Production of Rare Oligosaccharides Using Microbial Enzymes

• Project/Area Number: 22580088
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kagawa University
• Principal Investigator: TAKATA Goro 香川大学, 希少糖研究センター, 准教授 (50322722)
• Co-Investigator(Kenkyū-buntansha): MORIMOTO Kenji 香川大学, 希少糖研究センター, 准教授 (90363184) ; YOSHIHARA Akihide 香川大学, 希少糖研究センター, 助教 (40548765)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 微生物酵素 / 糖質科学 / 糖リン酸 / D-アロース / オリゴ糖 / 希少糖 / ホスホリラーゼ

Research Abstract

This research objective prospected to produce rare disaccharide and oligosaccharides by biotechnological methods. First, the material, D-allose was produced from starch by ethanol method. Although we tried to produce D-allose 1-phosphate via D-allose 6-phosphate, the efficiency was quite low. Therefore, we tried to produce this from glucose 1-phosphate using glucoside 3-dehydrogenase newly isolated by ourselves. We also investigate the oxidation of cellobiose using this microbe. As a result, we succeeded to oxidize cellobiose to ketocellobiose. By following reduction of sodium borohydride, we also succeeded to produce objective alosyl-allose at higher efficiency.

Research Products

• [Journal Article] Gene Cloning and Characterization of L-Ribulose 3-epimerase from Mesorhizobium loti and Its Application to Rare Sugar Production. (2013)
  • Author(s): Uechi, K., Takata, G., Fukai, Y., Yoshihara, A., Morimoto, K.
  • Journal Title: Biosci Biotechnol Biochem Volume: 77(3) Pages: 511-5
  • NAID: 10031164769
• [Journal Article] Preparation ofD-gulose from disaccharide lactitol using microbial and chemical methods (2013)
  • Author(s): Morimoto, K., Shimonishi, T., Miyake, S., Takata, G., Izumori, K.
  • Journal Title: BiosciBiotechnol Biochem Volume: 77(2) Pages: 253-8
• [Journal Article] Cloning and characterization of the L-ribose isomerase gene from Cellulomonas parahominis MB426. (2013)
  • Author(s): Morimoto, K., Terami, Y., Maeda, Y., Yoshihara, A., Takata, G., Izumori, K.
  • Journal Title: J Biosci Bioeng
  • NAID: 110009603683
• [Journal Article] Preparation of D-gulose from disaccharide lactitol using microbial and chemical methods. (2013)
  • Author(s): Morimoto, K., Shimonishi, T., Miyake, S., Takata, G., Izumori, K.
  • Journal Title: Biosci Biotechnol Biochem Volume: 77 Pages: 253-258
  • NAID: 10031164724
  • Peer Reviewed
• [Journal Article] Cloning and characterization of the l-ribose isomerase gene from Cellulomonas parahominis MB426. (2013)
  • Author(s): Morimoto, K., Terami, Y., Maeda, YI., Yoshihara, A., Takata, G., Izumori, K.
  • Journal Title: J Biosci Bioeng Volume: 115 Issue: 4 Pages: 377-381
  • DOI: 10.1016/j.jbiosc.2012.10.021
  • NAID: 110009603683
  • Peer Reviewed
• [Journal Article] Characterization of Mesorhizobium loti L-Rhamnose Isomerase and Its Application to L-Talose Production (2011)
  • Author(s): Takata, G., Uechi, K., Taniguchi, E., Kanbara, Y., Yoshihara, A., Morimoto, K., Izumori, K
  • Journal Title: Biosci Biotechnol Biochem Volume: 75(5) Pages: 1006-1009
  • NAID: 10028273062
• [Journal Article] Enzymatic and Mutational Analyses of a Class II 3,5-Cyclic Nucleotide Phosphodiesterase, PdeE, from Myxococcus xanthus (2011)
  • Author(s): Kimura, Y., Yoshimi, M., Takata, G.
  • Journal Title: J Bacteriol Volume: 193(8) Pages: 2053-2057
• [Journal Article] Enzymatic and Mutational Analyses of a Class II 3,5-Cyclic Nucleotide Phosphodiesterase, PdeE, from Myxococcus xanthus (2011)
  • Author(s): Kimura, Y., Yoshimi, M., Takata, G.
  • Journal Title: Journal of Bacteriology Volume: 193 Pages: 2053-2057
  • Peer Reviewed
• [Journal Article] Characterization of Mesorhizobium loti L-Rhamnose Isomerase and Its Application to L-Talose Production (2011)
  • Author(s): Takata, G., Uechi, K., Taniguchi, E., Kanbara, Y., Yoshihara, A., Morimoto, K., Izumori, K.
  • Journal Title: Bioscience, Biotechnology and Biochemistry Volume: 75 Pages: 1006-1009
  • NAID: 10028273062
  • Peer Reviewed
• [Journal Article] Characterization of Mesorhizobium loti L-Rhamnose Isomerase and Its Application to L-Talose Production. (2011)
  • Author(s): Takata, G., et al.
  • Journal Title: Bioscience, Biotechnology and Biochemistry Volume: (In press 掲載決定)
  • NAID: 10028273062
  • Peer Reviewed
• [Journal Article] Enzymatic and Mutational Analyses of a Class II 3,5-Cyclic Nucleotide Phosphodiesterase, PdeE, from Myxococcus xanthus. (2011)
  • Author(s): Kimura, Y., et al.
  • Journal Title: Journal of Bacteriology Volume: 193 Pages: 2053-2057
  • Peer Reviewed
• [Journal Article] Cloning and overexpression of the xylitol dehydrogenase gene from Bacillus pallidus and its application to L-Xylulose production (2010)
  • Author(s): Takata, G., Poonperm, W., Morimoto, K., Izumori, K.
  • Journal Title: Cloning and overexpression of the xylitol dehydrogenase gene from Bacillus pallidus and its application to L-Xylulose production Volume: 74 (9) Pages: 1807-1813
  • NAID: 10027559294
• [Journal Article] Cloning and overexpression of the xylitol dehydrogenase gene from Bacillus pallidus and its application to L-Xylulose production. (2010)
  • Author(s): Takata, G., et al.
  • Journal Title: Bioscience, Biotechnology and Biochemistry Volume: 74 Pages: 1807-1813
  • NAID: 10027559294
  • Peer Reviewed
• [Presentation] Raoultella ornithinolytica MB426株由来のL-リボースイソメラーゼを用いたL-アロースの生産 (2012)
  • Author(s): 寺見優司、吉原明秀、森本兼司、高田悟郎
  • Organizer: 日本農芸化学会2012年度全国大会
  • Place of Presentation: 京都府
  • Year and Date: 2012-03-23
• [Presentation] Characterization of two ketose 3-epimerase from Mesorhizobium loti tono and its application of rare sugar production (2012)
  • Author(s): Uechi, K., Yoshihara, A., Morimoto, K., Takata, G.
  • Organizer: Bioactive Okayama 2012
  • Place of Presentation: 岡山大学
• [Presentation] Efficient bioproduction of rare sugars using microbial and chemical reaction (2012)
  • Author(s): Terami, Y., Yoshihara, A., Morimoto, K., Takata, G.
  • Organizer: Bioactive Okayama 2012
  • Place of Presentation: 岡山大学
• [Presentation] 微生物反応および酵素反応を用いたD-プシコースからD-タロースの生産 (2012)
  • Author(s): 寺見優司、吉原明秀、森本兼司、高田悟郎
  • Organizer: 日本生物工学会2012年度全国大会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Bacillus sp. K44株の希少糖に対する反応性と代謝経路の解明 (2012)
  • Author(s): 森本兼司、高田悟郎、小林秀明、吉原明秀
  • Organizer: 日本生物工学会2012年度全国大会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Mesorhizobium loti tono由来のD-タガトース3-エピメラーゼの機能解析 (2012)
  • Author(s): 上地敬子、深井善範、吉原明秀、森本兼司、高田悟郎
  • Organizer: 日本生物工学会2012年度全国大会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Cloning and characterization of mutant L-ribulose 3-epimerase for production of rare sugars from Mesorhizobium loti (2011)
  • Author(s): Keiko Uechi; Akihide Yoshihara; Kenji Morimoto; Goro Takata; Ken Izumori
  • Organizer: The 5th Symposium of International Society of Rare Sugar
  • Place of Presentation: 香川県
  • Year and Date: 2011-11-11
• [Presentation] X-Ray Structures of the Enzymes used for Rare Sugar Production and their Catalytic Reaction Mechanisms (2011)
  • Author(s): Hiromi Yoshida; Mitsugu Yamada; Kosei Takeda; Takeyori Nishitani; Yuya Ohyama; Masatsugu Yamaji; Tbmohiko Ishii; Goro Takata; Ken Izumori; Shigehiro Kamitori
  • Organizer: The 5th Symposium of International Society of Rare Sugar
  • Place of Presentation: 香川県
  • Year and Date: 2011-11-09
• [Presentation] Mesorhizobium lotiの生産するL-ラムノースイソメラーゼの基質特異性と触媒効率 (2010)
  • Author(s): 高田悟郎, 他
  • Organizer: 平成22年度 日本生物工学会大会
  • Place of Presentation: ワールドコンベンションセンター(宮崎県)