Biological function and gene regulation of epididymal-specific carboxylesterase (CES4)

• Project/Area Number: 22580100
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Iwate University
• Principal Investigator: YAMASHITA Tetsuro 岩手大学, 農学部, 准教授 (20202377)
• Co-Investigator(Kenkyū-buntansha): MORIMATSU Masami 北海道大学, 遺伝子病制御研究所, 准教授 (70241370)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 精巣上体 / エステラーゼ / 酵素 / カルボキシルエステラーゼ / 遺伝子変異マウス / 巣上体 / KOマウス

Research Abstract

The biological function of an epididymal-specific carboxylesterase, CES4, was studied using Ces4 knock out mouse. The metabolome analysis of epididymal cell extracts revealed that the most of the glutathione was identified as oxidative form in the epididymis of KO mouse. The expression of oxidative stress-responsive heme oxygenase-1 (HO-1) was also increased in the KO mouse tissue. These data indicated that the epididymis of KO mouse was exposed by oxidative stress.

Research Products

• [Journal Article] Characterisation of the carboxylesterase enzyme cauxin in the seminal fluid of the cat (2011)
  • Author(s): Miyazaki, M., Miyazaki, T., Toyonaga, M., Tsutsui, T., Taira, H., Yamashita, T. and Suzuki, A.
  • Journal Title: The Veterinary Journal Volume: Vol. 190 Pages: 378-382
  • Peer Reviewed
• [Journal Article] Characterisation of the carboxylesterase enzyme cauxin in the seminal fluid of the cat (2011)
  • Author(s): Yamashita, T.
  • Journal Title: Vet.J. Volume: (印刷中) Issue: 3 Pages: 378-382
  • DOI: 10.1016/j.tvjl.2010.11.014
  • Peer Reviewed
• [Journal Article] Recommended nomenclature for five mammalian carboxylesterase gene families: human, mouse, and rat genes and proteins (2010)
  • Author(s): Holmes, R., Wright, M., Laulederkind, S., Cox, L., Hosokawa, M., Imai, T., Ishibashi, S., Lehner, R., Miyazaki, M., Perkins, E., Potter, P., Redinbo, M., Robert, J., Satoh, T., Yamashita, T., Yan, B., Yokoi, T., Zechner, R. and Maltais, L. J.
  • Journal Title: Mammalian Genome Volume: Vol. 21 Pages: 427-441
  • Peer Reviewed
• [Journal Article] Recommended nomenclature for five mammaian carboxylesterase gene families human, mouse and rat genes and proteins. (2010)
  • Author(s): Roger S Holmes, Tetsurou Yamashita, et al.
  • Journal Title: Mamm.Genome Volume: 21 Pages: 427-441
  • Peer Reviewed
• [Presentation] 遺伝子欠損マウスを用いた精巣特異的カルボキシルエステラーゼ(CES4)の機能解析 (2012)
  • Author(s): 山下哲郎、金成瑞穂、松舘光人、宮崎雅雄、森松正美
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-16
• [Presentation] ネコ尿タンパク質コーキシンと尿臭生成機構 (2012)
  • Author(s): 山下哲郎
  • Organizer: 日本獣医師会獣医学術学会年次大会
  • Place of Presentation: 札幌市
  • Year and Date: 2012-02-03
• [Presentation] 遺伝子欠損マウスを用いた精巣特異的カルボキシルエステラーゼ(CES4)の機能解析 (2012)
  • Author(s): 山下哲郎
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] ネコ尿タンパク質コーキシンと尿臭生成機構 (2011)
  • Author(s): 山下哲郎
  • Organizer: 平成23年度日本獣医師会獣医学術学会年次大会(招待講演)
  • Place of Presentation: 札幌コンベンションセンター (北海道)
  • Year and Date: 2011-02-03