| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
grimp gene plays an important role in early stage of regeneration in Enchytraeus japonensis. grimp mRNA is detected transiently from 3 to 12 h post amputation only in mesodermal stem cells called neoblasts and mesodermal lineage cells. In order to clarify the function and cellular localization of grimp protein, we took approaches for the production, purification and characterization of recombinant protein. We also studied the physical role of grimp by introducing grimp-GFP fusion gene into mammalian stem cells. The results suggested that the half-life of the grimp-GFP was relatively short, there could be a mechanism for selective degradation of grimp protein.
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