Plant regeneration promoted by complete decapitation and controlled phenol metabolism in vitro
Project/Area Number |
22580038
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Horticulture/Landscape architecture
|
Research Institution | Osaka Prefecture University |
Principal Investigator |
ODA Masayuki 大阪府立大学, 大学院・生命環境科学研究科, 教授 (50291604)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 野菜 / 植物組織培養法 / 再分化 / シュート形成 / 遺伝子組換え / 褐変抑制 / 増殖 / 窒素 / サイトカイニン / 全茎切断 / 遺伝子組み換え / 褐色抑制 |
Research Abstract |
Tissue culture is effective in the study of plant science and breeding, but has not been established and unstable for regeneration. We developed a new tissue culture method in which hypocotyl is cut under cotyledons of a plant in vitro. When plant grown on a nitrogen deficient medium is cut by the method, and transplanted into a medium including a reagent to control browning (AOPP) promotes the regeneration with stability. These results show a possible adaptation of the method to many plant species.
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Report
(4 results)
Research Products
(16 results)