Studies on the mechanisms of transcriptional regulation of Tobacco mosaic virus resistance gene and induction of hypersensitive response

• Project/Area Number: 22580046
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: NYUNOYA Hiroshi 東京農工大学, 学術研究支援総合センター, 教授 (60135936)
• Co-Investigator(Kenkyū-buntansha): SASAKI Nobumitsu 東京農工大学, 学術研究支援総合センター, 助教 (70431971)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: タバコモザイクウイルス / 抵抗性遺伝子 / Dof転写因子 / 過敏感応答 / N遺伝子 / 細胞死 / エリシター / 転写因子 / Dof / タバコ / ウイルス / 植物 / 植物病 / 転写活性化

Research Abstract

Dof transcription factors BBF1, BBF2 and BBF3 were binding factors to the region upstream of the N gene promoter. Transient expression of BBF1 led to enhancements of N gene transcription, ROS production and HR induction.

Research Products

• [Journal Article] Overexpression of a tobacco Dof transcription factor BBF1 stimulates the transcription of the tobacco mosaic virus resistance gene <i>N</i> and defense-related responses including ROS production (2013)
  • Author(s): Mayumi Takano, Md. Ashraful Haquea, Shota Odaira, Keiko Nakata, Nobumitsu Sasaki, and Hiroshi Nyunoya
  • Journal Title: Plant Biotechnology Volume: 30 Issue: 1 Pages: 37-46
  • DOI: 10.5511/plantbiotechnology.12.1207a
  • NAID: 10031163817
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] Identification of a movement protein of Mirafiori lettuce big-vein ophiovirus (2013)
  • Author(s): A Hiraguri
  • Journal Title: Journal of General Virology Volume: 94 Issue: 5 Pages: 1145-1150
  • DOI: 10.1099/vir.0.050005-0
  • Peer Reviewed
• [Journal Article] The movement protein encoded by gene 3 of rice transitory yellowing virus is associated with virus particles (2012)
  • Author(s): Hiraguri A, Hibino H, Hayashi T, Netsu O, Shimizu T, Uehara-Ichiki T, Omura T, Sasaki N, Nyunoya H, Sasaya T
  • Journal Title: Journal of General Virology Volume: 93(10) Issue: 10 Pages: 2290-2298
  • DOI: 10.1099/vir.0.044420-0
  • Peer Reviewed
• [Journal Article] Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris (2012)
  • Author(s): Nobumitsu Sasaki, Tatsuro Odawara, Shoko Nagai, Kazuma Yoshimura, Yasuhiko Matsushita, Hiroshi Nyunoya
  • Journal Title: Physiological and Molecular Plant Pathology Volume: 78 Pages: 38-44
  • DOI: 10.1016/j.pmpp.2012.01.003
  • Peer Reviewed
• [Journal Article] The nonstructural protein pC6 of rice grassy stunt virus trans-complements the cell-to-cell spread of a movement-defective tomato mosaic virus The nonstructural protein pC6 of rice grassy stunt virus trans-complements the cell-to-cell spread of a movement-defective tomato mosaic virus (2011)
  • Author(s): A. Hiraguri, O. Netsu, T. Shimizu, T. Uehara-Ichiki, T. Omura, N. Sasaki, H. Nyunoya, T. Sasaya
  • Journal Title: Archives of Virology Volume: 156(5) Issue: 5 Pages: 911-916
  • DOI: 10.1007/s00705-011-0939-6
  • Peer Reviewed
• [Journal Article] Determination of structural regions important for Ca2+ uptake activity in Arabidopsis MCA1 and MCA2 expressed in yeast (2011)
  • Author(s): Masataka Nakano, Kazuko Iida, Hiroshi Nyunoya, Hidetoshi Iida
  • Journal Title: Plant and Cell Physiology Volume: 52 Issue: 11 Pages: 1915-1930
  • DOI: 10.1093/pcp/pcr131
  • Peer Reviewed
• [Journal Article] The nonstructural protein pC6 of rice grassy stunt virus trans-complements the cell-to-cell spread of a movement-defective tomato mosaic virus (2011)
  • Author(s): Akihiro Hiraguri, et al.
  • Journal Title: Archives of Virology Volume: 156 Pages: 911-916
  • Peer Reviewed
• [Journal Article] Characterization and cloning of TMV resistance gene N homologues from Nicotiana tabacum (2010)
  • Author(s): J-S. Gao, Y. Meng, N. Sasaki, H. Kanegae, N. Hayashi, H. Nyunoya
  • Journal Title: African Journal of Biotechnology Volume: 9(47) Issue: 47 Pages: 7998-8006
  • DOI: 10.5897/ajb10.732
  • Peer Reviewed
• [Journal Article] Characterization and cloning of TMV resistance gene N homologues from Nicotiana tabacum (2010)
  • Author(s): Jun-Shan Gao, et al.
  • Journal Title: African Journal of Biotechnology Volume: 9 Pages: 7998-8006
  • Peer Reviewed
• [Presentation] Involevment of Dof proteins in the Transcriptional Activation of the Tobacco Resistance Gene N by Tobacco Mosaic Virus elicitor and Its Implication for the induction of Hypersensitive Response (2013)
  • Author(s): H. Nyunoya, M. Takano, S. Odaira, Md. A. Haque, N. Sasaki
  • Organizer: BIT's 2nd Annual World Congress of Microbes-2012
  • Place of Presentation: 広州市(中国)
  • Year and Date: 2013-08-01
• [Presentation] Overexpression of tobaco Dof transcription factor enhances transcriptional activation of the virus resistance gene N and ROS generation (2013)
  • Author(s): M Takano, MA Haque, N Sasaki, H Nyunoya
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都市
  • Year and Date: 2013-07-30
• [Presentation] The role of a splice variant product of the virus resistance gene N in the induction of hypersensitive response (2013)
  • Author(s): M Takaoka, M Takano, MA Haque, N Sasaki, H Nyunoya
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都市
  • Year and Date: 2013-07-30
• [Presentation] OVEREXPRESSION OF THE DOF PROTEIN IN TOBACCO LEADS TO TRANSCRIPTIONAL ACTIVATION OF THE RESISTANCE GENE N, SUGGESTING POTENTIAL ROLES IN THE REGULATION OF HYPERSENSITIVE RESPONSE TO TMV (2013)
  • Author(s): Takano M., Odaira S.1, Haque M.A. Sasaki N. and Nyunoya H
  • Organizer: The 24th International Conference on Arabidopsis Research
  • Place of Presentation: シドニー(オーストラ リア)
  • Year and Date: 2013-06-25
• [Presentation] タバコDofタンパク質BBF1の一過的過剰発現時における防御関連遺伝子の発現解析 (2013)
  • Author(s): 高野真由美、アシュラフル・ハーク、佐々木信光、丹生谷博
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2013-03-29
• [Presentation] 抵抗性因子Nのスプライシングバリアントの一過的過剰発現はエリシター誘導性の過敏感細胞死およびウイルス抵抗性を抑制する (2013)
  • Author(s): 高岡万純,佐々木信光,丹生谷博
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2013-03-29
• [Presentation] Transcriptional activation of the N gene and increase of ROS generation by transient overexpression of tobacco Dof protein BBF1 (2013)
  • Author(s): M. Takano, Md. A. Haque, N. Sasaki, H. Nyunoya
  • Organizer: The 2nd Korea-Japan Joint symposium
  • Place of Presentation: 福岡市
  • Year and Date: 2013-03-27
• [Presentation] THE P3 PROTEIN ENCODED BY THE THIRD GENE OF LETTUCE BIG-VEIN ASSOCIATED VARICOSAVIRUS RNA2 IS A VIRAL CELL-TO-CELL MOVEMENT PROTEIN (2013)
  • Author(s): T Sasaya
  • Organizer: IXth Symposium of International Working Group of Plant Viruses with Fungal Vectors
  • Place of Presentation: 帯広市
• [Presentation] OVEREXPRESSION OF THE DOF PROTEIN IN TOBACCO LEADS TO TRANSCRIPTIONAL ACTIVATION OF THE RESISTANCE GENE N, SUGGESTING POTENTIAL ROLES IN THE REGULATION OF HYPERSENSITIVE RESPONSE TO TMV (2013)
  • Author(s): M Takano
  • Organizer: The 24th International Conference on Arabidopsis Research
  • Place of Presentation: Sydney, Australia
• [Presentation] Transcriptional activation of the N gene and increase of ROS generation by transient overexpress ion of tobacco Dof protein BBF1 (2012)
  • Author(s): Takano M., Hague Md.A., Sasaki, N., Nyunoya, N.
  • Organizer: The 2nd Korea-Japan Joint Symposium(日韓植物病理学会合同大会)
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-03-27
• [Presentation] N遺伝子による防御応答におけるタバコDofタンパク質BBF2の機能解析 (2012)
  • Author(s): 小平将太,松丸昌道,仲田積実,佐々木信光,丹生谷博
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都市
  • Year and Date: 2012-03-16
• [Presentation] N遺伝子による防御応答におけるタバコDofタンパク質BBF2の機能解析 (2012)
  • Author(s): 小平将太, 松丸昌道, 仲田積実, 佐々木信光, 丹生谷博
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都市)
  • Year and Date: 2012-03-16
• [Presentation] TMV抵抗性遺伝子Nのスプライスバリアント産物のHR誘導における役割 (2011)
  • Author(s): 高岡万純,高野真由美,ハークアシュラフル,佐々木信光,丹生谷博
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-12-15
• [Presentation] ウイルス抵抗性遺伝子Nを介した過敏感応答の誘導におけるタバコDofタンパク質BBF1の機能解析 (2011)
  • Author(s): 高野真由美,ハークアシュラフル,佐々木信光,丹生谷博
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-12-15
• [Presentation] ウイルス抵抗性遺伝子Nを介した過敏感応等の誘導におけるタバコDofタンパク質BBF1の機能解析 (2011)
  • Author(s): 高野真由美, Hague, Md.A, 佐々木信光, 丹生谷博
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-13
• [Presentation] OverexpressionofaDof transcription factor accelerates hypersensitive response to the elicitor of Tobacco mosaic virus through the upregulation of the tobacco resistance gene N (2011)
  • Author(s): M. Takano, S. Odaira, Md. A. Haque, N. Sasaki,H.Nyunoya
  • Organizer: 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference
  • Place of Presentation: 神戸市
  • Year and Date: 2011-11-29
• [Presentation] Virus resistance of transgenic tobacco plants expressing a movement protein-binding protein (2011)
  • Author(s): N. Sasaki,T. Odawara, H. Nyunoya
  • Organizer: 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference
  • Place of Presentation: 神戸市
  • Year and Date: 2011-11-28
• [Presentation] Overexpression of a Dof transcription factor accelerates hypersensitive response to the elicitor of Tobacco mosaic virus through the upregulati on of the tobacco resistance gene N (2011)
  • Author(s): Takano, M., Odaira, S., Haque, Md.A., Sasaki, N, Nyunoya, N
  • Organizer: 8th Solanaceae and cucurbitaceae genome joint conference
  • Place of Presentation: 神戸コンベンションセンター(神戸市)
  • Year and Date: 2011-11-28
• [Presentation] Interfered cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants over-expressing BcKELP, a binding factor for viral movement proteins (2011)
  • Author(s): N Sasaki, T Odawara, H Nyunoya
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-11
• [Presentation] Transcriptional Transactivation of the Tobacco Resistance Gene N for Tobacco mosaic virus through its Own Product N Protein (2011)
  • Author(s): H. Nyunoya, N. Sasaki, Md. A. Haque, M. Takano, S. Odaira
  • Organizer: BIT's 1st Annual World Congress of Microbes-2011
  • Place of Presentation: 北京市(中国)
  • Year and Date: 2011-08-01
• [Presentation] Transcriptional transactivation of the tobacco resistance gene N for Tobacco mosaic virus through its own product N protein (2011)
  • Author(s): Nyunoya, H., Sasaki, N., Haque, Md.A., Takano, M., Odaira, S.
  • Organizer: BIT'S 1st Annual World Congress of Microbes
  • Place of Presentation: 北京国際会議センター(中国)(招待講演)
  • Year and Date: 2011-07-30
• [Presentation] ウイルス抵抗性遺伝子Nのエリシター応答配列におけるタバコDofタンパク質BBF1の認識配列の解析 (2011)
  • Author(s): 小平将太,アシュラフルハーク,佐々木信光,丹生谷博
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 府中市
  • Year and Date: 2011-03-27
• [Presentation] タバコDofタンパク質BBF1の一過的過剰発現はタバコモザイクウイルス抵抗性遺伝子Nのプロモーターの活性化をもたらす (2011)
  • Author(s): 高野真由美,アシュラフルハーク,佐々木信光,丹生谷博
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 府中市
  • Year and Date: 2011-03-27
• [Presentation] タバコDofタンパク質BBF1の-過的過剰発現はタバコモザイクウイルス抵抗性遺伝子Nのプロモーターの活性化をもたらす (2011)
  • Author(s): 高野真由美, ほか
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 東京農工大学(東京都)
  • Year and Date: 2011-03-27
• [Presentation] ウイルス抵抗性遺伝子Nのエリシター応答配列におけるタバコDofタンパク質BBF1の認識配列の解析 (2011)
  • Author(s): 小平将太, ほか
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 東京農工大学(東京都)
  • Year and Date: 2011-03-27
• [Presentation] タバコのDofタンパク質BBF1の-過的発現はウイルス抵抗性囎伝子のプロモーター活性を増強する (2010)
  • Author(s): 高野真由美, ほか
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] タバコDofタンパク質BBF1と抵抗性遺伝子Nのウイルスエリシター応答配列との結合解析 (2010)
  • Author(s): 小平将太, ほか
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] Tobacco transcription factor Dof proteins mediate the promoter activation of the resistance gene N against Tobacco mosaic virus (2010)
  • Author(s): N. Sasaki, Md. A. Haque, M. Takano, S. Odaira, H. Nyunoya
  • Organizer: Cold Spring Harbor Asia Conference-From Plant Biology to Crop Biotechnology
  • Place of Presentation: 蘇州市(中国)
  • Year and Date: 2010-10-26
• [Presentation] Tobacco transcription factor Dof proteins mediate the promoter activation of the resistance gene Nagainst Tobacco mosaic virus (2010)
  • Author(s): Nobumitsu Sasaki, et al
  • Organizer: Cold Spring Harbor Asia Conference From Plant Biology to Crop Biotechnology
  • Place of Presentation: Cold Spring Harbor Asia, Suzhou, China
  • Year and Date: 2010-10-26
• [Presentation] Involvement of tobacco transcription factor Dof proteins in the promoter activation of the resistance gene N against Tobacco mosaic virus (2010)
  • Author(s): N. Sasaki, M.A. Haque, M. Matsumaru, S. Odaira, H. Nyunoya
  • Organizer: International Congress of Arabidopsis Research 2010
  • Place of Presentation: 横浜市
  • Year and Date: 2010-06-07
• [Presentation] Involvement of tobacco transcription factor Dof proteins in the promoter activation of the resistance gene Nagainst Tobacco mosaic virus (2010)
  • Author(s): Nobumitsu Sasaki et a1.
  • Organizer: International Congress of Arabidopsis Research 2010
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2010-06-07
• [Presentation] タバコDofタンパク質BBF1の一過的過剰発現時における防御関連遺伝子の発現解析
  • Author(s): 高野真由美
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜市
• [Presentation] 抵抗性因子Nのスプライシングバリアントの一過的過剰発現はエリシター誘導性の過敏感細胞死およびウイルス抵抗性を抑制する
  • Author(s): 高岡万純
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜市
• [Presentation] Involevment of Dof proteins in the Transcriptional Activation of the Tobacco Resistance Gene N by Tobacco Mosaic Virus elicitor and Its Implication for the induction of Hypersensitive Response
  • Author(s): H Nyunoya
  • Organizer: BIT's 2nd Annual World Congress of Microbes-2012
  • Place of Presentation: 広州市，中国
• [Presentation] Overexpression of tobaco Dof transcription factor enhances transcriptional activation of the virus resistance gene N and ROS generation
  • Author(s): M Takano
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions (IS-MPMI 2012)
  • Place of Presentation: 京都市
• [Presentation] The role of a splice variant product of the virus resistance gene N in the induction of hypersensitive response
  • Author(s): M Takaoka
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions (IS-MPMI 2012)
  • Place of Presentation: 京都市
• [Remarks]
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• [Remarks]
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