Toxicity and antitumor effects of parasporin-4 from Bacillus thuringiensis strain A1470
| Project/Area Number |
22580111
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Fukuoka Industrial Technology Center |
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Principal Investigator |
OKUMURA Shiro 福岡県工業技術センター, 生物食品研究所, 専門研究員 (40399671)
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| Co-Investigator(Renkei-kenkyūsha) |
SAITOH Hiroyuki 福岡県工業技術センター, 生物食品研究所, 専門研究員 (60416493)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 生物工学 / パラスポリン / 変異マウス / 大腸がん / Bacillus thuringiensis / 微生物 / 癌 / モデルマウス / タンパク質 / 蛋白質 |
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| Research Abstract |
Parasporin-4 (PS4) is a cytotoxic protein produced by Bacillus thuringiensis A1470 strain. It exhibits specific cytotoxicity against human cancer cell lines especially CACO-2, Sawano, and MOLT-4 cells. And it does not exhibit the activity against 4 normal tissue cells such as human primary hepatocyte cells, UtSMC, MRC-5, and normal T cells. However, these results do not provide assurance that the protein exhibits no toxicity to mammals. In this study, we determined acute toxicity and effects of long-term administration of PS4 on mouse. In addition, we tested the inhibitory effects of oral administration of pre-PS4 against colon cancer on mutant mice.
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Report
(5 results)
Research Products
(18 results)
