Study on the genetic factors involved in the tissue-culture responseof sugar beet (Beta vulgaris L.)

• Project/Area Number: 22580001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Hokkaido University
• Principal Investigator: KUBO Tomohiko 北海道大学, 大学院・農学研究院, 准教授 (40261333)
• Co-Investigator(Kenkyū-buntansha): TAGUCHI Kazunori 農業・食品産業技術総合研究機構, 北海道, 主任研究員 (80414754) ; MATSUHIRA Hiroaki 農業・食品産業技術総合研究機構, 北海道, 農業研究センター・研究員 (90549247)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 組織培養 / カルス化 / 再分化 / テンサイ / 遺伝子発現 / ダイアレル解析

Research Abstract

Sugar beet is known to be recalcitrant against callus induction andplant regeneration. Sugar-beet line NK-219mm-O is an exceptional line because it exhibitsexcellent response to tissue culture. Genetic basis of this tissue culture response wasinvestigated. NK-219mm-O was crossed with various sugar beet lines of poor tissue-cultureresponse. The resultant F1 plants exhibited good tissue-culture response. Nucleotidesequence of a gene whose expression is increased upon plant regeneration was obtained inthis study.

Research Products

• [Journal Article] Identification of the predominant nonrestoring allele for Owen-type cytoplasmic male sterility in sugar beet (Beta vulgaris L.): development of molecular markers for the maintainer genotype (2013)
  • Author(s): Moritani M, Taguchi K, Kitazaki K,Matsuhira H, Katsuyama T, Mikami T, KuboT
  • Journal Title: Molecular Breeding Volume: 32 Issue: 1 Pages: 91-100
  • DOI: 10.1007/s11032-013-9854-8
  • NAID: 120005451562
  • Peer Reviewed
• [Journal Article] Evolutionary aspects ofa unique internal mitochondrial targetingsignal in nuclear-migrated rps19 of sugarbeet (Beta vulgaris) (2013)
  • Author(s): Matsunaga M, Takahashi Y, Yui-Kurino R,Mikami T, Kubo T
  • Journal Title: Gene Volume: Vol.517 Pages: 19-26
  • Peer Reviewed
• [Journal Article] Evolutionary aspects of a unique internal mitochondrial targeting signal in nuclear-migrated rps19 of sugar beet (Beta vulgaris) (2013)
  • Author(s): Muneyuki Matsunaga
  • Journal Title: Gene Volume: 517 Issue: 1 Pages: 19-26
  • DOI: 10.1016/j.gene.2012.12.099
  • Peer Reviewed
• [Journal Article] Unusual and typicalfeatures of a novel restorer-of-fertilitygene of sugar beet (Beta vulgaris L.) (2012)
  • Author(s): Matsuhira H, Kagami H, Kurata M,Kitazaki K, Matsunaga M, Hamaguchi Y,Hagihara E, Ueda M, Harada M, Muramatsu A,Yui-Kurino R, Taguchi K, Tamagake H,Mikami T, Kubo T
  • Journal Title: Genetics Volume: Vol. 192 Pages: 1347-1358
  • Peer Reviewed
• [Journal Article] Is RNA editing implicated ingroup II intron survival in the angiosperm mitochondrial genome? (2012)
  • Author(s): Kagami H, Nagano H, Takahashi Y, MikamiT, Kubo T
  • Journal Title: Genome Volume: Vol. 55 Pages: 75-79
  • Peer Reviewed
• [Journal Article] Mitochondrialminisatellite polymorphisms in fodder andsugar beets reveal genetic bottlenecksassociated with domestication (2012)
  • Author(s): Yoshida Y, Matsunaga M, Cheng D, Xu D,Honma Y, Mikami T, Kubo T
  • Journal Title: BiologiaPlantarum Volume: Vol. 56 Pages: 369-372
  • Peer Reviewed
• [Journal Article] Unusual and typical features of a novel restorer-of-fertility gene of sugar beet (Beta vulgaris L.) (2012)
  • Author(s): Hiroaki Matsuhira
  • Journal Title: Genetics Volume: 192 Issue: 4 Pages: 1347-1358
  • DOI: 10.1534/genetics.112.145409
  • Peer Reviewed
• [Journal Article] Is RNA editing implicated in group II-intron survival in the angiosperm mitochondrial genome? (2012)
  • Author(s): H.Kagami
  • Journal Title: Genome Volume: 55 Issue: 1 Pages: 75-79
  • DOI: 10.1139/g11-080
  • Peer Reviewed
• [Journal Article] Identification and precise mapping ofresistant QTLs of Cercospora leaf spotresistance in sugar beet (Beta vulgaris L.), G 3 (2011)
  • Author(s): Taguchi K, Kubo T, Takahashi H, Abe H
  • Journal Title: Genes| Genomes| Genetics Volume: Vol. 1 Pages: 283-291
  • Peer Reviewed
• [Journal Article] A horizontally transferredtRNACys gene in the sugar beet mitochondrialgenome: evidence that the gene is presentin diverse angiosperms and its transcript is aminoacylated (2011)
  • Author(s): Kitazaki K, Kubo T, Kagami H, MatsumotoT, Fujita A, Matsuhira H, Matsuna ga M,Mikami T
  • Journal Title: The Plant Journal Volume: Vol. 68 Pages: 262-272
  • Peer Reviewed
• [Journal Article] Polymorphicminisatellites in the mitochondrial DNAsof Oryza and Brassica (2011)
  • Author(s): Honma Y, Yoshida Y, Terachi T, ToriyamaK, Mikami T, Kubo T
  • Journal Title: Current Genetics Volume: Vol.57 Pages: 261-270
  • Peer Reviewed
• [Journal Article] Kubo T,Large 3' UTR of sugar beet rps3 istruncated in cytoplasmic male-sterile mitochondria (2011)
  • Author(s): Matsunaga M, Nagano H, Mikami T
  • Journal Title: Plant Cell Reports Volume: Vol. 30 Pages: 231-238
  • Peer Reviewed
• [Journal Article] A horizontally transferred tRNA^<cys> gene in the sugar beet mitochondrial genome : evidence that the gene is present in diverse angiosperms and its transcript is aminoacylated (2011)
  • Author(s): K.Kitazaki
  • Journal Title: The Plant Journal Volume: 68 Issue: 2 Pages: 262-272
  • DOI: 10.1111/j.1365-313x.2011.04684.x
  • Peer Reviewed
• [Journal Article] Large 3' UTR of sugar beet rps3 is truncated in cytoplasmic male-sterile mitochondria (2011)
  • Author(s): Muneyuki Matsunaga
  • Journal Title: Plant Cell Reports Volume: 30 Pages: 231-238
  • Peer Reviewed
• [Journal Article] Mitochondrial genome diversity in Beta vulgaris L.ssp vulgaris (Leaf and Garden Beet Groups) and its implications concerning the dissemination of the rop (2011)
  • Author(s): Dayou Cheng
  • Journal Title: Genetic Resources and Crop Evolution Volume: 58 Pages: 553-560
  • Peer Reviewed
• [Journal Article] Identification and precise mapping of resistant QTLs of Cercospora leaf spot resistance in sugar beet (Beta vulgaris L.) (2010)
  • Author(s): Kazunori Taguchi
  • Journal Title: G3 : Genes Genomes Genetics Volume: 1 Issue: 4 Pages: 283-291
  • DOI: 10.1534/g3.111.000513
  • Peer Reviewed
• [Journal Article] Increased accumulation of intron-containing transcripts in rice mitochondria caused by low temperature : is cold-sensitive RNA editing implicated? (2010)
  • Author(s): Shiho Kurihara-Yonemoto
  • Journal Title: Current Genetics Volume: 56 Pages: 529-541
  • Peer Reviewed
• [Presentation] Production of transformation-competent sugar beet (Beta vulgaris L.) with annual habitat: a basis for sugar beet research (2012)
  • Author(s): Kagami H, Matsuhira H, Taguchi K, Kubo T, Mikami T
  • Organizer: International Conference on Agricultural Biodiversity and Sustainability 2012
  • Place of Presentation: Hokkaido University
  • Year and Date: 2012-08-29
• [Presentation] テンサイOwen型細胞質雄性不稔性における花粉稔性回復遺伝子Rf1のプロモーター解析 (2012)
  • Author(s): 鏡豊代、田口和憲、久保友彦、三上哲夫
  • Organizer: 日本育種学会第121回講演会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2012-03-30
• [Presentation] 高度培養適性を保持するテンサイ系統において再分化過程で特異的に発現する遺伝子の探索 (2011)
  • Author(s): 松平洋明、鏡豊代、田口和憲、久保友彦、三上哲夫
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井県立大学
  • Year and Date: 2011-09-23
• [Presentation] 形質転換可能な一年生テンサイ作出の試み:テンサイ生殖研究の基盤研究 (2011)
  • Author(s): 鏡豊代、松平洋明、久保友彦、三上哲夫
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井県立大学
  • Year and Date: 2011-09-23
• [Presentation] テンサイカルスからの再分化能は遺伝的制御下にある (2010)
  • Author(s): 鏡豊代、松平洋明、久保友彦、三上哲夫
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] テンサイカルスからの再分化能は遺伝的制御下にある (2010)
  • Author(s): 鏡豊代
  • Organizer: 日本分子生物学会第33回年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] Production of transformation-competent sugar beet (Beta vulgaris L.) with annual habitat: a basis for sugar beet research
  • Author(s): Hiroyo Kagami
  • Organizer: International Conference on Agricultural Biodiversity and Sustainability 2012
  • Place of Presentation: Hokkaido University, Sapporo