Characterization of intervarietal crossing and establishment of base for breeding in mango.

Research Project

Project/Area Number	22580043
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Horticulture/Landscape architecture
Research Institution	Kinki University
Principal Investigator	KANZAKI Shinya 近畿大学, 農学部, 講師 (20330243)
Co-Investigator(Kenkyū-buntansha)	HONSHO Chitose 宮崎大学, 農学部, 助教 (30381057)
Project Period (FY)	2010 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	DNAマーカー / 果皮色 / 親子鑑定 / 自家不結実性 / 分子マーカー / 多胚性
Research Abstract	To develop an practical method for determining the male parent of mango seedlings, high-polymorphic SSR markers were selected and multiplex PCR method was developed. Progenies obtained from open pollinated 'Irwin' trees and 'Beni-Keitt' trees in a plastic house were used for determining male parents. The results showed that cross-pollinated seedlings were major in both cultivars. In addition, Myb transcription factor associated with anthocyanin biosynthesis were isolated from mango and its structure was analyzed.