Basic developmental mechanisms involved in the ascidian development: its conservation and plasticity

• Project/Area Number: 22570207
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: SAIGA Hidetoshi 首都大学東京, 理工学研究科, 生命科学専攻 (60131918)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 進化発生 / 発生遺伝子 / ホヤ / Hox遺伝子 / 転写調節機構 / 保存性 / 可塑性 / マボヤ / Hox / クラスター / カタユウレイボヤ / Otx / Pitx / 付着器官 / 左右性

Research Abstract

In the present research, I have analyzed the structure, expression, function and transcriptional regulation in the development of two phylogenetically remote asicidians, Ciona intestinalis and Halocynthia roretzi. The analyses have shown that some aspects are well conserved and others are not between the two ascidian species. These results should provide important clues to further understand basic developmental features for ascidians and chordates.

Research Products

• [Journal Article] Transcription regulatory mechanism of Pitx in the papilla-forming region in the ascidian, Halocynthia roretzi, implies conserved involvement of Otx as the upstream gene in the adhesive organ development of chordates. (2012)
  • Author(s): Yoshida, K., Ueno, M., Niwano, T. and Saiga, H
  • Journal Title: Dev. Growth & Differ Volume: 54 Pages: 649-659
  • NAID: 10031136932
  • Peer Reviewed
• [Journal Article] Identical genomic organization of two hemichordate Hox clusters. (2012)
  • Author(s): Freeman, R.F., Ikuta, T., Wu, M., Koyanagi, R., Kawashima, T., Tagawa, K., Humphreys, Fang, G.-C., Fujiyama, A., Saiga, H., Lowe, C., Worley, K., Kirschner, M., Rokshar, D., Satoh, N. and Gerhart, J
  • Journal Title: Curr. Biol. Volume: 22 Pages: 2053-2058
  • Peer Reviewed
• [Journal Article] Transcription regulatory mechanism of Pitx in the papilla-forming region in the ascidian, Halocynthia roretzi, implies conserved involvement of Otx as the upstream gene in the adhesive organ development of chordates. (2012)
  • Author(s): Yoshida K, Ueno M, Niwano T, Saiga H.
  • Journal Title: Development Growth & Differentiation Volume: 54 Issue: 6 Pages: 649-659
  • DOI: 10.1111/j.1440-169x.2012.01366.x
  • NAID: 10031136932
  • Peer Reviewed
• [Journal Article] Identical genomic organization of two hemichordate hox clusters. (2012)
  • Author(s): Freeman R, Ikuta T, Wu M, Koyanagi R, Kawashima T, Tagawa K, Humphreys T, Fang GC, Fujiyama A, Saiga H, Lowe C, Worley K, Jenkins J, Schmutz J, Kirschner M, Rokhsar D, Satoh N, Gerhart J.
  • Journal Title: Current Biology Volume: 22 Issue: 21 Pages: 2053-2058
  • DOI: 10.1016/j.cub.2012.08.052
  • Peer Reviewed
• [Journal Article] Repression of Rx gene on the left side of the sensory vesicle by Nodal signaling is crucial for right-sided formation of the ocellus photoreceptor in the development of Ciona intestinalis (2011)
  • Author(s): Yoshida, K. and Saiga, H
  • Journal Title: Dev. Biol Volume: 354 Pages: 144-150
  • Peer Reviewed
• [Journal Article] Repression of Rx gene on the left side of the sensory vesicle by Nodal signaling is crucial for right-sided formation of the ocellus photoreceptor in the development of Ciona intestinalis (2011)
  • Author(s): Yoshida, K., Saiga, H.
  • Journal Title: Developmental Biology Volume: 354 Issue: 1 Pages: 144-150
  • DOI: 10.1016/j.ydbio.2011.03.006
  • Peer Reviewed
• [Journal Article] Repression of Rx gene on the left side of the sensory vesicle by Nodal signaling is crucial for right-sided formation of the ocellus photoreceptor in the development of Ciona intestinalis. (2011)
  • Author(s): Yoshida, K., Saiga, H.
  • Journal Title: Developmental Biology
  • Peer Reviewed
• [Journal Article] Spatial and temporal expression of two transcriptional isoforms of Lhx3, a LIM class homeobox gene, during embryogenesis of two phylogenetically remote ascidians, Halocynthia roretzi and Ciona intestinalis (2010)
  • Author(s): Kobayashi, M., Takatori, N., Nakajima, Y., Kumano, G., Nishida, H. and Saiga, H
  • Journal Title: Gene Exp. Patterns Volume: 10 Pages: 98-107
  • Peer Reviewed
• [Journal Article] Limited functions of Hox genes in the larval development of the ascidian (2010)
  • Author(s): Ikuta, T., Satoh, N. and Saiga, H
  • Journal Title: Ciona intestinalis. Development Volume: 137 Pages: 1505-1513
  • Peer Reviewed
• [Journal Article] Segregation of germ layer fates by nuclear migration-dependent localization of Not mRNA. (2010)
  • Author(s): Takatori, N., Kumano, G., Saiga, H., Nishida, H.
  • Journal Title: Developmental Cell Volume: 19 Pages: 589-598
  • Peer Reviewed
• [Journal Article] Limited functions of Hox genes in the larval development of the ascidian, Ciona intestinalis. (2010)
  • Author(s): Ikuta, T., Satoh, N., Saiga, H.
  • Journal Title: Developmental Volume: 137 Pages: 1505-1513
  • Peer Reviewed
• [Presentation] Cluster structure and developmental expression of Hox genes in the Halocynthia roretzi, a phylogentically remote ascidian from Ciona intestinalis. (2012)
  • Author(s): Sekigami, Y., Kobayashi,T., Omi, A., Ikuta, T., Fujiyama, A., Satoh, N.and Saiga, H
  • Organizer: Joint Meeting if the 45thAnnual Meeting of the Japanese Society of Developmental Biologists & the 64thAnnual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: Kobe
• [Presentation] ホヤの脳胞の発生におけるOtx遺伝子の転写制御機構と保存性の解析 Analysis of the transcriptional regulatory mechanism of Otx in the sensory vesicle development andof its conservation between two ascidian species (2012)
  • Author(s): 大沼耕平,西駕秀俊
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
• [Presentation] マボヤのHox遺伝子のクラスター構造と発現の解析 Analysis of the cluster structure and expression of Hox genes in the ascidian, Halocynthia roretzi. (2012)
  • Author(s): 関上由佳,小林拓矢,尾見歩惟,生田哲朗,藤山秋佐夫,佐藤矩行,西駕秀俊
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
• [Presentation] カタユウレイボヤにおける左右非対称な消化管形成過程の解析 Morphological analysis of the left-right asymmetric digestive tract formation in the ascidian, Ciona intestinalis (2012)
  • Author(s): 中澤啓一,山澤拓実,吉田慶太,小椋陽介,河合成道,笹倉靖徳,西駕秀俊
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
• [Presentation] Cluster structure and developmental expression of Hox genes in the Halocynthia roretzi, a phylogentically remote ascidian from Ciona intestinalis. (2012)
  • Author(s): Sekigami, Y., Kobayashi，T., Omi, A., Ikuta, T., Fujiyama, A., Satoh, N.and Saiga, H.
  • Organizer: Joint Meeting if the 45th Annual Meeting of the Japanese Society of Developmental Biologists & the 64th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: 神戸
• [Presentation] ホヤの脳胞の発生におけるOtx遺伝子の転写制御機構と保存性の解析 Analysis of the transcriptional regulatory mechanism of Otx in the sensory vesicle development and of its conservation between two ascidian species. (2012)
  • Author(s): 大沼耕平，西駕秀俊
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
• [Presentation] カタユウレイボヤにおける左右非対称な消化管形成過程の解析 Morphological analysis of the left-right asymmetric digestive tract formation in the ascidian, Ciona intestinalis. (2012)
  • Author(s): 中澤啓一，山澤拓実，吉田慶太，小椋陽介，河合成道，笹倉靖徳，西駕秀俊
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
• [Presentation] Repression of Rx gene on the left side of the sensory vesicle by Nodal signaling is crucial for right-sided formation of the ocellus photoreceptor in the development of Ciona intestinalis. (2011)
  • Author(s): Yoshida, K. and Saiga, H.
  • Organizer: The 6th International Tunicate Meeting
  • Place of Presentation: Montreal, Canada.
• [Presentation] マボヤのOtx 遺伝子の脳胞における転写調節機構の解析Analysis of transcription regulatory mechanisms of Otx in the developing central nervous system of the ascidian, Halocynthia roretzi (2011)
  • Author(s): 大沼耕平,西駕秀俊
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
• [Presentation] マボヤのHox遺伝子の解析 Analysis of Hox genes in the ascidian, Halocynthia roretzi (2011)
  • Author(s): 関上由佳,小林拓矢,尾見歩惟,生田哲朗,藤山秋佐夫,佐藤矩行,西駕秀俊
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
• [Presentation] カタユウレイボヤの消化管形成過程の解析Morphological analysis of the digestive tract formation in the ascidian, Ciona intestinalis. (2011)
  • Author(s): 山澤拓実,西駕秀俊
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
• [Presentation] マボヤのPitx の付着突起形成領域での発現はOtx により制御される Expression of Pitxin the papilla region is regulated by Otxin the development of the ascidian, Halocynthia roretzi (2011)
  • Author(s): 吉田慶太,上野元子,西駕秀俊
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
• [Presentation] Repression of Rx gene on the left side of the sensory vesicle by Nodal signaling is crucial for right-sided formation of the ocellus photoreceptor in the development of Ciona intestinalis (2011)
  • Author(s): Yoshida, K., Saiga, H.
  • Organizer: The 6th International Tunicate Meeting
  • Place of Presentation: モントリオール(カナダ)
• [Presentation] マボヤのOtx遺伝子の脳胞における転写調節機構の解析Analysis of transcription regulatory mechanisms of Otx in the developing central nervous system of the ascidian, Halocynthia roretzi (2011)
  • Author(s): 大沼耕平, 西駕秀俊
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
• [Presentation] マボヤのHox遺伝子の解析Analysis of Hox genes in the ascidian, Halocynthia roretzi (2011)
  • Author(s): 関上由佳, 小林拓矢, 尾見歩惟, 生田哲朗, 藤山秋佐夫, 佐藤矩行, 西駕秀俊
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
• [Presentation] カタユウレイボヤの消化管形成過程の解析Morphological analysis of the digestive tract formation in the ascidian, Ciona intestinalis (2011)
  • Author(s): 山澤拓実, 西駕秀俊
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
• [Presentation] マボヤのPitxの付着突起形成領域での発現はOtxにより制御されるExpression of Pitx in the papilla region is regulated by Otx in the development of the ascidian, Halocynthia roretzi (2011)
  • Author(s): 吉田慶太, 上野元子, 西駕秀俊
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
• [Presentation] Genomic analysis of the Hox cluster of an echinoderm, Oxycomanthas japonicas. (2010)
  • Author(s): Kondo, M., Tsurugaya, T., Sumiyoshi, N., Ikuta, T., Ota, T., Ikeo, K, Saiga, H., Akasaka, K.
  • Organizer: Genomic analysis of the Hox cluster of an echinoderm, Oxycomanthus japonicus.
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Limited functions of Hox genes in the larval development of the ascidian Ciona intestinalis. 4 (2010)
  • Author(s): Ikuta, T., Satoh, N. and Saiga, H.
  • Organizer: 43rdAnnual Meeting for the Japanese Society for Developmental Biologists jointly sponsored by the Asia-Pacific Developmental Biology Network
  • Place of Presentation: Kyoto
• [Presentation] Nodal signaling controls left-right asymmetric ocellus formation in Ciona intestinalis (2010)
  • Author(s): Yoshida, K. and Saiga, H
  • Organizer: 43rdAnnual Meeting for the Japanese Society for Developmental Biologists jointly sponsored by the Asia-Pacific Developmental Biology Network
  • Place of Presentation: Kyoto
• [Presentation] Limited functions of Hox genes in the larval development of the ascidian Ciona intestinalis. (2010)
  • Author(s): Ikuta, T., Satoh, N., Saiga, H.
  • Organizer: 43^<rd> Annual Meeting for the Japanese Society for Developmental Biologists jointly sponsored by the Asia-Pacific Developmental Biology Network
  • Place of Presentation: 京都
• [Presentation] Nodal signaling controls left-right asymmetric ocellus formation in Ciona intestinalis. (2010)
  • Author(s): Yoshida, K., Saiga, H.
  • Organizer: 43^<rd> Annual Meeting for the Japanese Society for Developmental Biologists jointly sponsored by the Asia-Pacific Developmental Biology Network
  • Place of Presentation: 京都
• [Presentation] 脊索動物ホヤにおいてHox10は消化管形成に必要な内胚葉索の細胞移動を制御する (2010)
  • Author(s): 河合成道, 小椋陽介, 吉田麗子, 濱田麻友子, 生田哲朗, 佐藤矩行, 西駕秀俊, 笹倉靖徳
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Remarks]
  • URL: http://www.biol.se.tmu.ac.jp/labo.asp?ID=devpro
• [Remarks]
  • URL: http://www.biol.se.tmu.ac.jp/labo.asp?ID=devpro