A study of controlling splicing for conditional gene expression.

• Project/Area Number: 22580101
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Tokai University
• Principal Investigator: SASAGAWA Noboru 東海大学, 工学部, 准教授 (70302817)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 遺伝子発現 / 遺伝子発現調節 / スプライシング / RNA 結合タンパク質 / エキソン / イントロン / RNA結合タンパク質

Research Abstract

The aim of this study is to control alternative splicing and gene expression as our own purpose. By using nematode C.elegans as model organism, reporter mini-gene with GFP/RFP is constructed. This mini-gene is designed to express GFP/RFP only when alternative splicing is completed as desired. An RNA binding protein which plays an important role to alternative splicing is also investigated.

Research Products

• [Journal Article] Alternative splicing of myomesin 1 gene is aberrantly regulated in myotonic dystrophy type 1 (2011)
  • Author(s): Koebis M., Ohsawa N., Kino Y., Sasagawa N., Nishino I. and Ishiura S.
  • Journal Title: Genes to Cells Volume: 16 Pages: 961-972
• [Journal Article] Transgenic Rice Expressing Amyloid β-peptide for Oral Immunization (2011)
  • Author(s): Yoshida T., Kimura E., Koike S., Nojima J., Futai E., Sasagawa N., Watanabe Y. and Ishiura S.
  • Journal Title: Int. J. Biol. Sci Volume: 7 Pages: 301-307
• [Journal Article] Production of Anti-Amyloid β Antibodies in Mice Fed Rice Expressing Amyloid β (2011)
  • Author(s): Nojima J., Ishii-Katsuno R., Futai E., Sasagawa N., Watanabe Y., Yoshida T. and Ishiura S
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 75 Pages: 396-400
  • NAID: 10027898296
• [Journal Article] Production of Anti-Amyloid β Antibodies in Mice Fed Rice Expressing Amyloid β (2011)
  • Author(s): Nojima J, Ishii-Katsuno R, Futai E, Sasagawa N, Watanabe Y, Yoshida T, Ishiura S
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 396-400
  • NAID: 10027898296
  • Peer Reviewed
• [Journal Article] Reduction of amyloid beta-peptide accumulation in Tg2576 transgenic mice by oral vaccination (2010)
  • Author(s): Ishii-Katsuno R., Nakajima A., Katsuno T., Nojima J., Futai E., Sasagawa N., Yoshida T., Watanabe Y. and Ishiura S.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 399 Pages: 593-599
• [Journal Article] ADAM19 autolysis is activated by LPS and promotes non-classical secretion of cysteine-rich protein 2 (2010)
  • Author(s): Tanabe C., Hotoda N., Sasagawa N., Futai E., Komano H. and Ishiura S
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 396 Pages: 927-32
• [Journal Article] Reduction of amyloid beta-peptide accumulation in Tg2576 transgenic mice by oral vaccination. (2010)
  • Author(s): Ishii-Katsuno R, Nakajima A, Katsuno T, Nojima J, Futai E, Sasagawa N, Yoshida T, Watanabe
  • Journal Title: Biochem.Biophys.Res.Comm. Volume: 399 Pages: 593-599
  • Peer Reviewed
• [Journal Article] ADAM19 autolysis is activated by LPS and promotes non-classical secretion of cysteine-rich protein 2. (2010)
  • Author(s): Tanabe C, Hotoda N, Sasagawa N, Futai E, Komano H, Ishiura S
  • Journal Title: Biochem.Biophys.Res.Comm. Volume: 396 Pages: 927-932
  • Peer Reviewed
• [Presentation] 線虫C.elegans のRNA結合タンパク質であるCeMBL 遺伝子の機能解析 (2013)
  • Author(s): 鈴木俊輔、笹川昇
  • Organizer: 日本農芸化学会2013 年度年会
  • Place of Presentation: 仙台
  • Year and Date: 2013-03-27
• [Presentation] ブドウ抽出物ANOX-R5による線虫C.elegans の寿命への影響 (2013)
  • Author(s): 高橋航大、笹川昇
  • Organizer: 日本農芸化学会2013年度年会
  • Place of Presentation: 仙台
  • Year and Date: 2013-03-27
• [Presentation] RNAi実験を用いた、線虫C.elegansのRNA結合タンパク質であるCeMBL遺伝子の選択的スプライシング発現産物が持つ機能の追究 (2012)
  • Author(s): 鈴木俊輔、笹川昇
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] RNAi実験を用いた、線虫C.elegansのRNA結合タンパク質であるCeMBL遺伝子の選択的スプライシング発現産物が持つ機能の追究 (2012)
  • Author(s): 鈴木俊輔、笹川昇
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都(京都女子大学)
  • Year and Date: 2012-03-23
• [Presentation] アルツハイマー病食物ワクチンのためのアミロイドベータペプチドを含有した遺伝子改変米の作出 (2010)
  • Author(s): 吉田泰二, 山岸紀子, 木村映一, 小池説夫, 野嶋純, 二井勇人, 笹川昇, 渡辺雄一郎, 石浦章一
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] 線虫C.elegans のRNA 結合タンパク質であるCeMBL 遺伝子の機能解析
  • Author(s): 鈴木俊輔（笹川昇）
  • Organizer: 日本農芸化学会2013 年度大会
  • Place of Presentation: 東北大学
• [Presentation] ブドウ抽出物ANOX-R5 による線虫C.elegans の寿命への影響
  • Author(s): 高橋航大（笹川昇）
  • Organizer: 日本農芸化学会2013 年度大会
  • Place of Presentation: 東北大学