Matsukawa S, Haramoto Y, Takahashi S, Asashima M, Michiue T
  • Journal Title: Int J DevBiol
• [Journal Article] Hippo signaling components, Mst1 and Mst2, act as a switch between self-renewal and differentiation in Xenopus hemangioblast (2013)
  • Author(s): Nejigane S, Takahashi S, Haramoto Y, Michiue T, Asashima M
  • Journal Title: Int J Dev Biol
• [Journal Article] BMP signaling regulates the differentiation of mouse embryonic stem cells into lung epithelial cell lineages (2013)
  • Author(s): Ninomiya N, Michiue T, Asashima M, Kurisaki A
  • Journal Title: Vitro Cell Dev Biol Anim Volume: 49 Pages: 230-237
• [Journal Article] Characterization of CXC-type chemokine molecules in early Xenopus laevis development. (2013)
  • Author(s): Goto T, Michiue T, Ito Y, Asashima
  • Journal Title: Int J Dev Biol Volume: 57 Pages: 41-47
• [Journal Article] Xnr3 affects brain patterning via cell migration in the neural-epidermal tissue boundary during early Xenopus embryogenesis. (2013)
  • Author(s): Morita M
  • Journal Title: Int J Dev Biol. Volume: -
  • Peer Reviewed
• [Journal Article] BMP signaling regulates the differentiation of mouse embryonic stem cells into lung epithelial cell lineages. (2013)
  • Author(s): Ninomiya N, et al.
  • Journal Title: In Vitro Cell Dev Biol Anim Volume: 49 Issue: 3 Pages: 230-237
  • DOI: 10.1007/s11626-013-9589-1
  • Peer Reviewed
• [Journal Article] Hippo signaling components, Mst1 and Mst2, act as a switch between self-renewal and differentiation in Xenopus hemangioblast. (2013)
  • Author(s): Nejigane S, et al.
  • Journal Title: Int J Dev Biol. Volume: -
  • Peer Reviewed
• [Journal Article] Characterization of CXC-type chemokine molecules in early Xenopus laevis development. (2013)
  • Author(s): Goto T, et al.
  • Journal Title: Int J Dev Biol. Volume: 57 Issue: 1 Pages: 41-47
  • DOI: 10.1387/ijdb.120223ma
  • Peer Reviewed
• [Journal Article] mNanog possesses dorsal mesoderm-inducing ability by modulating both BMP and Activin/nodal signaling in Xenopus ectodermal cells (2012)
  • Author(s): Miyazaki A, Ishii K, Yamashita S, Nejigane S, Matsukawa S, Ito Y, Onuma Y, Asashima M, Michiue T
  • Journal Title: PLoS On Volume: 7
• [Journal Article] xCOUP-TF-B regulates xCyp26 transcription and modulates RA signaling for anterior neural patterning in Xenopus (2012)
  • Author(s): Tanibe M, Ishiura S, Asashima M, Michiue T
  • Journal Title: Int J Dev Biol Volume: 56 Pages: 239-244
• [Journal Article] mNanog possesses dorsal mesoderm-inducing ability by modulating bothBMP and Activin/nodal signaling in Xenopus ectodermal cells (2012)
  • Author(s): Aya Miyazaki
  • Journal Title: PLoS One Volume: 7巻 Issue: 10 Pages: e46630-e46630
  • DOI: 10.1371/journal.pone.0046630
  • Peer Reviewed
• [Journal Article] xCOUP-TF-B regulates xCyp26 transcription and modulates RA signaling for anterior neural patterning in Xenopus. (2012)
  • Author(s): Tanibe M, et al.
  • Journal Title: Int J Dev Biol. Volume: 56 Issue: 4 Pages: 239-244
  • DOI: 10.1387/ijdb.113482mt
  • Peer Reviewed
• [Journal Article] Rapamycin treatment causes developmental delay, pigmentation defects, and gastrointestinal malformation on Xenopus embryogenesis (2011)
  • Author(s): Moriyama Y, Ohata Y, Mori S, Matsukawa S, Michiue T, Asashima M, Kuroda H
  • Journal Title: Biochem Biophys Res Commun Volume: 404 Pages: 974-8
• [Journal Article] elopmental delay, pigmentation defects, and gastrointestinal malforma (2011)
  • Author(s): Moriyama, et al.
  • Journal Title: Biochem Biophys Res Commun. Volume: 404 Pages: 974-978
  • Peer Reviewed
• [Journal Article] Reduction of N-Glycolylneuraminic Acid in Human Induced Pluripotent Stem Cells Generated or Cultured under Feeder- and Serum-Free Defined Conditions (2010)
  • Author(s): Hayashi Y, Chan T, Warashina M, Fukuda M, Ariizumi T, Okabayashi K, Takayama N, Otsu M, Eto K, Furue MK, Michiue T, OhnumaK, Nakauchi H, Asashima M
  • Journal Title: PLoS One Volume: 5
• [Journal Article] Induction of neural crest cells from mouse embryonic stem cells in a serum-free monolayer culture (2010)
  • Author(s): Aihara Y, Hayashi Y, Hirata M, Ariki N, Shibata S, Nagoshi N, Nakanishi M, Ohnuma K, Warashina M, Michiue T, Uchiyama H, Okano H, Asashima M, Furue MK
  • Journal Title: Int J Dev Biol Volume: 54 Pages: 1287-94
• [Journal Article] Claudin5 genes encoding tight junction proteins are required for Xenopus heart formation (2010)
  • Author(s): Yamagishi M, Ito Y, Ariizumi T, Komazaki S, Danno H, Michiue T, AsashimaM
  • Journal Title: Dev. Growth Differ Volume: 52 Pages: 665-675
  • NAID: 10027566245
• [Journal Article] Claudin5 genes encoding tight junction proteins are required for Xenopus heart formation. (2010)
  • Author(s): Yamagishi, et al.
  • Journal Title: Dev.Growth Differ. Volume: 52 Pages: 665-675
  • Peer Reviewed
• [Presentation] mNanog possesses dorsal mesoderm-inducing ability by modulating both BMP and Activin/nodal signaling in Xenopus ectodermal cells (2012)
  • Author(s): Miyazaki A, Ishii K, Yamashita S,Nejigane S, Matsukawa S, Ito Y, Onuma Y, Asashima M, Michiue T.
  • Organizer: 14th international Xenopus conference
  • Place of Presentation: Hyeres, France
• [Presentation] (2012)
  • Author(s): Nejigane S, Michiue T, Asashima M.
  • Organizer: 14th international Xenopus conference
  • Place of Presentation: Hyeres, France
• [Presentation] Induction of pancreatic cells from human iPS cells in a serum-free monolayer condition (2012)
  • Author(s): Michiue T, Terada R, Ninomiya H, Takahashi S, Ohnuma K, Kusuda-Furue M, Miyajima A, Asashima M.
  • Organizer: 10th International Society for Stem Cell Research annual meeting
  • Place of Presentation: Yokohama, Japan
• [Presentation] Analysis of regulatory factors during early neural crest cell differentiation from mouse embryonic stem cells (2012)
  • Author(s): Aihara Y, Ito Y, Kurisaki A, Hayashi Y,Nejigane S, Uchiyama H, Michiue T, Furue-Kusuda M, Asashima M.
  • Organizer: 10th International Society for Stem Cell Research annual meeting
  • Place of Presentation: Yokohama, Japan
• [Presentation] mNanog possesses dorsal mesoderm-inducing ability by modulating both BMP and Activin/nodal signaling in Xenopus ectodermal cells. (2012)
  • Author(s): Michiue T
  • Organizer: 14th international Xenopus conference
  • Place of Presentation: イエール（フランス）
• [Presentation] Hippo signal components, Mst1 and Mst2,are involved in primitive hematopoiesis, myelopoiesis and vascular formation in Xenopus tropicalis blood island. (2012)
  • Author(s): Nejigane S
  • Organizer: 14th international Xenopus conference
  • Place of Presentation: イエール（フランス）
• [Presentation] ツメガエル胚の発生初期に於いてXnr3が神経パターニングに及ぼす影響 (2011)
  • Author(s): 森田真梨子、道上達男
  • Organizer: 第5回日本ツメガエル研究集会
  • Place of Presentation: 熱海
  • Year and Date: 2011-10-05
• [Presentation] Xneopus初期胚の頭部形成におけるレチノイン酸シグナリング調節因子の解析 (2011)
  • Author(s): 道上達男、谷邉美早紀
  • Organizer: 第5回日本ツメガエル研究集会
  • Place of Presentation: 熱海
  • Year and Date: 2011-10-05
• [Presentation] ツメガエル胚において中胚葉誘導反応能を調整する因子の同定 (2011)
  • Author(s): 宮崎綾, ら
  • Organizer: 第82回日本動物学会
  • Place of Presentation: 北海道旭川市
  • Year and Date: 2011-09-22
• [Presentation] ツメガエル初期胚の原腸陥入におけるxRhoGEF3の役割 (2011)
  • Author(s): 道上達男, ら
  • Organizer: 第82回日本動物学会
  • Place of Presentation: 北海道旭川市
  • Year and Date: 2011-09-21
• [Presentation] ツメガエル胚において中胚葉誘導反応能を調整する因子の同定 (2011)
  • Author(s): 宮崎綾ら
  • Organizer: 第82回日本動物学会
  • Place of Presentation: 旭川,北海道
  • Year and Date: 2011-09-05
• [Presentation] The role of Xnr3 in the nerual patterning in Xenopus (2011)
  • Author(s): 森田真梨子, ら
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄県宜野湾市
  • Year and Date: 2011-05-19
• [Presentation] TheroleofXnr3intheneuralpatterninginXenopus (2011)
  • Author(s): 森田真梨子ら
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 宜野湾,沖縄
  • Year and Date: 2011-05-05
• [Presentation] Establishment of an efficient neural crest cell induction system from mouse embryonic stem cells in defined medium (2011)
  • Author(s): Aihara Y, Hayashi Y, Hirata M, Ariki N, Shibata S, Nagoshi N, Nakanishi M, Ohnuma K, Warashina M, Michiue T, Uchiyama H, Okano H, Asashima M, Kusuda-Furue M.
  • Organizer: 9th International Society for StemCell Research annual meeting
• [Presentation] ツメガエル初期胚の原腸陥入におけるXRhoGEF3の役割 (2011)
  • Organizer: 第82回日本動物学会
  • Place of Presentation: 旭川,北海道
• [Presentation] ツメガエルの初期神経パターニングにおけるXnr3の役割 (2010)
  • Author(s): 森田真梨子, ら
  • Organizer: 第4回 ツメガエル研究集会関東支部会
  • Place of Presentation: 学習院大(東京)
  • Year and Date: 2010-07-24
• [Presentation] ツメガエルの初期神経パターニングにおけるXnr3の役割 (2010)
  • Author(s): 森田真梨子ら
  • Organizer: 第四回ツメガエル研究会関東支部会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-05
• [Book] キャンベル生物学原書第9版 (2013)
  • Publisher: 丸善出版
• [Book] 理系総合のための生命科学 (2013)
  • Publisher: 羊土社
• [Book] イラストで徹底理解するシグナル伝達キーワード事典 (2012)
  • Author(s): 山本雅、仙波憲太郎、山梨裕司
  • Publisher: 羊土社
• [Book] Mechanobiology During Vertebrate Organ Development (2011)
  • Author(s): Asashima M, Michiue T, Ohnuma K, Nakajima Y, Ito Y.
  • Publisher: Mechanosensing Biology
• [Book] 生命系逆引き研究室案内 (2010)
  • Author(s): 小宮山宏
  • Publisher: 東京図書