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Comprehensive functional analysis offungal pectin-degrading enzymes

Research Project

Project/Area Number 22580091
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionOsaka Prefecture University

Principal Investigator

SAKAMOTO Tatsuji  大阪府立大学, 生命環境科学研究科, 准教授 (10275282)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords微生物酵素 / バイオマス / ペクチン / Penicillium chrysogenum / アラビノフラノシダーゼ / アラビナナーゼ / ガラクトシダーゼ / ガラクタナーゼ / フェルラ酸エステラーゼ / ラムノガラクチュロナン分解酵素 / Penicillin chrysogenum
Research Abstract

An important component of a recycling-based society is the production of high-value-added substances from unutilized biomass, such as non-edible food parts generated by the food processing industries. The present study focused on pectin, a major unutilized biomass, and its efficient degradation by fungi. Because of pectin's complex structure, a number of enzymes are required for its degradation. Thirty pectinolytic enzymes were characterized in this study

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (28 results)

All 2013 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (19 results) Book (2 results)

  • [Journal Article] Peculiarities and applications of galactanolytic enzymes that act on type I and II arabinogalactans2013

    • Author(s)
      Sakamoto T, Ishimaru M
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: (in press) Issue: 12 Pages: 5201-5213

    • DOI

      10.1007/s00253-013-4946-2

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical characterization of a GH53 endo-・-1,4-galactanase and a GH35 exo-・-1,4-galactanase from Penicillium chrysogenum2013

    • Author(s)
      Sakamoto T, Nishimura Y, 他
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 97 Issue: 7 Pages: 2895-2906

    • DOI

      10.1007/s00253-012-4154-5

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Substrate specificity and gene expression of two Penicillium chrysogenumα-L-arabinofuranosidases (AFQ1 and AFS1) belonging to glycoside hydrolase families 51 and 542013

    • Author(s)
      Sakamoto T, Inui M, 他
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 97 Issue: 3 Pages: 1121-1130

    • DOI

      10.1007/s00253-012-3978-3

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical characterization and gene expression of two endo-arabinanases from Penicillium chrysogenum31B2012

    • Author(s)
      Sakamoto T, Inui M, 他
    • Journal Title

      Appl Microbiol Biotechnol.

      Volume: 93 Issue: 3 Pages: 1087-1096

    • DOI

      10.1007/s00253-011-3452-7

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-resolution structure of exo-arabinanase from Penicillium chrysogenum.2012

    • Author(s)
      Sogabe Y, Kitatani T, 他
    • Journal Title

      Acta Crystallogr D Biol Crystallogr

      Volume: 67 Issue: 5 Pages: 415-422

    • DOI

      10.1107/s0907444911006299

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a GH62 α-L-arabinofuranosidase specific for arabinoxylan produced by Penicillium chrysogenum2011

    • Author(s)
      Sakamoto T, Ogura A, 他
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 90 Issue: 1 Pages: 137-146

    • DOI

      10.1007/s00253-010-2988-2

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a GH62 α-L-arabinofuranosidase specific for arabinoxylan produced by Penicillium chrysogenum.

    • Author(s)
      T.Sakamoto, A.Ogura, M.Inui, S.Tokuda, S.Hosokawa, H.Ihara, N.Kasai
    • Journal Title

      Appl.Microbiol.Biotechnol.

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] enicillium chrysogenum31B株由来の L-アラビナン特異的α-L-アラビノフラノシダーゼ2012

    • Author(s)
      篠崎文香
    • Organizer
      日本応用糖質科学会大会
    • Place of Presentation
      東京
    • Year and Date
      2012-09-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 不溶性小麦アラビノキシランの分解に適した酵素の選択2012

    • Author(s)
      楠本敬文
    • Organizer
      日本応用糖質科学会大会
    • Place of Presentation
      東京
    • Year and Date
      2012-09-20
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Penicillium chrysogenum由来 3 種のβ-ガラクトシダーゼの反応特性解析2012

    • Author(s)
      西村勇一
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-25
    • Related Report
      2012 Final Research Report
  • [Presentation] Penicillium chrysogenum由来3種のβ-ガラクトシダーゼの反応特性解析2012

    • Author(s)
      西村勇一、笠井尚哉、阪本龍司
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都)
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Penicillium chrysogenum 31B株由来のL-アラビナン特異的α-L-アラビノフラノシダーゼ2012

    • Author(s)
      篠﨑文香
    • Organizer
      日本応用糖質科学会大会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] Penicillium chrysogenumの有する 5 種の推定エンドアラビナナーゼ遺伝子の機能解析と発現様式2011

    • Author(s)
      川上拓也
    • Organizer
      日本応用糖質科学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Penicillium chrysogenumの有する5種の推定エンドアラビナナーゼ遺伝子の機能解析と発現様式2011

    • Author(s)
      川上拓也、徳田沙耶佳、笠井尚哉, 阪本龍司
    • Organizer
      日本応用糖質科学会大会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 中温性アラビナン分解酵素の発現・精製2011

    • Author(s)
      小堀洋平
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Penicillium chrysogenum由来3種のα-L-アラビノフラノシダーゼの反応特性と遺伝子発現解析2011

    • Author(s)
      細川幸子
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Penicillium chrysogenum由来3種のα-L-アラビノフラノシダーゼの反応特性と遺伝子発現解析2011

    • Author(s)
      細川幸子、徳田沙耶佳、笠井尚哉、阪本龍司
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都)(発表確定)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 中温性アラビナン分解酵素の発現・精製2011

    • Author(s)
      小堀洋平、秋吉美歌、阪本龍司、多田俊治
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都)(発表確定)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 中温性エンド型アラビナナーゼ(AbnS1)の構造2010

    • Author(s)
      小堀洋平
    • Organizer
      日本結晶学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2010-12-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 中温性エンド型アラビナナーゼ(AbnS1)の構造2010

    • Author(s)
      小堀洋平、秋吉美歌、阪本龍司、多田俊治
    • Organizer
      日本結晶学会大会
    • Place of Presentation
      大阪大学(大阪)
    • Year and Date
      2010-12-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Penicillium chrysogenumの有する 8 種の推定アラビノフラノシダーゼ遺伝子の機能解析と発現様式2010

    • Author(s)
      阪本龍司
    • Organizer
      食品酵素化学研究会第10回学術講演会
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-04
    • Related Report
      2012 Final Research Report
  • [Presentation] 好冷性酵素・エンド型アラビナナーゼの構造2010

    • Author(s)
      多田俊治
    • Organizer
      食品酵素化学研究会第10回学術講演会
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-04
    • Related Report
      2012 Final Research Report
  • [Presentation] Penicillium chrysogenumの有する8種の推定アラビノフラノシダーゼ遺伝子の機能解析と発現様式2010

    • Author(s)
      阪本龍司、細川幸子、徳田沙耶佳、笠井尚哉
    • Organizer
      食品酵素化学研究会
    • Place of Presentation
      大阪府立大学(大阪)
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] 好冷性酵素・エンド型アラビナナーゼの構造2010

    • Author(s)
      多田俊治、秋吉美歌、曽我部祐里、山口亜佐子、阪本龍司
    • Organizer
      食品酵素化学研究会
    • Place of Presentation
      大阪府立大学(大阪)
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Penicillium chrysogenumの有する 8 種の推定アラビノフラノシダーゼ遺伝子の発現プロファイルと機能解析2010

    • Author(s)
      細川幸子
    • Organizer
      日本農芸化学会関西支部例会
    • Place of Presentation
      大阪
    • Year and Date
      2010-07-04
    • Related Report
      2012 Final Research Report
  • [Presentation] Penicillium chrysogenumの有する8種の推定アラビノフラノシダーゼ遺伝子の発現プロファイルと機能解析2010

    • Author(s)
      細川幸子、徳田沙耶佳、笠井尚哉、阪本龍司
    • Organizer
      日本農芸化学会関西支部例会
    • Place of Presentation
      大阪府立大学(大阪)
    • Year and Date
      2010-07-04
    • Related Report
      2010 Annual Research Report
  • [Book] バイオマス分解酵素研究の最前線-セルラーゼ・ヘミセルラーゼを中心として2012

    • Author(s)
      阪本龍司
    • Publisher
      シーエムシー
    • Related Report
      2012 Final Research Report
  • [Book] ペクチンの構造と分解酵素」の項分担2011

    • Author(s)
      阪本龍司
    • Publisher
      シーエムシー出版、 「食品酵素化学の最新技術と応用II-展開するフードプロテオミクス
    • Related Report
      2012 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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