Metabolic engineering of fatty acidcomposition in soybean by using TILLING
Project/Area Number |
22580006
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Breeding science
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Research Institution | Saga University |
Principal Investigator |
ANAI Toyoaki 佐賀大学, 農学部, 准教授 (70261774)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
Keywords | 突然変異 / 逆遺伝学 / ダイズ / TILLING / 脂肪酸 / 突然変異体 / TILLING法 |
Research Abstract |
TILLING is one of the most attractive methods for screening novel mutant lines with reverse genetics approach in recent years.Inthis study aimed at improving fatty acid composition of soybean oil by metabolic engineering, I have tried to isolate novel mutant lines and to combine their mutant alleles. As a result, I have isolated several mutant lines corresponding to acyl-ACP thioesterase, stearoyl-ACP desaturase and microsomal omega-3 fatty acid desaturase genes, and successfully developed novel fatty acid mutant lines with more than 80% oleic acid or less than 1.5% linolenic acid in seed oil.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] 2013 年 Genetic Variation in Soybean at the Maturity Locus E4 Is Involved in Adaptation to Long Days at High Latitudes Agronomy2013
Author(s)
Y. Tsubokura, H. Matsumura, M. Xu, B. Liu, H. Nakashima, T. Anai, F. Kong, X. Yuan, H. Kanamori, Y. Katayose, R. Takahashi, K. Harada and J. Abe
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Journal Title
Agronomy
Volume: 3
Issue: 1
Pages: 117-134
DOI
Related Report
Peer Reviewed
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[Journal Article] 2012 年 Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1, which regulates photoperiodic flowering2012
Author(s)
Z. Xia, S. Watanabe, T. Yamada, Y. Tsubokura, H. Nakashima, H. Zhai, T. Anai, S. Sato, T. Yamazaki, S. Lu, H. Wu, S. Tabata and K. Harada
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Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 109
Issue: 32
Pages: 2155-2164
DOI
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Peer Reviewed
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[Journal Article] Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1, which regulates photoperiodic flowering2012
Author(s)
Z.Xia, S.Watanabe, T.Yamada, Y.Tsubokura, H.Nakashima, H.Zhai, T.Anai. S.Sato, T.Yamazaki, S.Lu, H.Wu, S.Tabata, K.Harada
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Journal Title
Natl.Acad.Sci.USA
Volume: (in press)
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Peer Reviewed
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[Journal Article] 2011 年 A map-based cloning strategy employing a residual heterozygous line reveals that the GIGANTEA gene is involved in soybean maturity and flowering2011
Author(s)
S. Watanabe, Z. Xia, R. Hideshima, Y. Tsubokura, S. Sato, N. Yamanaka, R. Takahashi, T. Anai, S. Tabata, K. Kitamura, K. Harada
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Journal Title
Genetics
Volume: 188
Issue: 4
Pages: 395-407
DOI
Related Report
Peer Reviewed
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[Journal Article] A mapbased cloning strategy employing a residual heterozygous line reveals that the GIGANTEA gene is involved in soybean maturity and flowering2011
Author(s)
S.Watanabe, Z.Xia, R.Hideshima, Y.Tsubokura, S.Sato, N.Yamanaka, R.Takahashi, T.Anai, S.Tabata, K.Kitamura, K.Harada
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Journal Title
Genetics
Volume: 188
Issue: 2
Pages: 395-407
DOI
Related Report
Peer Reviewed
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[Journal Article] A map-based cloning strategy employing a residual heterozygous line reveals that the GIGANTEA gene is involved in soybean maturity and flowering.2011
Author(s)
S.Watanabe, Z.Xia, R.Hideshima, Y.Tsubokura, S.Sato, N.Yamanaka, R.Takahashi, T.Anai, S.Tabata, K.Kitamura, K.Harada
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Journal Title
Related Report
Peer Reviewed
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[Book] Soybean2011
Author(s)
K.Harada, S.Watanabe, Z.Xia, Y.Tsubokura, N.Yamanaka, T.Anai
Publisher
InTech Publishers
Related Report
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