| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Research Abstract |
The (pro)renin receptor (PRR) is a single transmembrane receptor for the pressor enzyme renin and its inactive precursor prorenin. PRR induces the catalytic activation of prorenin along with intracellular signaling. In this study, the glutathione S transferase-fused extracellular domain of PRR expressed in mammalian cells was observed by immunofluorescence microscopy to be localized in the endoplasmic reticulum-like membranous structure, and recovered in the detergent phase in detergent-based two-phase separation experiments, suggesting retention inside the cell through stable membrane association. By co-immunoprecipitation of FALG-tagged PRR with its interacting proteins in human cultured cells and mass spectrometry, GRP78/BiP was identified as a novel binding protein for PRR.
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