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Selective control of plant P450 enzymes: Development of small molecules regulating plant growth and production of sweet substances

Research Project

Project/Area Number 22580118
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionShizuoka University

Principal Investigator

TODOROKI Yasushi  静岡大学, 農学部, 教授 (30324338)

Co-Investigator(Kenkyū-buntansha) OHNISHI Toshiyuki  静岡大学, 若手グローバル研究リーダー育成拠点, 特任助教 (60542165)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords植物成長調節物質 / シトクロムP450酵素 / 阻害剤 / ジベレリン / アブシジン酸 / 乾燥耐性 / 植物P450 / ジベレリン生合成 / 甘味物質 / アゾール化合物
Research Abstract

This study has developed the three-types of selective inhibitors of cytochrome P450 enzymes involved in biosynthesis of gibberellin that promotes seed germination and stem elongation, catabolic inactivation of abscisic acid that antagonizes gibberellin-promoted seed germination and plays an essential role in adaptive stress responses, and biosynthesis of stevioside, a sweet substance of Stevia. These inhibitors are expected to be a useful tool to control plant growth and production of sweet substances.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (17 results)

All 2012 2011 2010

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (13 results)

  • [Journal Article] A conformationally restricted uniconazole analogue as a specific inhibitor of rice ent-kaurene oxidase CYP701A62012

    • Author(s)
      Todoroki, Y.; Naiki, K.; Muramatsu, T.; Ohnishi, T.; Ueno, K., Mizutani, M.; Hirai, N
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 22 Issue: 9 Pages: 3240-3243

    • DOI

      10.1016/j.bmcl.2012.03.028

    • NAID

      120004226429

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Abscinazole-E2B, a practical and selective inhibitor of ABA 8'-hydroxylase CYP707A2012

    • Author(s)
      Okazaki, M.; Kittikorn, M.; Ueno, K.; Mizutani, M.; Hirai, N.; Kondo, S.; Ohnishi, T.; Todoroki, Y
    • Journal Title

      Bioorg. Med. Chem

      Volume: 20 Issue: 10 Pages: 3162-3172

    • DOI

      10.1016/j.bmc.2012.03.068

    • NAID

      120004226430

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A conformationally restricted uniconazole analogue as a specific inhibitor of rice ent-kaurene oxidase, CYP701A62012

    • Author(s)
      Y. Todoroki
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 22 Issue: 18 Pages: 3240-3243

    • DOI

      10.1016/j.bmcl.2010.07.067

    • NAID

      120004226429

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Abscinazole-E1, a novel chemical tool for exploring the role of ABA 8'-hydroxylase CYP707A2011

    • Author(s)
      Okazaki, M.; Nimitkeatkai, H.; Muramatsu, T.; Aoyama, H.; Ueno, K.; Mizutani, M.; Hirai, N.; Kondo, S.; Ohnishi, T.; Todoroki, Y.
    • Journal Title

      Bioorg. Med. Chem

      Volume: 19 Issue: 1 Pages: 406-413

    • DOI

      10.1016/j.bmc.2010.11.011

    • NAID

      120002829526

    • Related Report
      2012 Final Research Report
  • [Presentation] ジベレリン代謝に関わる CYP714 ファミリーの阻害剤2012

    • Author(s)
      新間優子,酒井杏奈,内記久美,水谷正治,平井伸博,大西利幸,轟 泰司
    • Organizer
      日本農芸化学会 2012 年度大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-24
    • Related Report
      2012 Final Research Report
  • [Presentation] ジベレリン代謝に関わるCYP714ファミリーの阻害剤2012

    • Author(s)
      新間優子, 酒井杏奈, 内記久美, 水谷正治, 平井伸博, 大西利幸, 轟泰司
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学(京都府)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] ABA 8'-水酸化酵素選択的阻害剤abscinazole-E2B の光学活性体2011

    • Author(s)
      岡崎真理子,Hataitip Nimitkeatkai,近藤 悟,水谷正治,平井伸博,大西利幸,轟泰司
    • Organizer
      植物化学調節学会第46回大会
    • Place of Presentation
      宇都宮
    • Year and Date
      2011-11-01
    • Related Report
      2012 Final Research Report
  • [Presentation] ジベレリン生合成酵素CYP701A 選択的阻害剤2011

    • Author(s)
      内記久美,大西利幸,水谷正治,上野琴巳,平井伸博,轟 泰司
    • Organizer
      植物化学調節学会第46回大会
    • Place of Presentation
      宇都宮
    • Year and Date
      2011-11-01
    • Related Report
      2012 Final Research Report
  • [Presentation] ジベレリン生合成酵素CYP701A選択的阻害剤2011

    • Author(s)
      内記久美, 大西利幸, 水谷正治, 上野琴巳, 平井伸博, 轟泰司
    • Organizer
      植物化学調節学会第46回大会
    • Place of Presentation
      宇都宮大学(栃木県)
    • Year and Date
      2011-11-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] ジベレリン生合成における13位水酸化反応を阻害する物質2011

    • Author(s)
      酒井杏奈, 新間優子, 大西利幸, 水谷正治, 平井伸博, 轟泰司
    • Organizer
      植物化学調節学会第46回大会
    • Place of Presentation
      宇都宮大学(栃木県)
    • Year and Date
      2011-11-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] アブシジン酸代謝不活性化酵素 CYP707Aの選択的阻害剤アブシナゾール E2B2011

    • Author(s)
      岡崎真理子,村松 卓,青山 光,上野琴巳,水谷正治,平井伸博,Hataitip Nimitkeatkai,近藤 悟,大西利幸,轟 泰司
    • Organizer
      日本農芸化学会 2011 年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-26
    • Related Report
      2012 Final Research Report
  • [Presentation] ジベレリン生合成に関わる P450 酵素の選択的阻害剤2011

    • Author(s)
      内記 久美,村松 卓,大西利幸,水谷正治,上野琴巳,平井伸博,轟 泰司
    • Organizer
      日本農芸化学会 2011 年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-26
    • Related Report
      2012 Final Research Report
  • [Presentation] ジベレリン生合成に関わるP450酵素の選択的阻害剤2011

    • Author(s)
      内記 久美
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学(京都市)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] ジベレリン生合成酵素選択的 P450 阻害剤のスクリーニング2010

    • Author(s)
      村松 卓,大西利幸,水谷正治,上野琴巳,平井伸博,轟 泰司
    • Organizer
      植物化学調節学会第45回大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-11-02
    • Related Report
      2012 Final Research Report
  • [Presentation] ABA 8'-水酸化酵素に対する実用性の高い選択的阻害剤アブシナゾール E2B2010

    • Author(s)
      岡崎真理子,村松 卓,青山 光,上野琴巳,水谷正治,平井伸博,Hataitip Nimitkeatkai,近藤 悟,大西利幸,轟 泰司
    • Organizer
      植物化学調節学会第45回大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-11-02
    • Related Report
      2012 Final Research Report
  • [Presentation] ジベレリン生合成酵素選択的P450阻害剤のスクリーニング2010

    • Author(s)
      村松卓
    • Organizer
      植物化学調節学会第45回大会
    • Place of Presentation
      神戸大学百年記念館(神戸市)
    • Year and Date
      2010-11-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] CYP707A 特異的阻害剤アブシナゾールの創出 抄録集 p.1142010

    • Author(s)
      岡崎真理子,青山 光,内記久美,村松 卓,上野琴巳,水谷正治,平井伸博,大西利幸,轟 泰司
    • Organizer
      日本ケミカルバイオロジー学会第5回年会
    • Place of Presentation
      横浜
    • Year and Date
      2010-05-19
    • Related Report
      2012 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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