Expressional and functional characterization of the novel transporter protein related to thiocyanate ion uptake

• Project/Area Number: 22580082
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kyoto University
• Principal Investigator: ARAKAWA Takatoshi 京都大学, 医学研究科, 研究員 (30523766)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 微生物代謝 / ABCトランスポーター / 膜タンパク質 / 輸送体タンパク / チオシアネート / 硫化カルボニル / リポソーム / 共発現 / 蛋白質異種発現系

Research Abstract

Thiocyanate hydrorase (SCNase) operon contains four unidentified genes at downstream of the ones encoding the respective subunits of SCNase. The current research aimed at substantial characterization of the gene products using bacterial and yeast recombinant expression system. To date, while putative nucleotide binding domainexpressed as a soluble protein at considerable amount, the other three products yielded at lower level.

Research Products

• [Journal Article] Two arginine residues in the substrate pocket predominantly control the substrate selectivity of thiocyanate hydrolase (2013)
  • Author(s): Yasuaki Yamanaka, Takatoshi Arakawa, Toshinori Watanabe, Satoshi Namima, Masa Sato, Shota Hori, Akashi Ohtaki, Keiichi Noguchi, Yoko Katayama, Masafumi Yohda, Masafumi Odaka
  • Journal Title: Journal of Bioscience and Bioengineering Volume: Vol. 116、Issue 1 Issue: 1 Pages: 22-27
  • DOI: 10.1016/j.jbiosc.2013.01.013
  • NAID: 110009634740
  • Peer Reviewed