Analysis of hibernation-associated gene regulation in chipmunk

• Project/Area Number: 22580107
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kitasato University
• Principal Investigator: TAKAMATSU Nobuhiko 北里大学, 理学部, 教授 (40206876)
• Co-Investigator(Kenkyū-buntansha): KODERA Yoshio 北里大学, 理学部, 准教授 (60265733)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 冬眠 / 転写制御 / 概年リズム / 肝臓 / 転写因子 / 概念リズム / プロテオーム

Research Abstract

Mammalian hibernation is a seasonal phenomenon with a circannual rhythm, and molecular mechanisms that govern hibernation-associated gene regulation in a circannual manner are still unknown. In the chipmunk, a mammalian hibernator, HSP70 gene transcription in the liver is activated in nonhibernating animals, and repressed in hibernating animals. In this study, we analyzed the hibernation-associated transcriptional regulation of the HSP70 gene, and revealed that the protein levels of HSF-1 and HSF-2 oscillate between the nonhibernating and hibernating seasons, resulting in the hibernation-associated transcriptional regulation of the HSP70 gene.

Research Products

• [Journal Article] Identification of bovine hibernation-specific protein complex and evidence of its regulation in fasting and aging (2013)
  • Author(s): Fujita, S., Okamoto, R., Taniguchi, M., Ban-Tokuda, T., Konishi, K., Goto, I., Yamamoto, Y., Sugimoto, K., Takamatsu, N., Nakamura, M., Shiraki, K., Buechler, C., Ito, M.
  • Journal Title: J. Biochem Volume: 153巻 Issue: 5 Pages: 453-461
  • DOI: 10.1093/jb/mvt008
  • Peer Reviewed
• [Journal Article] Identification of bovine hibernation-specific protein complex and evidence of its regulation i fasting and aging. (2013)
  • Author(s): Fujita, S.
  • Journal Title: J. Biochem. Volume: 153 Pages: 453-461
  • Peer Reviewed
• [Presentation] シマリスHSP70遺伝子のHSF-2による冬眠に伴う転写調節機構の解析 (2012)
  • Author(s): 飯尾浩子,伊藤道彦,高松信彦
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-12-13
• [Presentation] Hibernation-associated transcriptional regulation of the chipmunk HP-25 gene (2011)
  • Author(s): 菅井玲,綿引陽子,廣瀬真一,伊藤道彦,高松信彦
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-12-15
• [Presentation] Hibernation-associated transcriptional regulation of the chipmunk HP-25 gene (2011)
  • Author(s): 菅井玲, 他4名
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-15
• [Presentation] 哺乳動物の冬眠にともなう肝臓のプロテオーム変動の解析 (2010)
  • Author(s): 松澤彰彦,川島祐介,斎藤達也,大橋和也,高松信彦,小寺義男
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-09
• [Presentation] 哺乳動物の冬眠にともなう肝臓のプロテオーム変動の解析 (2010)
  • Author(s): 松澤彦, ら6名
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] シマリスHSP70遺伝子のHSF-2による冬眠に伴う転写調節機構の解析
  • Author(s): 飯尾浩子
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡
• [Remarks] ホームページ
  • URL: http://www.kitasato-u.ac.jp/sci/resea/seibutsu/joho/index.htm