Genetic analysis of the haploid strain in the potato late blight pathogen Phytophthora infestans

• Project/Area Number: 22580050
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Okayama University
• Principal Investigator: TAGA Masatoki 岡山大学, 大学院・自然科学研究科, 教授 (80236372)
• Co-Investigator(Renkei-kenkyūsha): AKINO Seishi 北海道大学, 大学院・農学研究院, 講師 (60202537)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 疫病菌 / ジャガイモ・ゲノム / 倍数性 / Phytophthora / 遺伝学 / 半数体 / 染色体 / ゲノム / 形質転換 / nit変異 / ジャガイモ

Research Abstract

Although the potato late blight pathogen, Phytophthora infestans, has long been believed to be diploid or of higher level of ploidy, we discovered a haploid isolate recently. In this study, we carried out genetic analyses on this isolate, including (1)molecular phylogenetic analysis based on nuclear and mitochondrial genes; (2) flow cytometric analysis of nuclear DNA content and stability of genome; (3)microscopic observation on the somatic nuclei and chromosomes; (4)growth characteristics; (5)production of auxotrophic mutants; (6)establishment of transformation method.