| Project/Area Number |
22580090
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
KAWAGUCHI Takashi 大阪府立大学, 大学院・生命環境科学研究科, 教授 (70195056)
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| Co-Investigator(Kenkyū-buntansha) |
SUMITANI Jun-ichi 大阪府立大学, 大学院・生命環境科学研究科, 准教授 (10264813)
TANI Shuji 大阪府立大学, 大学院・生命環境科学研究科, 助教(現講師) (80405357)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 微生物利用学 / セルラーゼ / β-グルコシダーゼ / バイオマス / A. aculeatus / 進化分子工学 / A.aculeatus |
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| Research Abstract |
We performed to improve the ・-glucosidase 1 which was the most useful enzyme among cellulase components produced by a filamentous fungus, Aspergillus aculeatus, for the saccharification of cellulosic biomass. Firstly, the activity against insoluble cellulose of relatively high molecular mass was raised by adding the cellulose-biding domain. Secondary, a mutantenzyme with drastically lowered product inhibition by glucose was successfully isolated after the screening of a random mutagenesis library on amino acid residues around the substrate-binding site.
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