| Project/Area Number |
22580168
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Forest science
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| Research Institution | Forestry and Forest Products Research Institute |
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Principal Investigator |
TANIGUCHI Toru 独立行政法人森林総合研究所, 森林バイオ研究センター, 室長 (00360470)
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| Co-Investigator(Kenkyū-buntansha) |
KONAGAY Ken-ichi 森林総合研究所, 森林バイオ研究センター, 主任研究員 (30582762)
KURITA Manabu 森林総合研究所, 林木育種センター, 室長 (40370829)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 育種 / 遺伝子組換え / 雌性不稔化 / 遺伝子解析 / スギ / 雌性器官 / 発現遺伝子 / スギ雌花 / 胚珠 / クライオマイクロダイセクション / SSH / cDNAシーケンス / 次世代シーケンサー / レーザーマイクロダイセクション / RNA |
|---|
| Research Abstract |
Genetic engineering is a powerful tool for tree breeding with achievement of large genetic gain in short time. However, dispersal of transgenes by pollen or seeds should be prevented. In this study, expression genes in Cryptomeria japonica female reproductive organ was profiled through steps as follows: micro-dissection of micro tissues, ovule, and RNA extraction from the tissue; next generation sequence of ovule- specific suppression subtractive hybridization library; identifications of the ovule specific genes. Our results will be used for female sterilization of no-pollen C. japonica that have been produced by genetic engineering.
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