Development of new BT agents and Coleoptera adult insecticidal activity mechanism

• Project/Area Number: 22580056
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Hokkaido University
• Principal Investigator: ASANO Shinichiro 北海道大学, 大学院・農学研究院, 准教授 (60222585)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 害虫管理 / 生物的防除 / galleriae SDS-502 / Cry8Daトキシン / レセプター / 作用機構 / βグルコシデース / 成虫殺虫活性 / アフィニティークロマト / 中腸上皮組織 / Bacillus thuringiensis / cry8トキシン / 鞘翅目害虫 / ハムシ類 / コガネムシ類 / 腸管消化液 / トキシン受容体 / 防除法 / cry8 トキシン

Research Abstract

To find the novel Cry toxin with specific insecticidal activity in adult Coleoptera pests, we were screening by PCR and Bioassay from our B. thuringiensis strains’ library. As microbial control agent against Coleoptera adults to conduct for the scarab beetle adult and leaf beetles adult of Cry8D toxin, and to elucidate the insecticidal activity mechanism adult-specific, is a flame pest control, I develop a new BT agents.

Research Products

• [Journal Article] Intramolecular cleavage at the loop between α3-helix and α4-helix is critical for cytotoxic activity of Cry8Da (2013)
  • Author(s): T. Yamaguchi, H. Bando, S. Asano
  • Journal Title: IOBC-wprs Bulletin
  • Peer Reviewed
• [Journal Article] Identification of a Bacillus thuringiensis Cry8Da toxin-binding glucosidase from the adult Japanese beetle, Popillia japonica (2013)
  • Author(s): T. Yamaguchi, H. Bando, S. Asano
  • Journal Title: J. Invertebrate Pathology Volume: Vol.113 Issue: 2 Pages: 123-128
  • DOI: 10.1016/j.jip.2013.03.006
  • Peer Reviewed
• [Journal Article] Fifty kDa Activated Cry2Aa Bacillus thuringiensis Toxin Killed Larvae of Bombyx mori and Lymantria dispar (Asian Type) (2012)
  • Author(s): M. Ohsawa, M. Tanaka, K. Moriyama, M. Shimazu, S. Asano, K. Miyamoto, K. Haginoya, T. Mitsui, T. Kohya, M. Taniguchi, and H. Hori
  • Journal Title: Appl. Environ. Microbiol Volume: Vol.78(13) Issue: 13 Pages: 4755-4757
  • DOI: 10.1128/aem.07123-11
  • Peer Reviewed
• [Journal Article] ネッタイシマカアミノペプチデースNのCry11Aaトキシンレセプターとしての機能について (2011)
  • Author(s): 小池遼・佐原健・伴戸久徳・浅野眞一郎
  • Journal Title: 東北蚕糸・昆虫利用研究報告 Volume: 36巻
• [Journal Article] Cry39AaトキシンのドメインIIループ1がハマダラカ殺虫活性に与える影響 (2011)
  • Author(s): 石垣俊一郎・佐原健・伴戸久徳・浅野眞一郎
  • Journal Title: 東北蚕糸・昆虫利用研究報告 Volume: 36巻
• [Journal Article] 鞘翅目昆虫におけるCry8Daトキシンレセプター分子の探索 (2011)
  • Author(s): 竹林健人・佐原健・伴戸久徳・浅野眞一郎
  • Journal Title: 東北蚕糸・昆虫利用研究報告 Volume: 35巻
• [Journal Article] Possibility of insect pathogenic microbe as hygiene insect pest control materials (2011)
  • Author(s): 浅野眞一郎
  • Journal Title: Journal of Pesticide Science Volume: 36 Issue: 1 Pages: 91-93
  • DOI: 10.1584/jpestics.W10-73
  • NAID: 110008463017
  • ISSN: 1348-589X, 1349-0923
  • Peer Reviewed
• [Journal Article] 鞘翅目昆虫におけるCry8Daトキシンレセプター分子の探索 (2011)
  • Author(s): 竹林健人, ら
  • Journal Title: 東北蚕糸・昆虫利用研究報告 Volume: 35 Pages: 3-3
• [Journal Article] Cry39AaトキシンのドメインIIループ1がハマダラカ殺虫活性に与える影響 (2011)
  • Author(s): 石垣俊一郎, ら
  • Journal Title: 東北蚕糸・昆虫利用研究報告 Volume: 36 Pages: 14-14
• [Journal Article] ネッタイシマカアミノペプチデースNのCry11Aaトキシンレセプターとしての機能について (2011)
  • Author(s): 小池遼, ら
  • Journal Title: 東北蚕糸・昆虫利用研究報告 Volume: 36 Pages: 15-15
• [Journal Article] Intramolecular proteolytic nicking and binding of Bacillus thuringiensis Cry8Da toxin in BBMVs of Japanese beetle (2010)
  • Author(s): T. Yamaguchi, K. Sahara, H. Bando and S.Asano
  • Journal Title: J. Invertebrate Pathology Volume: Vol.105 Issue: 3 Pages: 257-262
  • DOI: 10.1016/j.jip.2010.07.002
  • Peer Reviewed
• [Journal Article] Characterization of cry8Da genes isolated from Bacillus thuringiensis strains in Vietnam (2010)
  • Author(s): M. L. T. Thanh, P. K. Thuy, S. Asano, H. Hori, D. Dean and N. D. Binh
  • Journal Title: Proceeding THE 3RD INTERNATIONAL MEETING FOR THE DEVELOPMENT OF INTEGRATED PEST MANAGEMENT (IPM) IN ASIA AND AFRICA Volume: Vol.3 Pages: 186-192
• [Journal Article] Microbial control of beetle in Japan (2010)
  • Author(s): S.Asano, T. Yamaguchi, H. Bando and K.Takeuchi
  • Journal Title: Proceeding THE 3RD INTERNATIONAL MEETING FOR THE DEVELOPMENT OF INTEGRATED PEST MANAGEMENT (IPM) IN ASIA AND AFRICA Volume: Vol.3 Pages: 49-52
• [Journal Article] Microbial control of beetle in Japan (2010)
  • Author(s): S.Asano, et al
  • Journal Title: THE 3^<RD> INTERNATIONAL MEETING FOR THE DEVELOPMENT OF INTEGRATED PEST MANAGEMENT (IPM) IN ASIA AND AFRICA Volume: 3 Pages: 49-52
• [Journal Article] Characterization of cry8Da genes isolated from Bacillus thuringiensis strains in Vietnam (2010)
  • Author(s): Binh, et al
  • Journal Title: THE 3^<RD> INTERNATIONAL MEETING FOR THE DEVELOPMENT OF INTEGRATED PEST MANAGEMENT (IPM) IN ASIA AND AFRICA Volume: 3 Pages: 186-192
• [Journal Article] Intramolecular proteolytic nicking and binding of Bacillus thuringiensis Cry8Da toxin in BBMVs of Japanese beetle (2010)
  • Author(s): T.Yamaguchi, et al
  • Journal Title: J.Invertebrate Pathology Volume: 105 Pages: 243-247
  • Peer Reviewed
• [Presentation] Cry8Dトキシンの鞘翅目成虫活性について (2012)
  • Author(s): 浅野 眞一郎
  • Organizer: 昆虫病理研究会シンポジウム
  • Place of Presentation: 帯広畜産大学（帯広）
  • Invited
• [Presentation] コクヌストモドキ由来A Disintegrin And Metalloprotease(ADAM)について (2012)
  • Author(s): 半谷 大樹
  • Organizer: 昆虫病理研究会シンポジウム
  • Place of Presentation: 帯広畜産大学（帯広）
• [Presentation] 鞘翅目昆虫に対する殺虫活性機構について (2011)
  • Author(s): 浅野眞一郎
  • Organizer: 第84回 日本生化学会大会
  • Place of Presentation: 京都国際会館(京都市)(招待講演)
  • Year and Date: 2011-09-22
• [Presentation] Activation process of Coleopteracidal Cry8 on alder leef beetle (2011)
  • Author(s): Asano, et al
  • Organizer: 44^<th> Annual Meeting of the Society for Invertebrate Pathology
  • Place of Presentation: St.Mary's University (Canada)
  • Year and Date: 2011-08-08
• [Presentation] The cleavage of the loop between alpha-3 and alpha-4 helix is critical for insecticidal activity of Cry8Da (2011)
  • Author(s): Yamaguchi, et al
  • Organizer: 44^<th> Annual Meeting of the Society for Invertebrate Pathology
  • Place of Presentation: St.Mary's University (Canada)
  • Year and Date: 2011-08-08
• [Presentation] Genetically Modifies Organism (2010)
  • Author(s): Shin-ichiro ASANO
  • Organizer: Sriwijaya University and Hokkaido University Joint Meeting
  • Place of Presentation: Palembang, Indonesia(招待講演)
  • Year and Date: 2010-12-08
• [Presentation] B.thuringiensis92-10株が産生するParasporin-1Aaについて (2010)
  • Author(s): 浅野眞一郎, ら
  • Organizer: 第4回パラスポリン研究会
  • Place of Presentation: 岡山市,岡山大学
  • Year and Date: 2010-10-22
• [Presentation] Cry39AaとCry44Aaの蚊類に対する特異的殺虫活性について (2010)
  • Author(s): 浅野眞一郎, ら
  • Organizer: 第16回BT研究会シンポジウム
  • Place of Presentation: 富士吉田市,山梨県
  • Year and Date: 2010-09-22
• [Presentation] Activation process of the coleopterancidal Cry8D on leaf-beetles (2010)
  • Author(s): Yamada, et al
  • Organizer: 43^<rd> Annual Meeting of the Society for Invertebrate Pathology
  • Place of Presentation: Trabzon, Turky
  • Year and Date: 2010-07-12
• [Presentation] Intramolecular proteolytic nicking and binding of Bacillus thuringiensis Cry8Da toxin in BBMVs of Japanese beetle (2010)
  • Author(s): Yamaguchi, et al
  • Organizer: 43^<rd> Annual Meeting of the Society for Invertebrate Pathology
  • Place of Presentation: Trabzon, Turky
  • Year and Date: 2010-07-12
• [Presentation] 虫害・線虫害防除の新展開、衛生害虫防除資材としての昆虫病原微生物の可能性 (2010)
  • Author(s): 浅野眞一郎
  • Organizer: 第35回日本農薬学会
  • Place of Presentation: 札幌市、北海道大学(招待講演)
  • Year and Date: 2010-05-29
• [Presentation] B.thuringiensis 92-10株が産生するParasporin-1Aa (92)の作用機構解析 (2010)
  • Author(s): 上森翔太, ら
  • Organizer: 第80回日本蚕糸学会
  • Place of Presentation: 上田市,信州大学
  • Year and Date: 2010-04-03
• [Remarks] Bacillus thuringiensis (BT)のライブラリー
  • URL: http://hashi.agr.hokudai.ac.jp/BT%20List.html