Expressional and functional characterization of the novel transporter protein related to thiocyanate ion uptake

Research Project

Project/Area Number	22580082
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Applied microbiology
Research Institution	Kyoto University
Principal Investigator	ARAKAWA Takatoshi 京都大学, 医学研究科, 研究員 (30523766)
Project Period (FY)	2010 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	微生物代謝 / ABCトランスポーター / 膜タンパク質 / 輸送体タンパク / チオシアネート / 硫化カルボニル / リポソーム / 共発現 / 蛋白質異種発現系
Research Abstract	Thiocyanate hydrorase (SCNase) operon contains four unidentified genes at downstream of the ones encoding the respective subunits of SCNase. The current research aimed at substantial characterization of the gene products using bacterial and yeast recombinant expression system. To date, while putative nucleotide binding domainexpressed as a soluble protein at considerable amount, the other three products yielded at lower level.