| Project/Area Number |
22580088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kagawa University |
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Principal Investigator |
TAKATA Goro 香川大学, 希少糖研究センター, 准教授 (50322722)
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| Co-Investigator(Kenkyū-buntansha) |
MORIMOTO Kenji 香川大学, 希少糖研究センター, 准教授 (90363184)
YOSHIHARA Akihide 香川大学, 希少糖研究センター, 助教 (40548765)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 微生物酵素 / 糖質科学 / 糖リン酸 / D-アロース / オリゴ糖 / 希少糖 / ホスホリラーゼ |
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| Research Abstract |
This research objective prospected to produce rare disaccharide and oligosaccharides by biotechnological methods. First, the material, D-allose was produced from starch by ethanol method. Although we tried to produce D-allose 1-phosphate via D-allose 6-phosphate, the efficiency was quite low. Therefore, we tried to produce this from glucose 1-phosphate using glucoside 3-dehydrogenase newly isolated by ourselves. We also investigate the oxidation of cellobiose using this microbe. As a result, we succeeded to oxidize cellobiose to ketocellobiose. By following reduction of sodium borohydride, we also succeeded to produce objective alosyl-allose at higher efficiency.
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