| Project/Area Number |
22570210
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
NAKAYA Yukiko 独立行政法人理化学研究所, 初期発生研究チーム, 研究員 (70415256)
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| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Tetsuya 東京大学, 生産技術研究所, 講師 (90513359)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ライブイメージング / ニワトリ胚 / 中胚葉 / 細胞遊走 / 細胞極性 / ニワトリ初期胚 |
|---|
| Research Abstract |
During gastrulation, mesodermal cells migrate individually to become five lineage: axial, paraxial, intermediate, lateral and extraembryonic mesoderm. However, the precise cellular mechanism by which mesodermal cells migrate directionally toward their destination remained elusive. By in vivo imaging and tracking analysis with the gastrulating chicken embryo, we investigated the mode of cell movement and the positional relationship between nucleus and Golgi apparatus during migration. From this observation, we found that mesoderm cells move randomly and freely and show “nucleus front-Golgi back” polarity after they leave from the primitive streak. The perturbation of Golgi organization during migration resulted in the impaired movement of cells, suggesting that this front-back polarity is essential for mesoderm migration.
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