Studies on the localization of the signaling moleculeWnt and it's function in the cell

• Project/Area Number: 22570206
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kagoshima University
• Principal Investigator: SAKAI Masao 鹿児島大学, 大学院・理工学研究科, 教授 (40162268)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: アフリカツメガエル / Wnt / 小胞体 / lineage tracer / KDEL / chordin / シグナリング / シグナル伝達 / 細胞自律的発現 / in situ hybridization / n-β-galactosidase / chordin mRNA / PDI

Research Abstract

The aim of the present study is to show that Xwnt8, which has been shown to substitute the dorsal determinant in Xenopus early development, can act within the cell which inherit it. This study was carried out using morphological, immunohistochemical, lineage tracing and in situ hybridization techniques. We have shown, for the first time, that the signal transduction can occur within the cell, in a cell autonomous manner.

Research Products

• [Journal Article] Analysis of Head-Defects Caused by Hypergravity in Early Xenopus Embryos (2013)
  • Author(s): Makoto Yanagisawa, Keiko Kashiwagi, Hideki Hanada, Tadashi Shinkai, Satoshi Yoshitome, Hideo Kubo, Masao Sakai, Hirotada Fujii, Masamichi YamashitaAkihiko Kashiwagi, Nobuaki Furuno, Minoru Watanabe
  • Journal Title: Biological Sciences in Space Volume: 26 Pages: 1-6
  • NAID: 130004450619
  • Peer Reviewed
• [Journal Article] High cell-autonomy of the anterior endomesoderm viewed in blastomere fate shift during regulative development in the isolated right halves of four-cell stage Xenopus embryos (2012)
  • Author(s): Masaaki Koga, Takuro Nakashima, Shintaro Matsuo, Ryu Takeya, Hideki Sumimoto, Masao Sakai, Hiroshi Kageura
  • Journal Title: Development Growth and Differentiation Volume: 7 Pages: 717-729
  • NAID: 10031136937
  • Peer Reviewed
• [Journal Article] High cell-autonomy of the anterior endomesoderm viewed in blastomere fate shift during regulative development in the isolated right halves of four-cell stage Xenopus embryos (2012)
  • Author(s): Masaaki Koga
  • Journal Title: Development Growth and Differentiation Volume: 54 Issue: 7 Pages: 717-729
  • DOI: 10.1111/j.1440-169x.2012.01372.x
  • Peer Reviewed
• [Journal Article] Analysis of Head-Defects Caused by Hypergravity in Early <i>Xenopus</i> Embryos (2012)
  • Author(s): Makoto Yanagisawa
  • Journal Title: Biological Sciences in Space Volume: 26 Issue: 0 Pages: 1-6
  • DOI: 10.2187/bss.26.1
  • NAID: 130004450619
  • ISSN: 0914-9201, 1349-967X
  • Peer Reviewed
• [Presentation] Is chordin expressed only in the cells that received Xwnt8 mRNA (2012)
  • Author(s): Masao Sakai, Eriko Motomura, Tomohiro Narita, Shin-ichiro Nishimatsu, Tsutomu Nohno
  • Organizer: 14th International Xenopus Conference
  • Place of Presentation: フランス
  • Year and Date: 2012-09-12
• [Presentation] Importance of Wnt8 lipid modification during formation of the Spemann's organizer in early Xenopus embryos (2012)
  • Author(s): Tomohiro Narita
  • Organizer: Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: Kobe International Conference Center
• [Presentation] Is chordin expressed only in the cells that received Xwnt8 mRNA? (2012)
  • Author(s): Masao Sakai
  • Organizer: 14th International Xenopus Conference
  • Place of Presentation: Giens Peninsula, France
• [Presentation] Isolated ectoderm is fated to die in early Xenopus embryos (2011)
  • Author(s): Hirotaka Kato, Ayumi Horai, Eriko Motomura, Masao Sakai
  • Organizer: 44th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-20
• [Presentation] Expression of chordin in inverted embryos of Xenopus (2011)
  • Author(s): Eriko Motomura, Tomohiro Narita, Shin-ichiro Nishimatsu, Susumu Nohno Masao Sakai
  • Organizer: 44th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-20
• [Presentation] Expression of chordin in inverted embryos of Xenopus (2011)
  • Author(s): Eriko Motomural, Tomohiro Narita, Shin-ichiro Nishimatsu, Tsutomu Nhono, Masao Sakai
  • Organizer: 44th Annual Meeting for the Japanese Society of Developmental Biologists (cosponsor : the Asia-Pacific Developmental Biology Network)
  • Place of Presentation: Okinawa Convention Center (Okinawa)
• [Presentation] Isolated ectoderm is fated to die in early Xenopus embryos (2011)
  • Author(s): Hirotaka Kato, Eriko Motomura, Ippei Tanaka, Yusuke Higashi, Ayumi Hourai, Masao Sakai
  • Organizer: 44th Annual Meeting for the Japanese Society of Developmental Biologists (cosponsor : the Asia-Pacific Developmental Biology Network)
  • Place of Presentation: Okinawa Convention Center (Okinawa)
• [Presentation] Lonesome death of ectoderm (2010)
  • Author(s): Masao Sakai, Ayumi Horai, Hirotaka Kato, Yusuke Higashi, Eriko Motomura
  • Organizer: 13th International Xenopus Conference
  • Place of Presentation: カナダ
  • Year and Date: 2010-09-14
• [Presentation] Dorsalization by Wnt signaling occurs cell-autonomously in early Xenopus embryo (2010)
  • Author(s): Eriko Motomura, Narita Tomohiro, Shin-ichiro Nishimatsu, Tsutomu Nohno, Masao Sakai
  • Organizer: 13th International Xenopus Conference
  • Place of Presentation: カナダ
  • Year and Date: 2010-09-14
• [Presentation] Cell-autonomous expression of chordin caused by the injection of Xwnt8 mRNA in early Xenopus embryos (2010)
  • Author(s): Eriko Motomura, Tomohiro Narita, Shin-ichiro Nishimatsu, Tsutomu Nohno, Masao Sakai
  • Organizer: 43^<rd> Annual Meeting for the Japanese Society of Developmental Biologists Jointly Sponsored by the Asia-Pacific Developmental Biology Network
  • Place of Presentation: Kyoto International Conference Center, Kyoto City
• [Presentation] Dorsalization by Wnt signaling occurs cell-autonomously in early Xenopus embryo (2010)
  • Author(s): Eriko Motomura, Narita Tomohiro, Shin-ichiro Nishimatsu, Tsutomu Nohno, Masao Sakai
  • Organizer: 13th International Xenopus Conference
  • Place of Presentation: Lake Louise, Alberta, Canada
• [Presentation] Lonesome death of ectoderm (2010)
  • Author(s): Masao Sakai, Ayumi Horai, Hirotaka Kato, Yusuke Higashi, Eriko Motomura
  • Organizer: 13th International Xenopus Conference
  • Place of Presentation: Lake Louise, Alberta, Canada
• [Presentation] ツメガエル初期胚においてXwnt8は細胞自律的に働く:Xwnt8の細胞内蓄積と分泌 (2010)
  • Author(s): 成田知弘, 本村恵理子, 西松伸一郎, 坂井雅夫, 濃野勉
  • Organizer: (社)日本動物学会第81回東京大会
  • Place of Presentation: 東京都目黒区、東京大学
• [Presentation] ゼノパス初期胚でWntシグナリングによる背側化が細胞自律的に起こっている証拠 (2010)
  • Author(s): 本村恵理子, 成田知弘, 西松伸一郎, 坂井雅夫
  • Organizer: (社)日本動物学会第81回東京大会
  • Place of Presentation: 東京都目黒区、東京大学
• [Remarks]
  • URL: http://www.sci.kagoshima-u.ac.jp/~garu/
• [Remarks]
  • URL: http://frog.sci.kagoshima-u.ac.jp/Sakai-j.html