Transcriptome analysis of wheat and Fusarium graminearum for Fusarium head blight resistance in wheat.

• Project/Area Number: 22580009
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Yokohama City University
• Principal Investigator: BAN Tomohiro 横浜市立大学, 付置研究所, 教授 (80343771)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 赤かび病抵抗性 / 遺伝子発現 / マイコトキシン / QTL / マイクロアレイ / 植物育種・遺伝 / 抵抗性・耐性 / コムギ / フザリウム菌 / クロストーク

Research Abstract

Fusarium graminearum causes a critical wheat disease Fsarium head blight (FHB) produces mycotoxyn such as DON and NIV with highly expression of Tri genes during their initial infection to wheat spikes. We analyzed the three Tri genes expression (Tri5, Tri6 and Tri10) by RT-PCR in the infected spikes of five wheat varieties with different level of HB resistance. The Tri genes expression was influenced by the wheat genotype which was highly induced resistance varieties in the early stage of infection and no induction in the susceptible varieties along with their FHB resistance level. It revealed that FHB resistance wheat genotype has active defense mechanism to cross-talk with Fusarium gene expressions.

Research Products

• [Journal Article] Genomics and breeding of Brassicaceae crops (2014)
  • Author(s): Hoai T. T. T., Matsusaka, H., Toyosawa, Y., Suu T. D., Satoh H., Kumamaru T.
  • Journal Title: Breeding Science Volume: 64 Issue: 1 Pages: 1-1
  • DOI: 10.1270/jsbbs.64.1
  • NAID: 130004146416
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed
• [Journal Article] Characterization of a wheat transcription factor, TaWRKY45, and its effect on Fusarium head blight resistance in transgenic wheat plants (2011)
  • Author(s): Insaf Bahrini, Motoki Sugisawa, Rie Kikuchi, Taiichi Ogawa, Hiroyuki Kawahigashi, Tomohiro Ban and Hirokazu Handa
  • Journal Title: Breeding Science Volume: 61(2) Pages: 121-129
  • NAID: 10029135165
  • Peer Reviewed
• [Journal Article] EvoTree; Software for easily drawing the pedigree chart with varietal characteristics (2011)
  • Author(s): Akane Takesaki, Tomohiro Ban, Masumi Katsuta
  • Journal Title: Breeding Research Volume: 13(in Japanese) Pages: 19-24
  • NAID: 10028099215
  • Peer Reviewed
• [Journal Article] Genetic relationships between resistance to Fusarium head blight and crown rot in bread wheat (Triticum aestivum L.) (2010)
  • Author(s): Hao Bing Li, Guo Qiang Xie, Jun Ma, Gui Ru Liu, Shu Min Wen, Tomohiro Ban, Sukumar Chakraborty, and Chun Ji Liu
  • Journal Title: Theoretical and Applied Genetics Volume: 121 Pages: 941-950
  • Peer Reviewed
• [Journal Article] Deoxynivalenol Distribution in Flour and Bran of Spring Wheat Lines with Different Levels of Fusarium Head Blight Resistance (2010)
  • Author(s): Zenta Nishio, Kanenori Takata, Miwako Ito, Masahiko Tanio, Tadashi Tabiki, Hiroaki Yamauchi, and Tomohiro Ban
  • Journal Title: Plant Disease March 2010 Volume: Volume 94, Number 3 Issue: 3 Pages: 335-338
  • DOI: 10.1094/pdis-94-3-0335
  • Peer Reviewed
• [Presentation] Genomic characterization of Afghanistan wheat landraces (2014)
  • Author(s): Tomohiro Ban and Alagu Manickavelu
  • Organizer: Plant Genome Asia
  • URL: http://www.globalengage.co.uk/pgcasia/PGCA_Agenda.pdf
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Invited
• [Presentation] Genomic characterization of Afghan wheat landraces (2014)
  • Author(s): Tomohiro Ban and Alagu Manickavelu
  • Organizer: Plant Genomics Asia
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Invited
• [Presentation] Moleculara pathogenicity of wheat -Fusarium graminearum interaction (2013)
  • Author(s): Ayumi Kosaka, Alagu Manikavelu, Daniela Kajihara, Tomohiro Ban
  • Organizer: 12^<th> International Wheat genetic Symposium
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] コムギ生体内における赤かび病菌F. graminearum毒素合成遺伝子の発現プロファイリング (2013)
  • Author(s): 原田萌・坂智広
  • Organizer: 日本育種学会第124回講演会
  • Place of Presentation: 鹿児島大学
• [Presentation] Molecular pathogenicity of wheat-Fusarium graminearum interaction (2013)
  • Author(s): 原田Ayumi Kosaka, Alagu Manickavelu, Daniela Kajihara, Tomohiro Ban 萌，坂 智広
  • Organizer: 12th International Wheat Genetic Symposium
  • Place of Presentation: パシフィコ横浜、横浜市
• [Presentation] コムギ生体内における赤かび病菌 F. graminearum 毒素合成遺伝子発現のプロファイリング (2013)
  • Author(s): 原田 萌，坂 智広
  • Organizer: USWBSI the 2013 Nati日本育種学会 第124回講演会onal Fusarium Head Blight Forum
  • Place of Presentation: 鹿児島大学
• [Presentation] MOLECULAR PATHOGENICITY OF WHEAT & FUSARIUM GRAMINEARUM INTERACTION (2013)
  • Author(s): Ayumi Kosaka, Alagu Manickavelu, Daniela Kajihara and Tomohiro Ban
  • Organizer: USWBSI the 2013 National Fusarium Head Blight Forum
  • Place of Presentation: Milwaukee, Wisconsin, USA
• [Presentation] Contribution of TaMRP-D1 allele on Qfhs.kibr-2DS in wheat for FHB resistance (2012)
  • Author(s): Niwa S, Kikuchi R, Kubo K, Lewis J, Nitta M, Nasuda S, Handa H, Ban T
  • Organizer: 4th International Symposium on Fusarium Head Blight
  • Place of Presentation: Nanjing
• [Presentation] 日本のコムギ遺伝資源コレクションにおける赤かび病抵抗性の評価 (2012)
  • Author(s): 丹羽紗也佳、菊地理絵、久保堅司、ルイス・ジャネット、新田みゆき、那須田周平、半田裕一、坂智広
  • Organizer: 日本育種学会第122回講演会
  • Place of Presentation: 京都
• [Presentation] コムギにおけるTaMRP-D1遺伝子発現が赤かび病毒素蓄積に及ぼす効果 (2012)
  • Author(s): 杉澤幹起、菊地理絵、小川泰一、半田裕一、坂智広
  • Organizer: 日本育種学会第122回講演会
  • Place of Presentation: 京都
• [Presentation] Discovery of superior Sumai 3 for increasing DON tolerance combined resistance allele for multidrug resistance-associated protein (MRP) as a candidate gene for'Qfhs.kibr-2DS'to reduce DON accumulation in wheat grains (2010)
  • Author(s): Niwa S, Kikuchi R, Handa H, Ban T
  • Organizer: European Fusarium Seminar'Fusarium–Mycotoxins, Taxonomy, Pathogenicety and Host Resistance
  • Place of Presentation: ワルシャワ(ポーランド)
  • Year and Date: 2010-09-23
• [Presentation] Isolation of wheat multidrug resistance-associated protein gene (TaMRP1), a possible candidate of the multiple traits QTL for the Fusarium head blight resistance (2010)
  • Author(s): Kikuchi R, Niwa S, Ogawa T, Handa H, Ban T
  • Organizer: European Fusarium Seminar'Fusarium–Mycotoxins, Taxonomy, Pathogenicety and Host Resistance'
  • Place of Presentation: ワルシャワ(ポーランド)
  • Year and Date: 2010-09-23
• [Presentation] Discovery of snperior Sumai 3 for increasing DON tolerance combined resistance allele for multidrug resistance-associated protein (MRP) as a candidate gene for 'Qfhs.kibr-2DS'to reduce DON accumulation in wheat grains (2010)
  • Author(s): Niwa S, Kikuchi R, Handa H, Ban T
  • Organizer: European Fusarium Seminaz 'Fusarium-Mycotoxins, Taxonomy, Pathogenicety and Host Resistance'
  • Place of Presentation: Plant Breeding and Acclimatization Institute (IHAR)、ワルシャワ(ポーランド)
  • Year and Date: 2010-09-23
• [Presentation] Isolation of wheat multidrug resistance-associated protein gene(TaMRP1), a possible candidate of the multiple traits QTL for the Fusarium head blight resistance. (2010)
  • Author(s): Kikuchi R, Niwa S, Ogawa T, Handa H, Ban T
  • Organizer: European Fusarium Seminaz 'Fusarium-Mycotoxins, Taxonomy, Pathogenicety and Host Resistance'
  • Place of Presentation: Plant Breeding and Acclimatization Institute (IHAR)、ワルシャワ(ポーランド)
  • Year and Date: 2010-09-23
• [Remarks]
  • URL: http://pgsource.sci.yokohama-cu.ac.jp/index.html