Development of a new probe of a flavonoid metabolite, DOPAC, for understanding the biomolecule modification
Project/Area Number |
22580129
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Food science
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Research Institution | Okayama University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KATO Yoji 兵庫県立大学, 環境人間学部, 教授 (30305693)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | ポリフェノール / ケルセチン / 生体内代謝物 / 分子標的 / DOPAC / クリックケミストリー / ビオチンラベル化 / EGCG / 抗酸化作用 / アポトーシス |
Research Abstract |
The interest in fermented products in the large intestine such as a quercetin glucoside metabolite, 3,4-dihydroxyphenylacetic acid (DOPAC), has started to grow rapidly. In this study, we identified several plausible target molecules for polyphenols by understanding the molecular mechanisms underlying biological activities related to their chemical characteristics. We also revealed that DOPAC exhibited radical scavenging and phase II drug metabolizing enzyme-inducing activities more potently than other quercetin metabolites. Furthermore, we developed a new probe (DOPAC propargyl ester) to detect target proteins and confirmed its applicability to “click chemistry”.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Cytotoxicity of benzyl isothiocyanate in normal renal proximal tubular cells and its modulation by glutathione.2012
Author(s)
Abe, N., Okuhira, M., Tsutsui, C., Shimoishi, Y., Murata, Y., Nakamura, Y
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Journal Title
J. Agric. Food Chem
Volume: 60(7)
Issue: 7
Pages: 1887-1892
DOI
Related Report
Peer Reviewed
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[Journal Article] Luteolin overcomes resistance to benzyl isothiocyanate-induced apoptosis in human colorectal cancer HCT-116 cells2012
Author(s)
Sakai, R., Yokobe, S., Abe, N., Miyoshi, N., Murata, Y., Nakamura, Y
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Journal Title
J. Food Drug Anal
Volume: 20(Suppl.)
Pages: 389-393
Related Report
Peer Reviewed
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[Journal Article] Involvement of intracellular oxidative stress-sensitive pathway in phloxine B-induced photocytotoxicity in human T lymphocytic leukemia cells.2012
Author(s)
Qi, H., Zhu, B., Abe, N., Shin, Y., Murata, Y., Nakamura, Y
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Journal Title
Food Chem. Toxicol
Volume: 50(6)
Issue: 6
Pages: 1841-1847
DOI
Related Report
Peer Reviewed
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[Journal Article] Development and application of a method for identification of isothiocyanate-targeted molecules in colon cancer cells2012
Author(s)
Miyoshi, N., Yonemochi, T., Tomono, S., Fukutomi, R., Nakamura, Y., Ohshima, H.
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Journal Title
Anal. Biochem.
Volume: 429(2)
Issue: 2
Pages: 124-131
DOI
Related Report
Peer Reviewed
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[Journal Article] Hydrogen peroxide-dependent photocyto -toxicity by phloxine B, a xanthene food colorant2011
Author(s)
Qi, H., Takano, H., Kato, Y., Wu, Q., Ogata, C., Zhu, B., Murata, Y., Nakamura, Y
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Journal Title
Biochim. Biophys. Acta Gen. Sub
Volume: 1810(7)
Issue: 7
Pages: 704-712
DOI
Related Report
Peer Reviewed
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[Journal Article] Localized cysteine sulfenic acid formation by vascular endothelial growth factor: Role in endothelial cell migration and angiogenesis2011
Author(s)
Kaplan, N., Urao, N., Furuta, E., Kim, S.J., Razvi, M., Nakamura, Y., McKinney, R.D., Poole, L.B., Fukai, T., Ushio-Fukai, M
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Journal Title
Free Radical Res
Volume: 45(10)
Issue: 10
Pages: 1124-1135
DOI
Related Report
Peer Reviewed
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[Journal Article] Artificial Food Colorants Inhibit Superoxide Production in Differentiated HL-60 Cells2010
Author(s)
Qi, H., Kawagishi, M., Yoshimoto, M., Takano, H., Zhu, B., Shimoishi, Y., Tada, M., Murata, Y., Nakamura, Y
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 74
Issue: 8
Pages: 1725-1728
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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