Generation of the cell factories for bio-based organic acid production through improvement of filamentous fungi by metabolic engineering
| Project/Area Number |
22580093
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 応用微生物 / 酵素 / セルファクトリ / 代謝工学 / 有機酸 / クエン酸 / シュウ酸 / トランスアコニット酸 / 発酵 / バイオテクノロジー / クロコウジカビ / ポリケタイド |
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| Research Abstract |
For generation of cell factories for bio-based organic acid production, generation of strains showing high gene-targeting frequencies was performed in citric acid-producing Aspergillus niger. Novel type III polyketide synthase and some key enzymes, such as methylcitrate synthase and aconitate isomerase, in relation to the tricarboxylic acid cycle were examined through gene cloning and enzymatic characterization. There is some possibility that heterologous expression of genes encoding novel and specific enzymes in the citric acid-producing strains as hosts and improvement by metabolic engineering will open the way for generation of the cell factories for bio-based organic acid production.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Production of p-Aminosalicylic Acid through Enzymatic Kolbe-Schmitt Reaction Catalyzed by Reversible Salicylic Acid Decarboxylase.2011
Author(s)
Kirimura, K., Yanaso, S., Kosaka, S., Koyama, K., Hattori, T., Ishii, I.
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Journal Title
Chem. Lett.
Volume: 40
Pages: 206-208
Related Report
Peer Reviewed
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