Studies on the overexpression of insect cytochrome P450s for the molecular identification of insecticide resistance

• Project/Area Number: 22580064
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: TOMITA Takashi 国立感染症研究所, 昆虫医科学部, 室長 (20180169)
• Co-Investigator(Kenkyū-buntansha): KASAI Shinji 国立感染症研究所, 昆虫医科学部, 主任研究官 (80332360)
• Co-Investigator(Renkei-kenkyūsha): KOMAGATA Osamu 国立感染症研究所, 昆虫医科学部, 研究員 (20435712) ; ITOKAWA Kentaro 国立感染症研究所, 昆虫医科学部, 流動研究員
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 害虫管理 / 生物的防除 / 殺虫剤抵抗性 / シトクロムP450 / マイクロアレイ / ネッタイイエカ / ピレスロイド / シス作用性突然変異 / 疾病媒介蚊 / 昆虫 / 遺伝学 / ゲノム / ピレスロイド系殺虫剤 / 遺伝子重複

Research Abstract

Cytochrome P450 genes genetically overexpressing and associated with each of larval and adult pyrethroid resistance in Culex quinquefasciatus were studied. We demonstrated that the overexpression of CYP9M10 due to cis-acting mutations is globally addressed to at least larval metabolic resistance to pyrethroids in this species and established a molecular genotyping method for identifying overexpressing CYP9M10 haplotypes.

Research Products

• [Journal Article] Global spread and genetic variants of the two CYP9M10 haplotype forms associated with insecticide resistance in Culex quinquefasciatus Say. (2013)
  • Author(s): Itokawa, K., Komagata, O., Kasai, S., Kawada, H., Mwatele, C., Dida, G., Njenga, S., Mwandawiro, C., Tomita, T
  • Journal Title: Heredity Volume: Epub ahead of print Issue: 3 Pages: 216-226
  • DOI: 10.1038/hdy.2013.40
  • Peer Reviewed
• [Journal Article] Cis-acting mutation and duplication: history of molecular evolution in a P450 haplotype responsible for insecticide resistance in Culex quinquefasciatus. (2011)
  • Author(s): Itokawa, K., Komagata, O., Kasai, S., Masada, M., Tomita, T
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 41 Issue: 7 Pages: 503-512
  • DOI: 10.1016/j.ibmb.2011.04.002
  • Peer Reviewed
• [Journal Article] 殺虫剤抵抗性蚊におけるシトクロムP450遺伝子の過剰発現 (2010)
  • Author(s): 冨田隆史, 糸川健太郎, 駒形修, 葛西真治
  • Journal Title: 日本農薬学会誌 Volume: 35 Pages: 562-568
  • NAID: 110008460402
  • Peer Reviewed
• [Presentation] 害虫の殺虫剤抵抗性機構 (2013)
  • Author(s): 冨田隆史
  • Organizer: 第155回日本獣医学会学術集会/日本比較薬理・毒性学会 企画シンポジウム「薬剤耐性の分子メカニズム」
  • Place of Presentation: 東京大学駒場キャンパス
• [Presentation] 殺虫剤抵抗性CYP9M10ハプロタイプの分子進化と拡散 (2012)
  • Author(s): 糸川健太郎, 駒形修, 葛西真治, 川田均, 冨田隆史
  • Organizer: 第56回日本応用動物昆虫学会大会
  • Place of Presentation: 近畿大学奈良キャンパス(奈良市)
  • Year and Date: 2012-03-29
• [Presentation] Spread of overexpressing CYP9M10 (cytochrome P450) haplotypes in southern house mosquito and its possible implication for vector control (2012)
  • Author(s): Itokawa, K., Komagata, O., Kawada, H., Tomita, T
  • Organizer: 24th International Conference of Entomology
  • Place of Presentation: Daegu, Korea
• [Presentation] Genetic variability of Culex Cyp9m10 and insecticide susceptibility (2012)
  • Author(s): Itokawa, K., Komagata, O., Kasai, S., Tomita, T
  • Organizer: 11th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
  • Place of Presentation: Torino, Italy
• [Presentation] 殺虫剤抵抗性CYP9M10 ハプロタイプの分子進化と拡散 (2012)
  • Author(s): 糸川健太郎, 駒形修, 葛西真治, 川田均, 冨田隆史
  • Organizer: 第56回日本応用動物昆虫学会大会
  • Place of Presentation: 近畿大学奈良キャンパス
• [Presentation] Genetic variability of Culex Cyp9m10 and insecticide susceptibility (2012)
  • Author(s): Takashi Tomita
  • Organizer: 11th International Symposium on Cytochrome P450 Biodiversity & Biotechnology
  • Place of Presentation: Torino, Italy
  • Invited
• [Presentation] ネッタイイエカCYPgMld.遺伝子の分子進化とピレスロイド抵抗性 (2011)
  • Author(s): 糸川健太郎, 駒形修, 葛西真治, 川田均, 岡村佳香, 政田正弘, 冨田隆史
  • Organizer: 第55回日本応用動物昆虫学会大会
  • Place of Presentation: (大会中止により講演要旨集上の発表となった)
  • Year and Date: 2011-03-22
• [Presentation] ネッタイイエカCYP9M10 遺伝子の分子進化とピレスロイド抵抗性 (2011)
  • Author(s): 糸川健太郎, 駒形修, 葛西真治, 川田均, 岡村佳香, 政田正弘, 冨田隆史
  • Organizer: 第55回日本応用動物昆虫学会大会
  • Place of Presentation: 大会中止により講演要旨集上発表
• [Presentation] 害虫の殺虫剤抵抗性機構
  • Author(s): 冨田隆史
  • Organizer: 第155回日本獣医学会学術集会／日本比較薬理・毒性学会 企画シンポジウム「薬剤耐性の分子メカニズム」
  • Place of Presentation: 東京大学駒場キャンパス，東京都
  • Invited
• [Remarks]
  • URL: http://www0.nih.go.jp/niid/entomology/index.html
• [Remarks] 国立感染症研究所昆虫医科学部
  • URL: http://www0.nih.go.jp/niid/entomology/
• [Remarks]
  • URL: http://www0.nih.go.jp/niid/entomology/papers/papers.html
• [Remarks]
  • URL: http://www.nih.go.jp/niid/ja/annual.html
• [Remarks]
  • URL: http://www.nih.go.jp/niid/entomology/index.html