Conversion of hydrolases into transglycosidases
| Project/Area Number |
22580099
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
MORI Haruhide 北海道大学, 大学院・農学研究院, 准教授 (80241363)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 酵素化学 / 糖転移 / 酵素反応機構 / オリゴ糖 / イソマルトオリゴ糖 |
|---|
| Research Abstract |
A variety of oligosaccharides can be produced by carbohydrate hydrolases. In this work, amino acid residues responsible for high transglycosylation activities were identified through the comparison of transglycosidases with hydrolases. It was clarified that one transglycosidase had a catalytic domain similar to anomer-inverting hydrolases, which possess no transglycosidase activity.
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Report
(4 results)
Research Products
(65 results)
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[Journal Article] α-Glucosidase-catalyzed novel reaction on 1,5-anhydrofructose, suggesting new metabolic pathway for production of glucose from starch2012
Author(s)
Kim YM, Saburi W, Yu S, Nakai H, Maneesan J, Kang MS, Chiba S, Kim D, Okuyama M, Mori H, Kimura A
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Journal Title
J Biol Chem
Volume: 287(27)
Issue: 27
Pages: 22441-22444
DOI
Related Report
Peer Reviewed
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