Analysis of skeletal muscle fiber type transition by fatty acids using isolated fiber culture system
| Project/Area Number |
22580136
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Kyushu University |
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Principal Investigator |
MIZUNOYA Wataru 九州大学, 大学院・農学研究院, 助教 (20404056)
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| Co-Investigator(Renkei-kenkyūsha) |
IKEUCHI Yoshihide 九州大学, 大学院・農学研究院, 教授 (90168112)
TATSUMI Ryuichi 九州大学, 大学院・農学研究院, 准教授 (40250493)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 栄養化学 / 骨格筋 / 筋線維型 / 脂肪酸 / 筋線維タイプ / ラット / PPARδ / ミオシン重鎖 / 単離筋線維 / エイコサペンタエン酸 / PPARσ / 衛星細胞 |
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| Research Abstract |
Skeletal muscle fibers are classified as slow-type and fast-type based on color, metabolism and contractile properties. Here, we tested whether food component could affect muscle fiber type. Here, we succeeded in establishing a rat muscle fiber-culture assay, in which fibersexpress mature myofibrillar proteins. Then, we revealed that PDK4 and UCP3 mRNAs, known target genes of PPARδand abundant in slow-type fibers, were up-regulated when eicosapentaenoic acid (EPA), abundant in fish oil, was added to fiber cultures for 24 hours.
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Report
(4 results)
Research Products
(52 results)
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[Journal Article] Calcium influx through a possible coupling of cation channels impacts skeletal muscle satellite cell activation in response to mechanical stretch2012
Author(s)
Hara M., Tabata K., Suzuki T., Do M.K., Mizunoya W., Nakamura M., Nishimura S., Tabata S., Ikeuchi Y., Sunagawa K., Anderson J.E., Allen R.E. and Tatsumi R
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Journal Title
Am J Physiol Cell Physiol
Volume: 302
Related Report
Peer Reviewed
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[Journal Article] Growth factor regulation of neural chemorepellent Sema3A expression in satellite cell cultures2011
Author(s)
Do, M.-K. Q., Sato, Y., Shimizu, N., Suzuki, T., Shono, J.-I., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J. E., and Tatsumi, R
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Journal Title
American Journal of Physiology-Cell Physiology
Volume: 301
Issue: 5
Pages: C1270-C1279
DOI
Related Report
Peer Reviewed
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[Presentation] Eicosapentaenoic acid affects skeletal muscle fiber types relating factors via PPARo pathway in isolated fibers of rats., Keystone Symposia, Nuclear Receptors and Friends2013
Author(s)
Mizunoya W., Komiya Y., Anderson J.E., Goto T., Takahashi N., Kawada T., Nakamura M., Tatsumi R.and Ikeuchi Y
Organizer
Roles in Energy Homeostasis and Metabolic Dysfunction
Place of Presentation
Alpbach, Austria
Year and Date
2013-04-06
Related Report
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