Gene expression mechanism by LysR-type transcriptional regulator and basis for self-recognition

• Project/Area Number: 22580078
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Shizuoka University
• Principal Investigator: OGAWA Naoto 静岡大学, 農学部, 教授 (60354031)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 遺伝子発現 / LysRタイプ転写調節因子 / 転写 / プロモーター / 遺伝子 / 発現制御 / 応用微生物 / 細菌 / 分子生物学

Research Abstract

The LysR-type transcriptional regulators (LTTRs) comprise one of the largest families of bacterial transcriptional regulators, involved in expression of many genes of diverse roles such as amino acid biosynthesis, degradation of aromatic compounds, and virulence factor synthesis. This study revealed important amino acids for DNA binding and inducer recognition by LTTR in relation to three dimensional structure, and established methods for purifying protein and DNA for co-crystallization of LTTR and the regulated promoter, which together will lead to elucidation of mechanism of transcriptional activation by LTTRs.

Research Products

• [Journal Article] Specific gene responses of a gram-positive polychlorinated- biphenyl degrader (2012)
  • Author(s): Toju Iino, Yong Wang, Keisuke Miyauchi, Daisuke Kasai, Eiji Masai, Takeshi Fujii, Naoto Ogawa, and Masao Fukuda
  • Journal Title: Rhodococcus jostiiRHA1 during growth in soil. Appl. Environ. Microbiol Volume: Vol.78 Pages: 6954-6962
  • Peer Reviewed
• [Journal Article] A survey of the cellular responses in Pseudomonas putida KT2440 growing in sterilized soil by microarray analysis (2011)
  • Author(s): Yong Wang, Sho Morimoto, Naoto Ogawa, and Takeshi Fujii
  • Journal Title: FEMS Microbiol. Ecol. Volume: Vol.78 Pages: 220-232
  • Peer Reviewed
• [Journal Article] Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways (2011)
  • Author(s): Tsuneo Ohmori, Hirokazu Morita, Megumi Tanaka, Keisuke Miyauchi, Daisuke Kasai, Kensuke Furukawa, Kiyotaka Miyashita, Naoto Ogawa, Eiji Masai, and Masao Fukuda
  • Journal Title: J. Biosci. Bioeng Volume: Vol.111 Pages: 437-442
  • NAID: 110008607499
  • Peer Reviewed
• [Journal Article] 日本土壌肥料学雑誌進歩総説「土壌生物」 (2011)
  • Author(s): 佐伯和利、村上弘治、佐伯雄一、坂本一憲、村瀬潤、鮫島玲子、小川直人、浅川晋
  • Journal Title: 日本土壌肥料学雑誌 Volume: 第82巻 Pages: 479-494
• [Journal Article] Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways (2011)
  • Author(s): Tsuneo Ohmori, Naoto Ogawa, 他
  • Journal Title: Journal of Bioscience and Bioengineering Volume: Vol.111 Pages: 437-442
  • NAID: 110008607499
  • Peer Reviewed
• [Journal Article] A survey of the cellular responses in Pseudomonas putida KT2440 growing in sterilized soil by microarray analysis (2011)
  • Author(s): Yong Wang, Naoto Ogawa, 他
  • Journal Title: FEMS Microbiology and Ecology Volume: Vol.78 Pages: 220-232
  • Peer Reviewed
• [Presentation] Analysis of CbnR, a bacterial LysR-type transcriptional regulator, indicates a conserved aspect of DNA binding and distinctive schemes of inducer recognition (2012)
  • Author(s): Naoto Ogawa, Kaori Takada, Masae Takabayashi.
  • Organizer: 日本微生物生態学会第28回大会
  • Place of Presentation: 豊橋技術科学大学
• [Presentation] Analysis of CbnR, a bacterial LysR-type transcriptional regulator, indicates a conserved aspect of DNA binding and distinctive schemes of inducer recognition (2012)
  • Author(s): Naoto Ogawa
  • Organizer: 日本微生物生態学会第28回大会
  • Place of Presentation: 豊橋技術科学大学（愛知県）
• [Presentation] Transcriptomic analysis revealed a highly responsive gene cluster to 3-c hlorobenzoic acid in Pseudomonas putida KT2440 growing in sterilized soil (2011)
  • Author(s): Yong Wang, Naoto Ogawa, 他
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-14
• [Presentation] Cupriavidus necatorNH9 株の LysR タイプ転写調節因子CbnR のDNA 結合領域の解析 (2011)
  • Author(s): 高田香織、下平潤、黒田直子、秋山絵弥子、千田俊哉、小川直人.
  • Organizer: 日本農芸化学会
  • Place of Presentation: 震災の影響で要旨集の発刊をもって開催に代えた
  • Year and Date: 2011-03-27
• [Presentation] Cupriavidus necatorNH9 株の LysR タイプ発現調節因子 CbnR の誘導物質認識部位の解析 (2011)
  • Author(s): 高林正恵、郎剛華、小川直人
  • Organizer: 日本農芸化学会
  • Place of Presentation: 震災の影響で要旨集の発刊をもって開催に代えた
  • Year and Date: 2011-03-27
• [Presentation] Cupriavidus necator NH9株のLysRタイプ発現調節因子CbnRの誘導物質認識部位の解析 (2011)
  • Author(s): 高林正恵、小川直人, 他
  • Organizer: 日本農芸化学会
  • Place of Presentation: (震災の影響で要旨集の発刊をもって開催に代えた)
  • Year and Date: 2011-03-27
• [Presentation] Cupriavidus necator NH9株のLysRタイプ発現調節因子CbnRのDNA結合領域の解析 (2011)
  • Author(s): 高田香織、小川直人, 他
  • Organizer: 日本農芸化学会
  • Place of Presentation: (震災の影響で要旨集の発刊をもって開催に代えた)
  • Year and Date: 2011-03-27