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A study of controlling splicing for conditional gene expression.

Research Project

Project/Area Number 22580101
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionTokai University

Principal Investigator

SASAGAWA Noboru  東海大学, 工学部, 准教授 (70302817)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords遺伝子発現 / 遺伝子発現調節 / スプライシング / RNA 結合タンパク質 / エキソン / イントロン / RNA結合タンパク質
Research Abstract

The aim of this study is to control alternative splicing and gene expression as our own purpose. By using nematode C.elegans as model organism, reporter mini-gene with GFP/RFP is constructed. This mini-gene is designed to express GFP/RFP only when alternative splicing is completed as desired. An RNA binding protein which plays an important role to alternative splicing is also investigated.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (15 results)

All 2013 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 3 results) Presentation (7 results)

  • [Journal Article] Alternative splicing of myomesin 1 gene is aberrantly regulated in myotonic dystrophy type 12011

    • Author(s)
      Koebis M., Ohsawa N., Kino Y., Sasagawa N., Nishino I. and Ishiura S.
    • Journal Title

      Genes to Cells

      Volume: 16 Pages: 961-972

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Journal Article] Transgenic Rice Expressing Amyloid β-peptide for Oral Immunization2011

    • Author(s)
      Yoshida T., Kimura E., Koike S., Nojima J., Futai E., Sasagawa N., Watanabe Y. and Ishiura S.
    • Journal Title

      Int. J. Biol. Sci

      Volume: 7 Pages: 301-307

    • Related Report
      2012 Final Research Report
  • [Journal Article] Production of Anti-Amyloid β Antibodies in Mice Fed Rice Expressing Amyloid β2011

    • Author(s)
      Nojima J., Ishii-Katsuno R., Futai E., Sasagawa N., Watanabe Y., Yoshida T. and Ishiura S
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 75 Pages: 396-400

    • NAID

      10027898296

    • Related Report
      2012 Final Research Report
  • [Journal Article] Production of Anti-Amyloid β Antibodies in Mice Fed Rice Expressing Amyloid β2011

    • Author(s)
      Nojima J, Ishii-Katsuno R, Futai E, Sasagawa N, Watanabe Y, Yoshida T, Ishiura S
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 75 Pages: 396-400

    • NAID

      10027898296

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduction of amyloid beta-peptide accumulation in Tg2576 transgenic mice by oral vaccination2010

    • Author(s)
      Ishii-Katsuno R., Nakajima A., Katsuno T., Nojima J., Futai E., Sasagawa N., Yoshida T., Watanabe Y. and Ishiura S.
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 399 Pages: 593-599

    • Related Report
      2012 Final Research Report
  • [Journal Article] ADAM19 autolysis is activated by LPS and promotes non-classical secretion of cysteine-rich protein 22010

    • Author(s)
      Tanabe C., Hotoda N., Sasagawa N., Futai E., Komano H. and Ishiura S
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 396 Pages: 927-32

    • Related Report
      2012 Final Research Report
  • [Journal Article] Reduction of amyloid beta-peptide accumulation in Tg2576 transgenic mice by oral vaccination.2010

    • Author(s)
      Ishii-Katsuno R, Nakajima A, Katsuno T, Nojima J, Futai E, Sasagawa N, Yoshida T, Watanabe
    • Journal Title

      Biochem.Biophys.Res.Comm.

      Volume: 399 Pages: 593-599

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ADAM19 autolysis is activated by LPS and promotes non-classical secretion of cysteine-rich protein 2.2010

    • Author(s)
      Tanabe C, Hotoda N, Sasagawa N, Futai E, Komano H, Ishiura S
    • Journal Title

      Biochem.Biophys.Res.Comm.

      Volume: 396 Pages: 927-932

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 線虫C.elegans のRNA結合タンパク質であるCeMBL 遺伝子の機能解析2013

    • Author(s)
      鈴木俊輔、笹川昇
    • Organizer
      日本農芸化学会2013 年度年会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] ブドウ抽出物ANOX-R5による線虫C.elegans の寿命への影響2013

    • Author(s)
      高橋航大、笹川昇
    • Organizer
      日本農芸化学会2013年度年会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] RNAi実験を用いた、線虫C.elegansのRNA結合タンパク質であるCeMBL遺伝子の選択的スプライシング発現産物が持つ機能の追究2012

    • Author(s)
      鈴木俊輔、笹川昇
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] RNAi実験を用いた、線虫C.elegansのRNA結合タンパク質であるCeMBL遺伝子の選択的スプライシング発現産物が持つ機能の追究2012

    • Author(s)
      鈴木俊輔、笹川昇
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都(京都女子大学)
    • Year and Date
      2012-03-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] アルツハイマー病食物ワクチンのためのアミロイドベータペプチドを含有した遺伝子改変米の作出2010

    • Author(s)
      吉田泰二, 山岸紀子, 木村映一, 小池説夫, 野嶋純, 二井勇人, 笹川昇, 渡辺雄一郎, 石浦章一
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 線虫C.elegans のRNA 結合タンパク質であるCeMBL 遺伝子の機能解析

    • Author(s)
      鈴木俊輔(笹川昇)
    • Organizer
      日本農芸化学会2013 年度大会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ブドウ抽出物ANOX-R5 による線虫C.elegans の寿命への影響

    • Author(s)
      高橋航大(笹川昇)
    • Organizer
      日本農芸化学会2013 年度大会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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