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Community analysis of nodule-assoicated rhizosphere for revealing a mechanism of continuous cropping promlem in legumious plants

Research Project

Project/Area Number 22580074
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

IKEDA Seishi  独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター大規模畑作研究領域, 主任研究員 (20396609)

Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords根粒 / 連作障害 / マメ科 / 土壌微生物 / 根圏 / 細菌 / 糸状菌 / 線虫 / 真核微生物 / ダイズ / インゲン / 微生物多様性 / RISA / 連作 / 土壌DNA / 微生物 / 多様性 / 連鎖障害 / 生態学 / 土壌学 / 遺伝子 / 植物
Outline of Final Research Achievements

As a hypothesis, the nodules biomass in a field was considered as a main factor for causing problems in continuous cropping of leguminous plants. After continuous cropping of three different nodulation phenotypes of soybeans (non-nodule, super-nodule, and wild type soybeans) on the same fields for three years, kidney beans were cultivated on those field as a succeeding crop. The community analysis of rhizosphere soils of these kidney beans revealed that the populations of nematode had no correlation to the nodule biomass in a preceding crop. However, the community structure of non-nodulated soybean mutant was shown to be similar to that of kidney beans which had been continuously cropping for four years. In addition, the community analysis clarified that the relative abundance of Mortierella elongate was increased in the rhizosphere of kidney beans as responding to the nodule biomass of preceding crops.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (47 results)

All 2015 2014 2013 2012 2011 2010

All Journal Article (23 results) (of which Peer Reviewed: 19 results,  Open Access: 8 results,  Acknowledgement Compliant: 8 results) Presentation (22 results) (of which Invited: 2 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Bacterial community shifts associated with high abundance of Rhizobium spp. in potato roots under macronutrient-deficient conditions2015

    • Author(s)
      Yusuke Unno, Takuro Shinano, Kiwamu Minamisawa, Seishi Ikeda
    • Journal Title

      Soil Biology & Biochemistry

      Volume: 80 Pages: 232-236

    • DOI

      10.1016/j.soilbio.2014.10.002

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Elevated atmospheric CO2 levels affect community structure of rice root-associated bacteria2015

    • Author(s)
      Takashi Okubo, Dongyan Liu, Hirohito Tsurumaru, Seishi Ikeda, Susumu Asakawa, Takeshi Tokida, Kanako Tago, Masahito Hayatsu, Naohiro Aoki, Ken Ishimaru, Kazuhiro Ujiie, Yasuhiro Usui, Hirofumi Nakamura, Hidemitsu Sakai, Kentaro Hayashi, Toshihiro Hasegawa and Kiwamu Minamisawa
    • Journal Title

      Frontiers in Microbiology

      Volume: 6 Pages: 1-8

    • DOI

      10.3389/fmicb.2015.00136

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Metagenomic Analysis of the Bacterial Community Associated with the Taproot of Sugar Beet2015

    • Author(s)
      Hirohito Tsurumaru, Takashi Okubo, Kazuyuki Okazaki, Megumi Hashimoto, Kaori Kakizaki, Eiko Hanzawa, Hiroyuki Takahashi, Noriyuki Asanome, Fukuyo Tanaka, Yasuyo Sekiyama, Seishi Ikeda, and Kimwamu Minamisawa
    • Journal Title

      Microbes and Environments

      Volume: 30 Issue: 1 Pages: 63-69

    • DOI

      10.1264/jsme2.ME14109

    • NAID

      130005061718

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of Leaf Blade- and Leaf Sheath-Associated Bacterial Communities and Assessment of Their Responses to Environmental Changes in CO<sub>2</sub>, Temperature, and Nitrogen Levels under Field Conditions2015

    • Author(s)
      Seishi Ikeda, Takeshi Tokida, Hirofumi Nakamura, Hidemitsu Sakai, Yasuhiro Usui, Takashi Okubo, Kanako Tago, Kentaro Hayashi, Yasuyo Sekiyama, Hiroshi Ono, Satoru Tomita, Masahito Hayatsu, Toshihiro Hasegawa, and Kiwamu Minamisawa
    • Journal Title

      Microbes and Environments

      Volume: 30 Issue: 1 Pages: 51-62

    • DOI

      10.1264/jsme2.ME14117

    • NAID

      130005061713

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Low Nitrogen Fertilization Adapts Rice Root Microbiome to Low Nutrient Environment by Changing Biogeochemical Functions2014

    • Author(s)
      Seishi Ikeda, Kazuhiro Sasaki, Takashi Okubo, Akifumu Yamashita, Kimihiro Terasawa, Zhihua Bao, Dongyan Liu, Takeshi Watanabe, Jun Murase, Susumu Asakawa, Shima Eda, Hisayuki Mitsui, Tadashi Sato, and Kiwamu Minamisawa
    • Journal Title

      Microbes and Environments

      Volume: 29 Issue: 1 Pages: 50-59

    • DOI

      10.1264/jsme2.ME13110

    • NAID

      130003390907

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] An Assessment of the Diversity of Culturable Bacteria from Main Root of Sugar Beet2014

    • Author(s)
      Kazuyuki Okazaki, Takao Iino, Yosuke Kuroda, Kazunori Taguchi, Hiroyuki Takahashi, Takuji Ohwada, Hiroto Tsurumaru, Takashi Okubo, Kiwamu Minamisawa, and Seishi Ikeda
    • Journal Title

      Microbes and Environments

      Volume: 29 Issue: 2 Pages: 220-223

    • DOI

      10.1264/jsme2.ME13182

    • NAID

      130004057181

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Phylogeny and Functions of Bacterial Communities Associated with Field-Grown Rice Shoots2014

    • Author(s)
      Takashi Okubo, Seishi Ikeda, Kazuhiro Sasaki, Kenshiro Ohshima, Masahira Hattori, Tadashi Sato, and
    • Journal Title

      Microbes and Environments

      Volume: 29 Issue: 3 Pages: 329-332

    • DOI

      10.1264/jsme2.ME14077

    • NAID

      130004677526

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] An Assessment of Urea-Formaldehyde Fertilizer on the Diversity of Bacterial Communities in Onion and Sugar Beet2014

    • Author(s)
      Seishi Ikeda, Keijiro Suzuki, Makoto Kawahara, Masao Noshiro, and Naokazu Takahashi
    • Journal Title

      Microbes and Environments

      Volume: 29 Issue: 2 Pages: 231-234

    • DOI

      10.1264/jsme2.ME13157

    • NAID

      130004057177

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 植物共生科学の新展開と農学研究におけるパラダイムシフト2013

    • Author(s)
      池田成志、鶴丸博人、大久保卓、岡崎和之、南澤究、
    • Journal Title

      化学と生物

      Volume: 7 Pages: 462-470

    • NAID

      10031184722

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Characterization of the Bacterial Community in a Potato Phytosphere2013

    • Author(s)
      Someya, N., Y.O. Kobayashi, S. Tsuda and S. Ikeda
    • Journal Title

      Microbes and Environments

      Volume: 28 Issue: 3 Pages: 295-305

    • DOI

      10.1264/jsme2.ME13006

    • NAID

      10031191412

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 植物生育促進細菌の研究動向2013

    • Author(s)
      鶴丸博人、橋本萌、池田成志、南澤究
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 84 Pages: 418-423

    • NAID

      110009766666

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 環境微生物研究とリスク管理2013

    • Author(s)
      池田成志
    • Journal Title

      日本微生物生態学会誌

      Volume: 28 Pages: 75-76

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 植物共生微生物と「土づくり」2013

    • Author(s)
      池田 成志
    • Journal Title

      あぐりぽーと

      Volume: 101 Pages: 6-6

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 生産現場で働く微生物資材・連載3・植物共生微生物と農業・その22012

    • Author(s)
      池田 成志
    • Journal Title

      ニューカントリー

      Volume: 7 Pages: 50-52

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 生産現場で働く微生物資材・連載3・植物共生微生物と農業・その12012

    • Author(s)
      池田 成志
    • Journal Title

      ニューカントリー

      Volume: 6 Pages: 52-53

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Pyrosequence Read Length of 16S rRNA Gene Affects Phylogenetic Assignment of Plant-associated Bacteria2012

    • Author(s)
      大久保 卓
    • Journal Title

      Microbes and Environments

      Volume: 27 Issue: 2 Pages: 204-208

    • DOI

      10.1264/jsme2.ME11258

    • NAID

      10030432208

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitrogen Cycling in Rice Paddy Environments: Past Achievements and Future Challenges2011

    • Author(s)
      Ishii, S.
    • Journal Title

      Microbes and Environments

      Volume: 26 Issue: 4 Pages: 282-292

    • DOI

      10.1264/jsme2.ME11293

    • NAID

      10029977814

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Genotype of the Calcium/Calmodulin-Dependent Protein Kinase Gene (CCaMK) Determines Bacterial Community Diversity in Rice Roots under Paddy and Upland Field Conditions2011

    • Author(s)
      Ikeda, S.
    • Journal Title

      Applied Environmental Microbiology

      Volume: 77 Issue: 13 Pages: 4399-4405

    • DOI

      10.1128/aem.00315-11

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and Genetic Characterization of Aurantimonas and Methylobacterium Strains from Stems of Hypernodulated Soybeans2011

    • Author(s)
      Anda, M.
    • Journal Title

      Microbes and Environments

      Volume: 26 Issue: 2 Pages: 172-180

    • DOI

      10.1264/jsme2.ME10203

    • NAID

      10029123041

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autoregulation of nodulation interferes with impacts of nitrogen fertilization levels on the leaf-associated bacterial community in soybeans2011

    • Author(s)
      Ikeda, S.
    • Journal Title

      Applied Environmental Microbiology

      Volume: 77 Issue: 6 Pages: 1973-1980

    • DOI

      10.1128/aem.02567-10

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A rapid and simple PCR method for identifying isolates of the genus Azospirillum within populations of rhizosphere bacteria2011

    • Author(s)
      Shime, A.
    • Journal Title

      Journal of Applied Microbiology

      Volume: 111 Pages: 915-924

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autoregulation of Nodulation Interferes with Impacts of Nitrogen Fertilization Levels on the Leaf-Associated Bacterial Community in Soybeans2011

    • Author(s)
      Ikeda, et al.
    • Journal Title

      Ikeda, S., M.Anda, S.Inaba, S.Eda, S.Sato, K.Sasaki, S.Tabata, H.Mitsui, T.Sato, T.Shinano, K.Minamisawa

      Volume: 77 Pages: 1973-1980

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Community- and Genome-Based Views of Plant-Associated Bacteria : Plant-Bacterial Interactions in Soybean and Rice2010

    • Author(s)
      Ikeda, S., T.Okubo, M.Anda, H.Nakashita, M.Yasuda, S.Sato, T.Kaneko, S.Tabata, S.Eda, A.Momiyama, K.Terasawa, H.Mitsui, K.Minamisawa
    • Journal Title

      Plant and Cell Physiology

      Volume: 51 Pages: 1398-1410

    • NAID

      10027351961

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 根粒根圏土壌微生物相の群集構造解析によるマメ科作物の連作障害の発生機構の解明2014

    • Author(s)
      池田成志、臼木一英、南澤 究
    • Organizer
      日本土壌肥料学会北海道支部会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-12-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] 植物共生科学の新展開と農学研究のパラダイムシフト2014

    • Author(s)
      池田成志、南澤究
    • Organizer
      日本生物工学会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-09-09
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Molecular community analysis for unraveling plant-microbes interactions in arable land2014

    • Author(s)
      Seishi Ikeda and Kiwamu Minamisawa
    • Organizer
      the 6th East Asian Federation of Ecological Societies International Congress
    • Place of Presentation
      Haikou, China
    • Year and Date
      2014-04-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ジャガイモそうか病に対する抵抗性レベルの異なるジャガイモ8品種間における根および塊茎関連細菌群集の構造比較2012

    • Author(s)
      小林晃
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-03-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物共生微生物と農業2012

    • Author(s)
      池田成志
    • Organizer
      農の周辺講座、有機実業、大牧牧場、共催
    • Place of Presentation
      とかちプラザ(招待講演)
    • Year and Date
      2012-03-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物共生科学の新展開と農業再考2012

    • Author(s)
      池田成志
    • Organizer
      茨城大学推進研究プロジェクト公開シンポジウム
    • Place of Presentation
      茨城大学(招待講演)
    • Year and Date
      2012-02-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物共生科学の新展開と農業への応用について2012

    • Author(s)
      池田 成志
    • Organizer
      平成24年度日本微生物生態学会大会
    • Place of Presentation
      豊橋科学技術大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 植物共生微生物相の解析による有機栽培作物の特性評価の試み2011

    • Author(s)
      池田成志
    • Organizer
      日本有機農業学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-12-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 緩効性肥料が植物共生微生物相に及ぼす影響2011

    • Author(s)
      池田成志
    • Organizer
      日本土壌肥料学会北海道支部秋季大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-12-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物共生微生物と農業2011

    • Author(s)
      池田成志
    • Organizer
      北農会第2回シンポジウム
    • Place of Presentation
      北農健保会館(招待講演)
    • Year and Date
      2011-11-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] ジャガイモから分離した蛍光性シュードモナス菌株の多様性2011

    • Author(s)
      染谷信孝
    • Organizer
      日本土壌微生物学会
    • Place of Presentation
      鳴子公民館
    • Year and Date
      2011-11-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 有機栽培ジャガイモの根系に共生する細菌群集の構造解析2011

    • Author(s)
      池田成志
    • Organizer
      日本土壌微生物学会大会
    • Place of Presentation
      鳴子公民館
    • Year and Date
      2011-11-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] A rapid and simple PCR method to identify isolates belonging to the Genus Azospirillum2011

    • Author(s)
      小林創平
    • Organizer
      10th international conference of the east and southeast Asiafederation of soil science societies
    • Place of Presentation
      Cinnamon Lakeside, Colombo, Sri Lanka
    • Year and Date
      2011-10-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 非培養法および培養によるジャガイモ共生細菌の多様性比較2011

    • Author(s)
      染谷信孝
    • Organizer
      日本微生物生態学会大会
    • Place of Presentation
      京都大学
    • Year and Date
      2011-10-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Taxonomic and ecological evaluation of actinomycetes isolated in Vietnam2011

    • Author(s)
      崎山弥生
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] A rapid and simple PCR-based detection method targeting the genus Azospirillum2011

    • Author(s)
      Shime, A.
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Comparison of root- and tuber-associated bacterial communities between common scab-resistant and -susceptible barieties of potato2011

    • Author(s)
      小林有紀
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Genetic diversity of fluorescent pseudomonads in the phyllosphere and rhizosphere of potato plants in Japan2011

    • Author(s)
      染谷信孝
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular diversity of potato-associated bacteria2011

    • Author(s)
      染谷信孝
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物共生科学の新展開と肥培管理の再考2011

    • Author(s)
      池田成志
    • Organizer
      平成23年度第60回北海道高等学校農業教育研究大会/第15回全国高等学校農業協会北海道支部大会・分科会
    • Place of Presentation
      とかちプラザ
    • Year and Date
      2011-07-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物共生科学の新展開と肥培管理の再考2011

    • Author(s)
      池田成志
    • Organizer
      NPO法人有機農業技術会議・第4回有機農業技術原論研究会
    • Place of Presentation
      アジア太平洋資料センター(招待講演)
    • Year and Date
      2011-06-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] OsCCaMKは水稲根と陸稲根の細菌群集構造を変化させる2011

    • Author(s)
      南澤究
    • Organizer
      植物微生物研究会第21回研究交流会
    • Place of Presentation
      岡山大学創立50周年記念館(岡山)
    • Related Report
      2011 Annual Research Report
  • [Book] 改訂新編土壌微生物実験法(13-4 RISA)2013

    • Author(s)
      池田成志(分担)
    • Publisher
      養賢堂
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 植物の生育促進剤2013

    • Inventor(s)
      岡崎和之、池田成志、他8名
    • Industrial Property Rights Holder
      農業・食品産業技術総合研究機構
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-146402
    • Filing Date
      2013-07-12
    • Related Report
      2013 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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