Gene expression mechanism by LysR-type transcriptional regulator and basis for self-recognition
| Project/Area Number |
22580078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Shizuoka University |
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Principal Investigator |
OGAWA Naoto 静岡大学, 農学部, 教授 (60354031)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 遺伝子発現 / LysRタイプ転写調節因子 / 転写 / プロモーター / 遺伝子 / 発現制御 / 応用微生物 / 細菌 / 分子生物学 |
|---|
| Research Abstract |
The LysR-type transcriptional regulators (LTTRs) comprise one of the largest families of bacterial transcriptional regulators, involved in expression of many genes of diverse roles such as amino acid biosynthesis, degradation of aromatic compounds, and virulence factor synthesis. This study revealed important amino acids for DNA binding and inducer recognition by LTTR in relation to three dimensional structure, and established methods for purifying protein and DNA for co-crystallization of LTTR and the regulated promoter, which together will lead to elucidation of mechanism of transcriptional activation by LTTRs.
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Report
(4 results)
Research Products
(13 results)
