Role of stress response signal transduction pathway in trichothecene production in Fusarium graminearum

• Project/Area Number: 22580054
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Toyo University
• Principal Investigator: FUJIMURA Makoto 東洋大学, 生命科学部, 教授 (50297735)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 赤かび病 / MAPキナーゼ / cAMP / アカパンカビ / 麦類赤かび病 / 殺菌剤 / トリコテセン / ストレス応答

Research Abstract

The deletion mutants of FgATF1 gene which encodes a putative transcription factor regulated by stress response MAP kinase FgOS2 were isolated and characterized in Fusarium graminearum. Δ FgATF1 strain lack the ability to produce trichothecenes, whereas FgATF1 deletion in an aconidial strain did not affect trichothecene production. These results suggest that FgATF1 indirectly regulates the trichothecene synthesis probably through asexual differentiation. Furthermore we identified 11 genes which are regulated under the stress response MAP kinase pathway, they included various genes such as glycerol dehydrogenase, conidial catalase, clock-controlled genes, gluconeogenesis key enzymes, and cell wall enzymes.

Research Products

• [Journal Article] Regulation and physiological function of MAP kinase and cAMP-PKA pathways In: Neurospora: Genomics and Molecular Biology. (2013)
  • Author(s): Masayuki Kamei, Masakazu Takahashi, Shinpei Banno, Akihiko Ichiishi, Fumiyasu Fukumori, Makoto Fujimura (2013)
  • Journal Title: D. Kasbekar & K. McCluskey (eds). Pages: 171-192
  • Peer Reviewed
• [Journal Article] Deletion and expression analysis of beta-(1,3)-glucanosyltransferase genes in Neurospora crassa (2013)
  • Author(s): Masayuki Kamei, Kazuhiro Yamashita, Masakazu Takahashi, Fumiyasu Fukumori, Akihiko Ichiishi, Makoto Fujimura
  • Journal Title: Fungal Genetics and Biology Volume: 52 Pages: 62-75
  • DOI: 10.1016/j.fgb.2012.12.001
  • Peer Reviewed
• [Journal Article] ストレス応答シグナル伝達経路に作用する殺菌剤に関する研究 (2010)
  • Author(s): 藤村真
  • Journal Title: 日本農薬学会誌 Volume: 35 Pages: 363-369
  • NAID: 110007681558
• [Presentation] 赤かび病菌におけるアカパンカビ薬剤誘導遺伝子の発現解析 (2011)
  • Author(s): 齋須秀昭, 高橋正和, 亀井誠之, 藤村真
  • Organizer: 日本農芸化学会関東支部2011年度大会
  • Place of Presentation: 東洋大学(群馬県)
  • Year and Date: 2011-10-15
• [Presentation] 糸状菌のシグナル伝達経路の薬剤応答と機能分化 (2011)
  • Author(s): 藤村真
  • Organizer: 第15回農薬相模セミナー
  • Place of Presentation: (公財)相模中央化学研究所(神奈川県)
  • Year and Date: 2011-01-06
• [Presentation] アカパンカビのストレス応答MAPキナーゼ下流で制御される転写因子RCO-1の同定 (2010)
  • Author(s): 山下和宏、高橋正和、亀井誠之、藤村真
  • Organizer: 第10回糸状菌分子生物学コンファレンス
  • Place of Presentation: 広島大学(広島県)
  • Year and Date: 2010-11-19
• [Presentation] 赤かび病菌のCREB型転写因子FgATF1破壊株の単離とその解析 (2010)
  • Author(s): 齋須秀昭、山下和宏、高橋正和、亀井誠之、木村真、藤村真
  • Organizer: 第10回糸状菌分子生物学コンファレンス
  • Place of Presentation: 広島大学(広島県)
  • Year and Date: 2010-11-18
• [Presentation] Comparative transcriptional profiling of Neurospora crassa and Fusarium graminearum in response to fungicides.
  • Author(s): Makoto Fujimura, Kazuhiro Yamashita, Masakazu Takahashi, Masayuki Kamei, Moto Miyashita
  • Organizer: 5th Pan-Pacific Conference of Pesticide Science
  • Place of Presentation: Beijing, China
  • Invited
• [Presentation] Fluazinam induced the glutathione S-transferase genes and oxidoreductases gene in transcription factor NAP-1-dependent and -independent manner in Neurospora crassa
  • Author(s): Masakazu Takahashi, Shinpei Banno, Makoto Fujimura,
  • Organizer: 5th Pan-Pacific Conference of Pesticide Science
  • Place of Presentation: Beijing, China
• [Presentation] Hybridization probe and Pyrosequencing assays to monitor fungicide-resistant population for the cucumber pathogens
  • Author(s): Shinpei Banno, Tsuyoshi Shinpuku, Yohei Ishigami, Masakazu Takahashi, Makoto Fujimura
  • Organizer: 5th Pan-Pacific Conference of Pesticide Science
  • Place of Presentation: Marburg, Germany
• [Presentation] Contribution of MAK-1 and MAK-2 MAP kinases to cell wall integrity in Neurospora crassa
  • Author(s): Masayuki Kamei, Masakazu Takahashi, Akihiko Ichiishi, Makoto Fujimura
  • Organizer: 11th Europium Conference of Fungal Genetics
  • Place of Presentation: Marburg, Germany
• [Presentation] Deletion of cAMP phosphodiesterase pde-2/acon-2 gene causes the enhanced osmotic sensitivity in os-1 and os-2 mutants of N. crassa
  • Author(s): Chika Kurata, Masayuki Kamei, Setsuko Watanabe, Shinpei Banno, Makoto Fujimura
  • Organizer: 27th Fungal Genetics Conference
  • Place of Presentation: California, USA
• [Presentation] Neurospora crassaのエルゴステロール生合成阻害剤によるerg遺伝子の誘導とその制御
  • Author(s): 宮下 基，亀井誠之，高橋正和，一石昭彦，藤村 真
  • Organizer: 第12回糸状菌分子生物学コンファレンス
  • Place of Presentation: ウインクあいち(名古屋）
• [Presentation] アカパンカビOS-2 MAPキナーゼによる細胞壁局在タンパク質の制御
  • Author(s): 加賀谷奏，山下和宏，高橋正和，亀井誠之，一石昭彦，藤村 真
  • Organizer: 第12回糸状菌分子生物学コンファレンス
  • Place of Presentation: ウインクあいち(名古屋）
• [Presentation] フルアジナムにより誘導されるアカパンカビの遺伝子群の同定
  • Author(s): 高橋正和，亀井誠之，宮下 基，福森文康，藤村 真
  • Organizer: 第12回糸状菌分子生物学コンファレンス
  • Place of Presentation: ウインクあいち(名古屋）
• [Book] Horizon Scientific Press and Caister Academic Press (2013)
  • Author(s): Masayuki Kamei, Masakazu Takahashi, Shinpei Banno, Akihiko Ichiishi, Fumiyasu Fukumori, Makoto Fujimura
  • Publisher: Neurospora: Genomics and Molecular Biology
• [Book] Regulation and physiological function of MAP kinase and cAMP-PKA pathways. In : D. Kasbekar. K. McCluskey, Eds.), Neurospora : Genomics and Molecular Biology (2013)
  • Author(s): Kamei M., Banno S., Takahashi M., Ichiishi A., Fukumori F., Fujimur, M.
  • Publisher: Horizon Scientific Press and Caister Academic Press, Norwich, UK(掲載決定済)