Isolation and functional analysis of acid soil tolerance genes in rice, which are useful for genome based breeding of environmentally sustainable varieties.

• Project/Area Number: 22580010
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: The University of Shiga Prefecture
• Principal Investigator: SHIMIZU Akifumi 滋賀県立大学, 環境科学部, 准教授 (40409082)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 抵抗性・耐性 / バイオレメディエーション / ストレス耐性 / 遺伝子単離

Research Abstract

Acid soils limit crop yields around the world due to nutrients deficiency and mineral toxicity. Improving acid soil tolerant rice is very promising for environmentally sustainable agriculture. Towards genome-based breeding for acid soil, chromosomal region search and/or gene isolation of three kinds of traits, which are related to proton toxicity and phosphorus deficiency, were conducted. Tolerant alleles of isolated genes were introgressed to major variety Koshihikari and their effects wereobserved.

Research Products

• [Journal Article] Discovery of high-confidence single nucleotide polymorphisms from large-scale de novo analysis of leaf transcripts of Aegilops tauschii, a wild wheat progenitor (2012)
  • Author(s): Iehisa JCM, Shimizu A, Sato K, Nasuda S, Takumi S
  • Journal Title: DNA Research Volume: 19 Issue: 6 Pages: 487-497
  • DOI: 10.1093/dnares/dss028
  • NAID: 120005398237
  • Peer Reviewed
• [Journal Article] Suppression ofa NAC-like transcription factor gene improves boron-toxicity tolerance in rice(Oryza sativa L.) (2011)
  • Author(s): Ochiai K, ShimizuA., OkumotoY, Fujiwara T, Matoh T.
  • Journal Title: Plant Physiology Volume: 156 Pages: 1457-1463
  • Peer Reviewed
• [Journal Article] Suppression of a NAC-Like Transcription Factor Gene Improves Boron- Toxicity Tolerance in Rice (2011)
  • Author(s): Ochiai K, Shimizu A, Okumoto Y, Fujiwara T, Mato, T.
  • Journal Title: Plant Physiol Volume: 156(3) Issue: 3 Pages: 1457-1463
  • DOI: 10.1104/pp.110.171470
  • Peer Reviewed
• [Presentation] リン欠乏ストレス下におけるイネ葉身中のリン再利用2 (2010)
  • Author(s): 片山敬仁、 小川恵未、 植川英明、 清水顕史、長谷川博、 落合久美子、 間藤徹、
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2010-09-24
• [Presentation] イネ根の Iron-plaque 非形成変異体を用いた、mPing 転移断片の塩基配列 (2010)
  • Author(s): 松本公佑、 西村悠希、 清水顕史、 長谷川博、奥本裕、
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2010-09-24
• [Presentation] イネ根のIron-plaque非形成変異体を用いた、mPing転移断片の塩基配列 (2010)
  • Author(s): 松本公佑, ら
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2010-09-24