| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
We established B lymphoblastoid cell lines (EBV-B cell) from peripheral blood mononuclear cell stocks obtained from 550 South American indigenous individuals. We performed analysis of genetical relationships among tribes to make clear the migration rout of mongoloid populations from Eurasia to South America. Before starting genetical analysis, we checked the genomic stability of EBV-B cells during establishment and long-term culture periods, and showed that the cells retain stable genome structures by genetical studies. For the genetical analysis, samples were divided into 5 groups according to the geographical location, and then figured out the genetical features with forensic microsatellite markers. The results showed that each group retained smaller heterogeneity and specific allele frequency patterns for each microsatellite locus. These results suggest that Amerind populations are not so homogenous as a whole but can be divided into some genetically-diverse groups.
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