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Characterization of organic nitrogen from cattle manure compost based on the molecular weight distribution

Research Project

Project/Area Number 22580073
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

MORIIZUMI Mihoko  独立行政法人農業・食品産業技術総合研究機構, 中央農業総合研究センター土壌肥料研究領域, 主任研究員 (10220039)

Co-Investigator(Kenkyū-buntansha) MATSUNAGA Toshiro  独立行政法人農業・食品産業技術総合研究機構, 中央農業総合研究センター土壌肥料研究領域, 上席研究員 (20355647)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsサイズ排除高速液体クロマトグラフィー / 化学発光窒素検出 / 土壌有機物 / 有機態窒素 / 堆肥 / 分子量分布 / アミノ酸 / サイズ排除クロマトグラフィー化学発光窒素検出法
Research Abstract

Hot water extractable organic nitrogen from cattle manure compost and from soil applied with the compost were separated by size-exclusion HPLC and detected by chemiluminescent nitrogen detection (CLND) and spectroscopic approaches. It is revealed that the organic nitrogen is composed of three different categories; (1) biomolecule (>100kDa), (2) humic acid like material (100-20kDa), and fulvic acid like material (<20kDa).

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (23 results)

All 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 3 results) Presentation (10 results) Book (3 results) Remarks (2 results)

  • [Journal Article] 土壌の可給態窒素分析法と課題2012

    • Author(s)
      松永俊朗・森泉美穂子
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 83 Pages: 625-629

    • NAID

      110009593773

    • URL

      http://jssspn.jp/book/magazine.html

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] 露地畑土壌における可給態窒素の各種簡易測定法の推定精度と抽出有機物の特性比較2012

    • Author(s)
      上薗一郎・森泉美穂子・加藤直人
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 83 Pages: 555-563

    • NAID

      110009593763

    • URL

      http://jssspn.jp/book/magazine.html

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] Characterization of protein-like fluorophores released from lake phytoplankton on the basis offractionation and electrophoresis2012

    • Author(s)
      E. Yamada, T. Hirota, N. Hatori, Y.Kitao, Y. Fuse, S. Aoki, H. Karatani and T. Matsunaga
    • Journal Title

      Anal. Sci

      Volume: 28 Pages: 595-600

    • Related Report
      2012 Final Research Report
  • [Journal Article] 地力窒素の分子実体は何か-土壌には多様な分子量の有機態窒素が存在する-2012

    • Author(s)
      森泉美穂子, 松永俊朗
    • Journal Title

      化学と生物

      Volume: 50 Pages: 239-241

    • NAID

      10030819378

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Characterization of protein-like fluorophores released from lake phytoplankton on the basis of fractionation and electrophoresis2012

    • Author(s)
      E. Yamada, T. Hirota, N. Hatori, Y. Kitao, Y. Fuse, S. Aoki, H.Karatani, T. Matsunaga
    • Journal Title

      Anal. Sci.

      Volume: 28 Pages: 595-600

    • NAID

      10030758052

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular weight separation ofhot-water extractable soil organic matter using high-performance size exclusion chromatography with chemiluminescent nitrogen detection. Soil Sci2011

    • Author(s)
      M.Moriizumi and T. Matsunaga
    • Journal Title

      Plant Nutr

      Volume: 57 Issue: 2 Pages: 185-189

    • DOI

      10.1080/00380768.2011.565478

    • Related Report
      2012 Final Research Report
  • [Journal Article] Molecular weight separation of hot-water extractable soil organic matter using high-performance size exclusion chromatography with chemiluminescent nitrogen detection2011

    • Author(s)
      M. Moriizumi, T. Matsunaga
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 57 Pages: 185-189

    • NAID

      110008897276

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular weight separation of hot-water extractable soil organic matter using high-performance size exclusion chromatography with chemiluminescent nitrogen detection2011

    • Author(s)
      Mihoko Moriizumi, Toshiro Matunaga
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: (印刷中)

    • NAID

      110008897276

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 水田土壌と畑土壌の80℃16時間水抽出有機態窒素の分子量分布の比較2012

    • Author(s)
      森泉美穂子・松永俊朗・加藤直人・東英男
    • Organizer
      2012年度日本土壌肥料学会関東支部大会
    • Place of Presentation
      つくば国際会議場,つくば市
    • Year and Date
      2012-12-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 堆肥添加土壌の長期培養における80℃16時間水抽出有機物の変化2012

    • Author(s)
      森泉美穂子・加藤直人・松永俊朗・東英男
    • Organizer
      2012年日本土壌肥料学会
    • Place of Presentation
      鳥取大学,鳥取市
    • Related Report
      2012 Final Research Report
  • [Presentation] Temporal change in molecular weight distribution of hot-water extractable organic nitrogen from cattle manure compost buried in soil using high-performance size exclusion chromatography with chemiluminescent nitrogen detection2012

    • Author(s)
      M. Moriizumi and T. Matsunaga
    • Organizer
      European Geosciencies Union 2012
    • Place of Presentation
      ウィーン国際会議場 ウィーン(オーストリア)
    • Related Report
      2012 Final Research Report
  • [Presentation] Temporal change in molecular weight distribution of hot-water extractable organic nitrogen from cattle manure compost buried in soil using high-performance size exclusion chromatography with chemiluminescent nitrogen detection2012

    • Author(s)
      M. Moriizumi, T.Mastunaga
    • Organizer
      European Geosciences Union 2012
    • Place of Presentation
      ウィーン国際会議場
    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
  • [Presentation] 水田土壌と畑土壌の80℃16 時間水抽出有機態窒素の分子量布の比較2012

    • Author(s)
      森泉美穂子, 松永俊朗,加藤直人,東英男
    • Organizer
      2012年度日本土壌肥料学会関東支部大会
    • Place of Presentation
      つくば市文部科学省 研究交流センター
    • Related Report
      2012 Annual Research Report
  • [Presentation] 堆肥添加土壌の長期培養における80℃16時間水抽出有機物の変化2012

    • Author(s)
      森泉美穂子,加藤直人,松永俊朗,東英男
    • Organizer
      2012年度日本土壌肥料学会
    • Place of Presentation
      鳥取大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 堆肥から熱水抽出された有機態窒素のサイズ排除HPLC/化学発光窒素検出による分離分析2011

    • Author(s)
      松永俊朗・森泉美穂子
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋大学,名古屋市
    • Related Report
      2012 Final Research Report
  • [Presentation] 堆肥から熱水抽出された有機態窒素のサイズ排除HPLC/化学発光窒素検出による分離分析2011

    • Author(s)
      松永俊朗, 森泉美穂子
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] サイズ排除クロマトグラフィー/化学発光窒素検出器(SEC-HPLC/CLND)による土壌有機物の分子量別窒素含有量の測定2010

    • Author(s)
      森泉美穂子・松永俊朗
    • Organizer
      日本腐植物質学会
    • Place of Presentation
      筑波大学
    • Year and Date
      2010-11-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] サイズ排除クロマトグラフィー/化学発光窒素検出器(SEC-HPLC/CLND)による土壌有機物の分子量別窒素含有量の測定2010

    • Author(s)
      森泉美穂子・松永俊朗
    • Organizer
      日本腐植物質学会
    • Place of Presentation
      筑波大学,つくば市
    • Year and Date
      2010-11-26
    • Related Report
      2012 Final Research Report
  • [Book] 地力窒素の分子実体は何か-土壌には多様な分子量の有機態窒素が存在する-2012

    • Author(s)
      森泉美穂子・松永俊朗
    • Publisher
      化学と生物
    • Related Report
      2012 Final Research Report
  • [Book] 土壌有機態窒素の分子化学的解明に向けて.田畑輪換土壌の肥沃度と管理2010

    • Author(s)
      森泉美穂子
    • Publisher
      博友社
    • Related Report
      2012 Final Research Report
  • [Book] 田畑輪換土壌の肥沃度と管理-変化の要因と制御の考え方3章 土壌有機態窒素の分子化学的解明に向けて2010

    • Author(s)
      森泉美穂子(日本土壌肥料学会編)
    • Publisher
      博友社
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://cse.naro.affrc.go.jp/tmatunag/soil_org_nitrogen/clnd.htm

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://cse.naro.affrc.go.jp/tmatunag/soil_org_nitrogen/clnd.htm

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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