| Project/Area Number |
22580131
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Yamaguchi University |
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Principal Investigator |
KAJIYA Katsuko 山口大学, 大学院・医学系研究科, 大学院講師 (00379942)
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| Co-Investigator(Kenkyū-buntansha) |
岸 博子 山口大学, 医学(系)研究科(研究院), 准教授 (40359899)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 血管病 / 膜ラフト / モデル膜 / リポソーム / シグナル伝達 / 天然成分 |
|---|
| Research Abstract |
Abnormal vascular smooth muscle (VSM)contraction (vasospasm) is one of the crucial phenomena which cause cardiac angina and myocardial infarction. We previously found that sphingosylphosphorylcholine (SPC) as an upstream mediator for the abnormal pathway through the activation of Fyn, a member of Src family tyrosine kinase. The localization of Fyn in cholesterol-enriched membrane microdomain, lipid rafts, suggest the essential roles of membrane lipid rafts in abnormal contraction. Therefore, we investigated the relationship between lipid rafts and SPC-induced Ca2+-sensitization of VSM. In addition, high performance liquid chromatography (HPLC) and mass spectrometry (MS) revealed prophylactic compounds that can markedly inhibit the Ca2+-sensitization, without affecting physiological Ca2+-dependent contraction. These results suggest that innovative natural ingredient may contain novel protective components active against abnormal VSM contraction.
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