Molecular mechanisms for cadmium translocation in rice

• Project/Area Number: 22580008
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Akita Prefectural University
• Principal Investigator: AKAGI Hiromori 秋田県立大学, 生物資源科学部, 教授 (50315587)
• Co-Investigator(Kenkyū-buntansha): WATANABE Akio 秋田県立大学, 生物資源科学部, 准教授 (50325940) ; NAKAZAWA Nobushige 秋田県立大学, 生物資源科学部, 教授 (40315586)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 植物分子育種 / イネ / カドミウム / ファイトレメディエーション / 膜輸送体 / OsHMA3

Research Abstract

In rice, cadmium (Cd) taken up in the cytoplasm of root cells is sequestrated into the vacuole of the cells via OsHMA3, then loading to the xylem and translocation to shoots of Cd are limited. By contrast, in Cd over-accumulating rice, Cd remains at a higher concentration in the cytoplasm due to the lack of OsHMA3 function, and is available for loading to the xylem. This vacuole sequestrating by OsHMA3 is a key for over-accumulation of Cd in rice shoots. And we also demonstrated that the overexpression of OsHMA3 allows control distribution of Cd in plants.

Research Products

• [Journal Article] Mutations in rice (Oryza sativa) heavy metal ATPase 2 (OsHMA2) restrict the translocation of Zn and Cd (2012)
  • Author(s): Satoh-Nagasawa N, Mori M, Nakazawa N, Kawamoto T, Nagato Y, Sakurai K, Takahashi H, Watanabe A, Akagi H
  • Journal Title: Plant and Cell Physiology Volume: 53 Pages: 213-224
  • Peer Reviewed
• [Journal Article] 、OsHMA3, a P1B -type of ATPase affects root-to-shoot cadmium translocation in rice by mediating efflux into vacuoles (2011)
  • Author(s): Miyadate H., Adachi S., Hiraizumi A., Tezuka K., Nakazawa N., Kawamoto T., Katou K., Kodama I., Sakurai K., Takahashi H., Satoh N., Watanabe A., Fujimura T., Akagi H.
  • Journal Title: New Phytologis Volume: 189 Pages: 190-199
  • Peer Reviewed
• [Journal Article] Isolation of novel types of Arabidopsis mutants with altered re actions to cadmium: cadmium-gradient agar plates are an effective screen for the heavy metal-related mutants (2011)
  • Author(s): Watanabe A, Ito H, Chiba M, Ito A, Shimizu H, Fuji S, Nakamura S, Hattori H, Chino M, Satoh N, Takahashi H, Sakurai K, Akagi H
  • Journal Title: Planta Volume: 232 Pages: 825-836
  • Peer Reviewed
• [Journal Article] OsHMA3, a P_<1B>-type of ATPase affects root-to-shoot cadmium translocation in rice by mediating efflux into vacuoles (2011)
  • Author(s): Miyadate H, Adachi S, Hiraizumi A, Tezuka K, Nakazawa N, Kawamoto T, Katou K, Kodama I, Sakurai K, Takahashi H, Satoh-Nagasawa N, Watanabe A, Fujimura T, Akagi H
  • Journal Title: New Phytologisit Volume: 189 Pages: 190-199
  • Peer Reviewed
• [Journal Article] A single recessive gene controls cadmium translocation in the cadmium hyperaccumulating rice cultivar Cho-Ko-Koku (2010)
  • Author(s): Tezuka K., Miyadate H., Kato K., Kodama I., Matsumoto S., Kawamoto T., Masaki S., Satoh H., Yamaguchi M., Sakurai K., Takahashi H., Satoh N., Watanabe A., Fujimura T., Akagi H.
  • Journal Title: Theoretical and Applied Genetics Volume: 120 Pages: 1175-1182
  • Peer Reviewed
• [Presentation] イネ属の A ゲノム野生種における OsHMA3 遺伝子の進化とカドミウム移行の多様性 (2013)
  • Author(s): 熊谷さおり、堀川悠佑、相馬美沙子、佐藤奈美子、高橋秀和、櫻井健二、渡辺明夫、赤木宏守
  • Organizer: 日本育種学会第123回講演会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2013-03-28
• [Presentation] イネ属のAゲノム野生種におけるOsHMA3遺伝子の進化とカドミウム移行の多様性 (2013)
  • Author(s): 熊谷さおり・堀川悠佑・相馬美沙子・佐藤奈美子・高橋秀和・櫻井健二・渡辺明夫・赤木宏守
  • Organizer: 日本育種学会 第123回講演会
  • Place of Presentation: 東京農業大学（東京都）
• [Presentation] イネのカドミウム輸送体(OsHMA3)の C 末端領域の機能解析 (2012)
  • Author(s): 熊谷さおり、鈴木辰弥、佐藤奈美子、高橋秀和、櫻井健二、渡辺明夫、赤木宏守
  • Organizer: 日本育種学会第122回講演会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-09-01
• [Presentation] イネの OsHMA3 の構造がカドミウム輸送に及ぼす影響 (2012)
  • Author(s): 熊谷さおり、鈴木辰弥、佐藤奈美子、高橋秀和、櫻井健二、渡辺明夫、赤木宏守
  • Organizer: 第7回東北育種研究集会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2012-08-20
• [Presentation] イネのカドミウム輸送体（OsHMA3）のC末端領域の機能解析 (2012)
  • Author(s): 熊谷さおり・鈴木辰弥・佐藤奈美子・高橋秀和・櫻井健二・渡辺明夫・赤木宏守
  • Organizer: 日本育種学会 第122回講演会
  • Place of Presentation: 京都産業大学（京都市）
• [Presentation] イネのOsHMA3の構造がカドミウム輸送に及ぼす影響 (2012)
  • Author(s): 熊谷さおり・鈴木辰弥・佐藤奈美子・高橋秀和・櫻井健二・渡辺明夫・赤木宏守
  • Organizer: 第7回 東北育種研究集会
  • Place of Presentation: 秋田県立大学（秋田市）
• [Presentation] イネの液胞への重金属輸送体(OsHMA3)はイネのカドミウム移行を制御する (2010)
  • Author(s): 宮舘秀典、手塚耕一、佐藤奈美子、櫻井健二、高橋秀和、渡辺明夫、赤木宏守
  • Organizer: 第5回東北育種研究集会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-08-26
• [Presentation] イネの液胞への重金属輸送体(OsHMA3)はカドミウムの地上部への移行を制御する (2010)
  • Author(s): 宮舘秀典、手塚耕一、佐藤奈美子、櫻井健二、高橋秀和、渡辺明夫、赤木宏守
  • Organizer: 第5回 東北育種研究集会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-08-26