Plant regeneration promoted by complete decapitation and controlled phenol metabolism in vitro

• Project/Area Number: 22580038
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Osaka Prefecture University
• Principal Investigator: ODA Masayuki 大阪府立大学, 大学院・生命環境科学研究科, 教授 (50291604)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 野菜 / 植物組織培養法 / 再分化 / シュート形成 / 遺伝子組換え / 褐変抑制 / 増殖 / 窒素 / サイトカイニン / 全茎切断 / 遺伝子組み換え / 褐色抑制

Research Abstract

Tissue culture is effective in the study of plant science and breeding, but has not been established and unstable for regeneration. We developed a new tissue culture method in which hypocotyl is cut under cotyledons of a plant in vitro. When plant grown on a nitrogen deficient medium is cut by the method, and transplanted into a medium including a reagent to control browning (AOPP) promotes the regeneration with stability. These results show a possible adaptation of the method to many plant species.

Research Products

• [Journal Article] L-2-amynooxy-3-phenylpropionic acid (AOPP) promoting shoot formation from hypocotyl explant of eggplant (2013)
  • Author(s): Tanaka, H., H. Ueda, K. Mitsukuri, T. Tezuka, S. Shiozaki, M. Oda
  • Journal Title: Research Journal of Botany Volume: 8 Pages: 4-8
  • Peer Reviewed
• [Journal Article] L-2-Aminooxy-3-phenylpropionic acid (AOPP) promoting shoot formation from hypocotyl explants of eggplant (2012)
  • Author(s): Hideyuki Tanaka, Haruka Ueda, Kazuhiko Mitsukuri, Takahiro Tezuka, Shuji Shiozaki and Masayuki Oda
  • Journal Title: Res. J. Biotech Volume: 8 Pages: 4-8
  • Peer Reviewed
• [Journal Article] Effect of ascorbic acid and etiolation on antioxidant enzyme activity and phenylpropanoid metabolism during shoot regeneration from cut ends of tomato stems. J. Japan (2011)
  • Author(s): Masahum Johkan, Yoshihiro Imahori, Hajime Furukawa, Mitsukuri, Kazuhiko, Satoshi Yamasaki, Hidekazu Tanaka, Masayuki Oda
  • Journal Title: Soc. Hort. Sci. Pages: 45-51
  • Peer Reviewed
• [Journal Article] フウランの増殖における AOPP の効果 (2011)
  • Author(s): 箕作和彦・小田雅行
  • Journal Title: 植 Volume: 45 Pages: 180-185
• [Journal Article] フウランの増殖におけるAOPPの効果 (2011)
  • Author(s): 箕作和彦・小田雅行
  • Journal Title: 植調 Volume: 45 Pages: 180-185
• [Journal Article] Effect of ascorbic acid and etiolation on antioxidant enzyme activity and phenylpropanoid metabolism during shoot regeneration from cut ends of tomato stems (2011)
  • Author(s): Johkan, Masahumi, Yoshihiro Imahori, Hajime Furukawa, Mitsukuri, Kazuhiko, Satoshi Yamasaki, Hidekazu Tanaka, Masayuki Oda
  • Journal Title: J.Japan.Soc.Hort.Sci Volume: 80 Pages: 45-51
  • NAID: 130004951630
  • Peer Reviewed
• [Journal Article] L-2-aminooxy-3-phenylpropionic acid (AOPP) controls browning and promotes adventitious bud formation in Neofinetia falcata in vitro. J. Japan (2010)
  • Author(s): Kazuhiko Mitsukuri, Masahumi Johkan, Satoshi Yamasaki, Hidekazu Tanaka, Takahiro Tezuka, Kei-ichiro Mishiba, Masayuki Oda
  • Journal Title: Soc. Hort. Sci. Pages: 367-371
  • Peer Reviewed
• [Journal Article] L-2-aminooxy-3-phenylpropionic acid (AOPP) controls browning and promotes adventitious bud formation in Neofinetia falcata in vitro (2010)
  • Author(s): Mitsukuri, Kazuhiko, Masahumi Johkan, Satoshi Yamasaki, Hidekazu Tanaka, Takahiro Tezuka, Kei-ichiro Mishiba, Masayuki Oda
  • Journal Title: J.Japan.Soc.Hort.Sci Volume: 79 Pages: 367-371
  • NAID: 130004951619
  • Peer Reviewed
• [Presentation] ナスのin vitro 全茎切断における窒素欠乏前処理がシュート形成に及ぼす影響 (2013)
  • Author(s): 田中秀幸・藤岡ももこ・黒田恵子・古川一・手塚孝弘・小田雅行
  • Organizer: 平成25年度園芸学会春季大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2013-03-24
• [Presentation] ナスおよびピーマンのin vitro 全茎切断におけるAOPP 処理が不定芽形成に及ぼす影響 (2013)
  • Author(s): 廣瀬亮平・藤岡ももこ・田中秀幸・手塚孝弘・小田雅行
  • Organizer: 平成25年度園芸学会春季大会
  • Place of Presentation: 東京農工大学.
  • Year and Date: 2013-03-24
• [Presentation] ナスおよびピーマンのin vitro 全茎切断におけるAOPP 処理が不定芽形成に及ぼす影響 (2013)
  • Author(s): 廣瀬亮平・藤岡ももこ・田中秀幸・手塚孝弘・小田雅行
  • Organizer: 平成25年度園芸学会春季大会
  • Place of Presentation: 東京農工大学
• [Presentation] Adventitious bud formation promoted by L-2-aminooxy-3-phenylpropionic acid in tomato plants treated with the complete decapitation method in vitro (2012)
  • Author(s): Hideyuki Tanaka , Kazuhiko Mitsukuri,Satoshi Yamasaki, Ryohei Hirose, TakahiroTezuka, and Masayuki Oda
  • Organizer: ASHS annual conference
  • Place of Presentation: Inter Continental Miami, USA.
  • Year and Date: 2012-08-03
• [Presentation] A simple and efficient method for shoot regeneration from hypocotyls in eggplant in vitro (2011)
  • Author(s): Hideyuki Tanaka, Yuta Yokoyama, Satoshi Yamasaki, Takahiro Tezuka, Masayuki Oda
  • Organizer: 8thSolancea and 2ndCucurbitacea Genome Joint Conference
  • Place of Presentation: Kobe Convention Center, Japan
  • Year and Date: 2011-12-02
• [Presentation] ナスの胚軸培養における PAL 阻害剤 AOPP による不定芽形成の促進 (2011)
  • Author(s): 田中秀幸・上田晴香・箕作和彦・手塚孝弘・小田雅行
  • Organizer: 園芸学会平成 23 年度秋季大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-26
• [Presentation] トマトの in vitro 全茎切断における PAL 阻害剤 AOPP による不定芽形成の促進 (2011)
  • Author(s): 箕作和彦・田中秀幸・山崎識知・手塚孝弘・小田雅行
  • Organizer: 園芸学会平成 23 年度秋季大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-26
• [Presentation] A simple and efficient method for shoot regeneration from hypocotyls in eggplant in vitro (2011)
  • Author(s): H.Tanaka, Y.Yokoyama, S.Yamasaki, T.Tezuka, M.Oda
  • Organizer: 8^<th> Solancea and 2^<nd> Cucurbitacea Genome Joint Conference
  • Place of Presentation: Kobe Convention Center, Japan