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High sensitive LC-MS/MS analysis of acylated anthocyanins for determination in bioanalytical samples

Research Project

Project/Area Number 22580147
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionNihon University

Principal Investigator

MATSUFUJI Hiroshi  日本大学, 生物資源科学部, 准教授 (70287605)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords食品機能 / 分析科学 / アントシアニン / 安定性 / LC-MS分析
Research Abstract

It is well known that anthocyanins show excellent physiological functions. But it is unclear that anthocyanins play in living organisms, because they decompose easily in analysis, and there is no method to analyze anthocyanins high-sensitively in bioanalytical samples. Therefore, high sensitive LC-MS/MS analytical method of anthocyanins was developed. The limit of detection ranged from 0.05 to 1 pmol, resulted that the sensitivity was 100 times higher than those of HPLC. The recovery of anthocyanins from cell medium ranged from 70 to 140%. But the recovery from liver was0%, suggested the interference by ion suppression as phospholipid and so on.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (27 results)

All 2013 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (15 results) Remarks (3 results)

  • [Journal Article] β-carotene reverses the IL-1β-mediated reduction in paraoxanase-1 expression via induction of the CaMKKII pathway in human endotherial cells2012

    • Author(s)
      K. Yamagata, N. Tanaka, H. Matsufuji,M. Chino
    • Journal Title

      Microvascular Research

      Volume: 84(3) Issue: 3 Pages: 297-305

    • DOI

      10.1016/j.mvr.2012.06.007

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dietary apigenin regulates high glucose and hypoxic reoxygeneration-induced reductions in apelin expression in humanendothelial cells.2012

    • Author(s)
      K. Yamagata, C. Tagawa, H. Matsufuji, M. Chino
    • Journal Title

      Journal of Nutritional Biochemistry

      Volume: 23 Issue: 8 Pages: 929-936

    • DOI

      10.1016/j.jnutbio.2011.04.019

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dietary apigenin regulates high glucose and hypoxic reoxygeneration-induced reductions in apelin expression in human endothelial cells.2012

    • Author(s)
      K. Yamagata, C. Tagawa, H. Matsufuji, M. Chino
    • Journal Title

      Journal of Nutritional Biochemistry

      Volume: 23 Pages: 929-936

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] β-carotene reverses the IL-1β-mediated reduction in paraoxanase-1 expression via induction of the CaMKKII pathway in human endotherial cells2012

    • Author(s)
      K. Yamagata, N. Tanaka, H. Matsufuji, M. Chino
    • Journal Title

      Microvascular Research

      Volume: 84 Pages: 297-305

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ゴマ若葉に含まれるポリフェノール成分のラジカル消去活性2011

    • Author(s)
      松藤寛、大森潤一、後藤修一、千野誠、和田悦治、内田あゆみ、深堀勝謙、山形一雄、櫻井英敏
    • Journal Title

      日本食品科学工学会誌

      Volume: 58(3) Pages: 88-96

    • NAID

      10027868825

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Apigenin inhibits tumor necrosis alpha plus high glucose induced-LOX-1 expression in human endothelial cells2011

    • Author(s)
      K. Yamagata, A. Miyashita, M. Chino, H. Matsufuji
    • Journal Title

      Microvascular Research

      Volume: 81(1) Issue: 1 Pages: 60-67

    • DOI

      10.1016/j.mvr.2010.10.005

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ゴマ若葉に含まれるポリフェノール成分のラジカル消去活性2011

    • Author(s)
      松藤寛、大森潤一, 他7名
    • Journal Title

      日本食品科学工学会誌

      Volume: 58 Pages: 88-96

    • NAID

      10027868825

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 天然物酸化防止剤ローズマリー抽出物中の活性成分と活性寄与率2010

    • Author(s)
      松藤寛、千野誠、山形一雄、山崎壮
    • Journal Title

      日本食品化学学会誌

      Volume: 17(3) Pages: 164-170

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 天然酸化防止剤ローズマリー抽出物中の活性成分と活性寄与率2010

    • Author(s)
      松藤寛
    • Journal Title

      日本食品化学学会誌

      Volume: 17 Pages: 164-170

    • NAID

      110008661308

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] TNFaによるヒト血管内皮細胞の障害に対するGLP-1と食品成分の予防作用の比較2013

    • Author(s)
      謝雅潔、田中紀子、鈴木成美、鈴木祥子、千野誠、松藤寛、田上幹樹、山形一雄
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      神奈川県、パシフィコ横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] Free,conjugated, and halogenated estrogens in treated wastewater effluent2013

    • Author(s)
      D.R. Griffith, M.C. Kido Soule, H. Matsufuji, T. I. Eglinton, E.B. Kujawinski, P.M. Gschwend
    • Organizer
      ASLO2013 Aquatic Sciences Meeting
    • Place of Presentation
      New Orleans, Louisiana, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Free, Conjugated, and Halogenated Estrogens in Treated Wastewater Effluent2013

    • Author(s)
      D.R. Griffith, M.C. Kido Soule, H. Matsufuji, T. I. Eglinton, E.B. Kujawinski, P.M.Gschwend
    • Organizer
      ASLO 2013 Aquatic Sciences Meeting
    • Place of Presentation
      New Orleans, Louisiana
    • Related Report
      2012 Annual Research Report
  • [Presentation] ゴマ若葉及びスプラウト中の抗酸化ポリフェノールについて2012

    • Author(s)
      松藤寛、東果奈、鹿島なつみ、丸山文月、千野誠、和田悦治、内田あゆみ、深堀勝謙、山形一雄
    • Organizer
      日本食品科学工学会第59回大会
    • Place of Presentation
      札幌市、藤女子大学
    • Year and Date
      2012-08-31
    • Related Report
      2012 Final Research Report
  • [Presentation] アントシアニンの機能性と安定性-高感度分析のチャレンジと問題点-2011

    • Author(s)
      松藤寛
    • Organizer
      植物色素研究会23回集会
    • Place of Presentation
      都城市、南九州大学
    • Year and Date
      2011-11-19
    • Related Report
      2012 Final Research Report
  • [Presentation] アントシアニンの機能性と安定性-高感度分析のチャレンジと問題点-2011

    • Author(s)
      松藤寛
    • Organizer
      植物色素研究会第23回集会
    • Place of Presentation
      宮崎県都城市南九州大学
    • Year and Date
      2011-11-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] ゴマ若葉ポリフェノールの生育中の含量変化と熱安定性2011

    • Author(s)
      松藤寛、森智代、秋山達哉、和田悦治、内田あゆみ、深堀勝謙、千野誠、山形一雄
    • Organizer
      日本食品科学工学会第58回大会
    • Place of Presentation
      仙台市、東北大学
    • Year and Date
      2011-09-11
    • Related Report
      2012 Final Research Report
  • [Presentation] ゴマ若葉ポリフェノールの生育中の含量変化と熱安定性2011

    • Author(s)
      松藤寛、森智代, 他6名
    • Organizer
      日本食品科学工学会第58回大会
    • Place of Presentation
      仙台市東北大学
    • Year and Date
      2011-09-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 高濃度ローズマリー抽出物の加熱により生成する分解物と遺伝毒性2011

    • Author(s)
      松藤寛、丸山千明、高橋明日香、千野誠、山形一雄、山崎壮
    • Organizer
      日本食品化学学会第17回総会・学術大会
    • Place of Presentation
      東京ビックサイト
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] ヒト血管内皮細胞における食品成分のHDL様刺激作用2011

    • Author(s)
      田中紀子、松藤寛、千野誠、山形一雄
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡市、ツインメッセ静岡
    • Related Report
      2012 Final Research Report
  • [Presentation] Stability for extraction and analysis of acylated anthocyanins2010

    • Author(s)
      松藤寛
    • Organizer
      240^<th> American Chemical Society National Meeting
    • Place of Presentation
      米国ボストン市
    • Year and Date
      2010-08-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Stability for extraction and analysis of acylated anthocyanins2010

    • Author(s)
      H. Matsufuji
    • Organizer
      240th American Chemical Society National Meeting
    • Place of Presentation
      Boston, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] ゴマ若葉及びスプラウト中の抗酸化ポリフェノールについて

    • Author(s)
      松藤 寛、他8名
    • Organizer
      日本食品科学工学会第59回大会
    • Place of Presentation
      札幌市、藤女子大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] Isolation of syringaldehyde, low-molecular-weight lignin, transforming bacteria from Kamaishi bay after the occurring of the Great East Japan Earthquake

    • Author(s)
      H. Takiguchi, H. Matsufuji, 他4名
    • Organizer
      The 28th Annual Meeting of the Japanease Society of Microbial Ecology (JSME 2012, 第28回日本微生物生態学会大会)
    • Place of Presentation
      豊橋市、豊橋技術科学大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] TNFaによるヒト血管内皮細胞の障害に対するGLP-1と食品成分の予防作用の比較

    • Author(s)
      謝 雅潔、松藤 寛、他6名
    • Organizer
      日本薬学会第113年会
    • Place of Presentation
      横浜市、パシフィコ横浜
    • Related Report
      2012 Annual Research Report
  • [Remarks] http://hp.brs.nihon-u.ac.jp/~eisei/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://hp.brs.nihon-u.ac.jp/~eisei/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://hp.brs.nihon-u.ac.jp/~eisei/group-matsufuji1.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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