| Project/Area Number |
22580048
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Kobe University |
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Principal Investigator |
朴 杓允 神戸大学, 大学院・農学研究科, 名誉教授 (20147094)
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Kenichi 神戸大学, 大学院・農学研究科, 准教授 (40437504)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 酸素バブリング / 感染応答タンパク / 糖タンパク / グルタルアルデヒド / 微細構造 / 冠根 / トルイジン青 / 液胞 / 酸素バブリング固定 / グルタルアルデヒド固定 / 根冠 / 感染応答蛋白質 / 糖蛋白質 / トルイジン |
|---|
| Research Abstract |
A new chemical fixation method was developed successfully in this project. On fixing plants with glutaraldehyde together with oxygen bubbling, the higher preservation of glycoproteins was achieved, resulting in observing fine ultrastructure under electron microscope. The cell images involving membranes and matrix of organelles, cytoplasm and cell components were excellent. However, it was not yet apparent whether the bubbling fixation was good enough to the preservation of protein for immunoelectron microscopy or not.
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