Do HOX genes in the visceral mesoderm control regional differentiationof the endoderm?

• Project/Area Number: 22570205
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Yamaguchi University
• Principal Investigator: MURAKAMI Ryutaro 山口大学, 大学院・医学系研究科, 教授 (40182109)
• Co-Investigator(Renkei-kenkyūsha): HARADA Yumiko 山口大学, 大学院・理工学研究科, 助教 (70284239)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 器官形成 / ショウジョウバエ / 中腸 / HOX / 内胚葉 / 内臓筋 / 部域分化 / HOX遺伝子 / Dpp

Research Abstract

This research project was undertaken in order to elucidate roles of HOX genes in the visceral mesoderm of Drosophila midgut. During the research period
epithelial genes suitable as specific markers for regional differentiation of the midgut were identified
and phenotypes of the mutants and misexpression of HOX genes were analyzed.
It was demonstrated that roles of HOX genes in the visceral mesoderm are induction and regulation of regional differentiation of the underlying endoderm.

Research Products

• [Journal Article] Dorsoventral patterning of the Drosophilahindgut is determined by interaction of genes under the control of two independent gene regulatory systems, the dorsal and terminal systems (2012)
  • Author(s): Takashi Hamaguchi, Shigeo Takashima, Aiko Okamoto, Misa Imaoka, Takashi Okumura, Ryutaro Murakami
  • Journal Title: Mechanisms of Development Volume: 129 Pages: 236-243
  • URL: http://dx.doi.org/10.1016/j.mod.2012.07.006
  • Peer Reviewed
• [Journal Article] Dorsoventral patterning of the Drosophila hindgut is determined by interaction of genes (2012)
  • Author(s): Takashi Hamaguchi 他（Takashi Hamaguchi）
  • Journal Title: Mechanisms of Development Volume: 129 Issue: 9-12 Pages: 236-243
  • DOI: 10.1016/j.mod.2012.07.006
  • Peer Reviewed
• [Journal Article] The drumstickgene acts cell-non-autonomously and triggers specification of the small intestine in the Drosophilahindgut (2011)
  • Author(s): Sarder N. Uddin, Masahiro Yano, Ryutaro Murakami
  • Journal Title: nternational Journal of Developmental Biology Volume: 55 Issue: 10-11-12 Pages: 945-952
  • DOI: 10.1387/ijdb.113343su
  • Peer Reviewed
• [Presentation] Ubx in the visceral mesoderm of Drosophila midgut specifies developmental fate of the underneath endoderm by suppressing the fate of adjacent midgut regions through the action of Dpp, Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists (2012)
  • Author(s): Masahiko Arishige
  • Organizer: The 64th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: Kobe International Conferece Center, Kobe
  • Year and Date: 2012-05-31
• [Presentation] ショウジョウバエ中腸内臓筋で発現するHOX遺伝子 Antp は内胚葉の部域分化を制御する (2012)
  • Author(s): 村上柳太郎（村上柳太郎）
  • Organizer: 中国四国地区生物系三学会合同大会島根大会
  • Place of Presentation: 島根大学(松江）
• [Presentation] Ubx in the visceral mesoderm of Drosophila midgut specifies developmental fate of the underneath endoderm by suppressing the fate of adjacent midgut regions through the action of Dpp (2012)
  • Author(s): Masahiko Arishige（Ryutaro Murakami)
  • Organizer: Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: Kobe International Conferece Center (Kobe)
• [Presentation] The HOX gene Antp in the midgut visceral mesoderm regulates both morphogenesis and cell differentiation on the endoderm. (2012)
  • Author(s): Keita Fujimoto (Ryutaro Murakami)
  • Organizer: Japanese Drosophila Research Conference 10
  • Place of Presentation: 慈恵医科大学（東京）
• [Presentation] ショウジョウバエ中腸上皮の部域特異的細胞分化における内臓筋の役割 (2012)
  • Author(s): 吉村 裕一（村上柳太郎）
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡）
• [Presentation] HOX genes in the visceral mesoderm of Drosophilamidgut regulate regionalizaion through the action od Dpp (2011)
  • Author(s): Keita Fujimoto
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜ポートアイランド、神戸
  • Year and Date: 2011-12-16
• [Presentation] Functional analysis of the Drosophila adenylate kinase-2 (Dak2) (2011)
  • Author(s): Miyuki Fuka, Masayuki Anno, Taigo Horiguchi, Koichi Fujisawa, Takafumi Noma, Ryutaro Murakami
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-14
• [Presentation] HOX genes in the visceral mesoderm of Drosophila midgut regulate regionalizaion through the action od Dpp (2011)
  • Author(s): Keita Fujimoto, Masahiko Arishige, Yumiko Harada, Ryutaro Murakami
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-13
• [Presentation] Function of the Drosphila T-box gene brachyenteron in development of the hindgut (2011)
  • Author(s): Masahiro Yano, Sarder Nasir Uddin, Yumiko Harada, Ryutaro Murakami
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-13
• [Presentation] Role of orthopedia, a Drosophila homeobox gene, in development of the hindgut (2011)
  • Author(s): Aiko Okamoto, Satoru Tome, Masahiro Yano, Yuri Hashimoto, Ryutaro Murakami
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-13
• [Presentation] Region-specific gene expression of the Drosophila midgut epithelium in the presence and absence of the visceral mesoderm (2011)
  • Author(s): Yuichi Yoshimura, Yumiko Harada, Keita Fujimoto, Masahiro Arishige, Ryutaro Murakami
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-13
• [Presentation] Ubxand abd-Ain the Visceral Mesoderm of the Midgut of DrosophilaRegulate Regional Differentiation of the Adjacent Endoderm through the Action of Dpp (2011)
  • Author(s): Yumiko Harada
  • Organizer: 1st Asia-Pacific Drosophila Research Conference
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2011-05-25
• [Presentation] drumstick, an Odd family gene, acts cell non-autonomously to specify the anterior-Most domain of the hindgut in Drosophila (2011)
  • Author(s): Sarder N.Uddin, Masahiko Yano, Ryutaro Murakami
  • Organizer: 1st Asia-Pacific Drosophila Research Conference
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Ubx and abd-A in the visceral mesoderm of the midgut of Drosophila regulate regional differentiation of the adjacent endoderm through the action of Dpp (2011)
  • Author(s): Yumiko Harada, Masahiko Arishige, Lily Shimooka, Ryutaro Murakami
  • Organizer: 1st Asia-Pacific Drosophila Research Conference
  • Place of Presentation: Taipei, Taiwan
• [Presentation] HOX genes in the visceral mesoderm of Drosophilamidgut regulate regionalizaion through the action od Dpp (2010)
  • Author(s): Keita Fujimoto
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド、神戸
  • Year and Date: 2010-12-10
• [Presentation] ショウジョウバエ中腸内臓筋で発現する HOX 遺伝子による内胚葉部域分化の制御 (2010)
  • Author(s): 原田由美子 他(村上柳太郎)
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド、神戸
  • Year and Date: 2010-12-10
• [Presentation] ショウジョウバエ中腸内臓筋で発現するHOX遺伝子による内胚葉部域分化の制御 (2010)
  • Author(s): 原田由美子, 他
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09