Anaiysis of cell wall hydrolase genes involving to applemealy texture

• Project/Area Number: 22580040
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Nihon University
• Principal Investigator: TATEISHI Akira 日本大学, 生物資源科学部, 准教授 (30267041)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 多糖類 / 果実の軟化 / 細胞壁 / アラビノース / α-アラビノフラノシダーゼ / 酵素

Research Abstract

Total RNA was isolated from apple fruit, which showing mealytexture. Substantial anlout of polysaccharides was contained in the RNA sample. A putative a-L-arabinofuranosidase gene associated with mealy apple was reported.Substrate speeificities ofα-L-arabinofuranosidases expressed in plant cultured cells wereshown.

Research Products

• [Journal Article] Heterologous expres- sion of tomato glycoside hydrolase family 3 α-L-arabinofuranosidase/β-xylosidases in tobacco suspension cultured cells and synergic action of a family 51 isozyme under antisense suppression of the enzyme (2013)
  • Author(s): Akira Tateishi, Yusuke Kamiyoshihara, Junko Matsuno, Fumika Miyohashi, Hajime Shiba, Yoshinori Kanayama, Keiichi Watanabe, Kazunari Nomura, Hiroaki Inoue
  • Journal Title: Physiologia Plantarum Volume: (In press)
• [Presentation] 花の開花ステージにおけるα-アラビノフラノシダーゼ遺伝子の発現 (2012)
  • Author(s): 吉本圭吾、立石亮、渡辺慶一、井上弘明
  • Organizer: 園芸学会
  • Place of Presentation: 福井県立大学福井キャンパス
• [Presentation] 花の開花ステージにおけるα-アラビノフラノシダーゼ遺伝子の発現 (2012)
  • Author(s): 吉本圭吾、立石 亮、渡辺慶一、井上弘明
  • Organizer: 園芸学会
  • Place of Presentation: 福井県立大学
• [Presentation] Availability of BY-2 as a heterologous expression system to obtain active recombinant proteins (2011)
  • Author(s): Akira Tateishi
  • Organizer: 3rd Horticultural Physiology Workshop -International workshop on transgenic solanaceeus plants-.
  • Place of Presentation: Kobe Convention Center
  • Year and Date: 2011-11-29
• [Presentation] Availability of BY-2 as a heterologous expression system to obtain active recombinant proteins (2011)
  • Author(s): Akira Tateishi
  • Organizer: 3rd Horticultural Physiology Workshop-International workshop on transgenic solanaceous plants-
  • Place of Presentation: 兵庫(招待講演)
  • Year and Date: 2011-11-29
• [Presentation] Active tomato α-L-arabinofuranosidases expressed in a plant cultured cells and substrate specificity of the recombinant enzymes (2011)
  • Author(s): Tomohiro Nakajima, Akira Tateishi and Hiroaki Inoue
  • Organizer: 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference.
  • Place of Presentation: Kobe Convention Center
• [Presentation] Expression analysis and heterologous expression of α-L-arabinofuranosidase/β-xylosidases (2011)
  • Author(s): Akira Tateishi, Tomohiro Nakajima and Hiroaki Inoue
  • Organizer: The 4th Conference on Biosynthe-sis of Plant Cell Wall.
  • Place of Presentation: Awaji Yumebutai Interna-tional Conference Center
• [Presentation] 異種細胞系で発現させたトマトのα-アラビノフラノシダーゼ/β-キシロシダーゼにおける基質特異性の解析 (2011)
  • Author(s): 中島智広、立石亮、井上弘明
  • Organizer: 園芸学会
  • Place of Presentation: 岡山大学津島キヤンパス
• [Presentation] Expression analysis of glycoside hydrolase family 3 α-L-arabinofuranosidase/β-xylosidase in tomato Plant (2011)
  • Author(s): Tomohiro Nakajima, Akira Tateishi and Hiroaki Inoue
  • Organizer: Joint Internation-al Symposium on Japanese Solanaceae/Cucurbitaceae Genomics Initiatives.
  • Place of Presentation: 岡山大学
• [Presentation] 異種細胞系で発現させたトマトのα-アラビノフラノシダーゼ/β-キシロシダーゼにおける基質特異性の解析 (2011)
  • Author(s): 中島智広・立石亮・井上弘明
  • Organizer: 園芸学会
  • Place of Presentation: 岡山
• [Presentation] Expression analysis and heterologous expression of α-L-arabinofuranosidase/β-xylosidases (2011)
  • Author(s): Akira Tateishi, Tomohiro Nakajima, Hiroaki Inoue
  • Organizer: The 4th Conference on Biosynthesis of Plant Cell Wall
  • Place of Presentation: 兵庫
• [Presentation] Active tomato α-L-arabinofuranosidases expressed in a plant cultured cells and substrate specificity of the recombinant enzymes (2011)
  • Author(s): Tomohiro Nakajima, Akira Tateishi, Hiroaki Inoue
  • Organizer: 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference
  • Place of Presentation: 兵庫
• [Presentation] トマトからの新奇糖質加水分解酵素遺伝子のクローニングと発現解析 (2010)
  • Author(s): 高橋翔、立石亮、渡辺慶一、井上弘明
  • Organizer: 園芸学会
  • Place of Presentation: 大分大学旦野原キャンパス
• [Presentation] Changes in α-L-arabinofuranosidase activity and isolation of related clone from ripening avocado fruit (2010)
  • Author(s): Sho Takahashi, Akira Tateishi, Keiichi Watanabe, Kazunari Nomura, HirGaki Inoue
  • Organizer: 28th Interna- tional Horticultural Congress.
  • Place of Presentation: Lisbon Congress Center (Portugal)
• [Presentation] Cloning and expression analysis of α-L-arabinofuranosidase/β-xylosidase from tomato fruit' (2010)
  • Author(s): Akira Tateishi, Kaoru Okada, Sho Takahashi, Keiichi Watanabe, Kazunari Nomura, Hiroaki Inoue
  • Organizer: 28th International Horticultural Congress.
  • Place of Presentation: Lisbon Congress Center(Portugal)
• [Presentation] Changes in α-L-arabinofuranosidase activity and isolation of related clone from ripening avocado fruit (2010)
  • Author(s): Sho Takahashi, Akira Tateishi, Keiichi Watanabe, Kazunari Nomura, Hiroaki Inoue
  • Organizer: 28th International Horticultural Congress
  • Place of Presentation: ポルトガル
• [Presentation] Cloning and expression analysis of α-L-arabinofuranosidase/β-xylosidase from tomato fruit (2010)
  • Author(s): Akira Tateishi, Kaoru Okada, Sho Takahashi, Keiichi Watanabe, Kazunari Nomura, Hiroaki Inoue
  • Organizer: 28th International Horticultural Congress
  • Place of Presentation: ポルトガル
• [Presentation] トマトからの新奇糖質加水分解酵素遺伝子のクローニングと発現解析 (2010)
  • Author(s): 高橋翔、立石亮、渡辺慶一、井上弘明
  • Organizer: 園芸学会
  • Place of Presentation: 大分