Regulation mechanism of intracellular coenzyme A by pantothenate kinase in microbes

• Project/Area Number: 22580075
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Ibaraki University
• Principal Investigator: CHOHNAN Shigeru 茨城大学, 農学部, 准教授 (70312775)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: パントテン酸キナーゼ / コエンザイムA / 真正細菌 / 古細菌 / 代謝調節 / 微生物 / 補酵素 / ビタミン / パント酸キナーゼ

Research Abstract

Coenzyme A (CoA) functions as a carbon carrier. To clarify the regulation mechanism of the biosynthetic pathway of this important coenzyme, pantothenate kinase (CoaA) being the key enzyme was analyzed, and the following points were clarified. i) The enzymes which function in archaeal CoA biosynthetic pathway are not CoaAs which is commonly used in bacteria and eukaryotes but the intrinsic ones to archaea. ii) Two CoaA homologues in Bacillus subtilis have the enzyme activity, suggesting the possibility that both CoaAs are active in vivo.

Research Products

• [Journal Article] Role of prokaryotic type I and III pantothenate kinases in the coenzyme A biosynthetic pathway of Bacillus subcilis (2014)
  • Author(s): Yuta Ogata, Hiroki Katoh, Munehiko Asayama, Shigeru Chohnan
  • Journal Title: Canadian Journal of Microbiology Volume: 60 Issue: 5 Pages: 297-305
  • DOI: 10.1139/cjm-2013-0793
  • Peer Reviewed
• [Journal Article] Identification of pantoate kinase and phosphopantoate synthetase from Methanospirillum hungatei (2013)
  • Author(s): Hiroki Katoh, Hideyuki Tanaki, Yuka Tokutake, Satoshi Hanada, Shigeru Chohnan
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 115巻 Issue: 4 Pages: 372-376
  • DOI: 10.1016/j.jbiosc.2012.10.019
  • Peer Reviewed
• [Presentation] 枯草菌のコエンザイムA生合成 (2013)
  • Author(s): 緒形雄太、朝山宗彦、長南茂
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学
  • Year and Date: 2013-03-26
• [Presentation] 枯草菌のコエンザイムA生合成 (2013)
  • Author(s): 緒形雄太、朝山宗彦、長南茂
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東北大学（宮城県）
• [Presentation] Characterization of prokaryotic types I and III pantothenate kinases from Bacillus subtilis (2012)
  • Author(s): Yuta Ogata, Yuka Tokutake, Shigeru Chohnan
  • Organizer: 112th General Meeting, American Society for Microbiology
  • Place of Presentation: Moscone Convention Center at San Francisco(アメリカ)
  • Year and Date: 2012-06-19
• [Presentation] Characterization of prokaryotic types I and III pantothenate kinases from Bacillus subtilis (2012)
  • Author(s): Yuta Ogata, Tuka Tokutake, Shigeru Chohnan
  • Organizer: American Society for Microbiology
  • Place of Presentation: Moscone Convention Center (San Francisco, USA)
• [Presentation] 枯草菌の原核I型およびIII型パントテン酸キナーゼの性質 (2011)
  • Author(s): 緒形雄太、長南茂
  • Organizer: 第27回日本微生物生態学会大会
  • Place of Presentation: 京都大学
  • Year and Date: 2011-10-09
• [Presentation] 枯草菌の原核I型およびIII型パントテン酸キナーゼの性質 (2011)
  • Author(s): 緒形雄太、長南茂
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2011-10-09
• [Presentation] A coenzyme A biosynthetic pathway in archaea (2011)
  • Author(s): Hiroki Katoh, Hideyuki Tamaki, Yuka Tokutake, Satoshi Hanada, Shigeru Chohnan
  • Organizer: 111th General Meeting, American Society for Microbiology
  • Place of Presentation: Ernest N. Morial Convention Center at New Orleans(アメリカ)
  • Year and Date: 2011-05-24
• [Presentation] A coenzyme A biosynthetic pathway in archaea (2011)
  • Author(s): 加藤広己、玉木秀幸、徳竹由華、花田智、長南茂
  • Organizer: アメリカ微生物学会
  • Place of Presentation: アメリカ合衆国・ニューオリンズ
  • Year and Date: 2011-05-24
• [Presentation] メタン生成古細菌のコエンザイムA生合成経路 (2010)
  • Author(s): 加藤広己、長南茂
  • Organizer: 日本微生物生態学会第26回大会
  • Place of Presentation: 筑波大学
  • Year and Date: 2010-11-25
• [Presentation] Pantothenate kinase from thermoacidophilic archaeon (2010)
  • Author(s): Masakazu Takagi, Yukiko Miyamoto, Hedeyuki Tamaki, Roberta Leonardi, Satoshi Hanada, Suzanne Jackowski, Shigeru Chohnan
  • Organizer: 110th General Meeting, American Society for Microbiology
  • Place of Presentation: San Diego Convention Center(アメリカ)
  • Year and Date: 2010-05-24
• [Presentation] メタン生成古細菌のコエンザイムA生合成経路 (2010)
  • Author(s): 加藤広己、長南茂
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 筑波大学(つくば市)
• [Remarks]
  • URL: http://info.ibaraki.ac.jp/Profiles/5/0000444/profile.html
• [Remarks] 茨城大学研究者情報総覧
  • URL: http://info.ibaraki.ac.jp/Profiles/5/0000444/profile.html
• [Remarks] 茨城大学研究者情報総覧
  • URL: http://info.ibaraki.ac.jp/Profiles/5/0000444/profile.html
• [Remarks] 茨城大学研究者情報総覧
  • URL: http://info.ibaraki.ac.jp/scripts/websearch/index.htm