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Development of a novel system for the production of glucuronic acid by using glucuronan degrading enzymes

Research Project

Project/Area Number 22580179
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Wood science
Research InstitutionUtsunomiya University

Principal Investigator

HABU Naoto  宇都宮大学, 農学部, 教授 (10292575)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsグルクロナン / 酵素 / 微生物 / グルクロン酸 / TEMPO触媒酸化 / カードラン / カーデュロン酸 / カーデゥロン酸 / TEMPO酸化
Research Abstract

A novel system for the production of glucuronic acid by using the combination of TEMPO-mediated oxidation of glucans, such as starch and curdlan, and glucuronan degrading enzymes was investigated. A bacterial strain, having the ability to degrade (1→3)-β-polyglucuronate was isolated from soil, and identified as Paenibacillus sp. Hydrolytic enzyme activity, which was highly specific to (1→3)-β-polyglucuronate, was detected in the cell-free extract.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (6 results)

All 2013 2012 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results)

  • [Journal Article] Biodegradation of (1→3)-β-polyglucuronate prepared by TEMPO-mediated oxidation2013

    • Author(s)
      Erika Watanabe, Naoto Habu, Akira Isogai
    • Journal Title

      Carbohydrate Polymers

      Volume: 96 Pages: 314-319

    • URL

      http://dx.doi.org/10.1016/j.carbpol.2013.03.081

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biodegradation of (1→3)-beta-polyglucuronate prepared by TEMPO-mediated oxidation2013

    • Author(s)
      Watanabe, E.; Habu, N.; Isogai A.
    • Journal Title

      Carbohydrate Polymers

      Volume: 96 Issue: 1 Pages: 314-319

    • DOI

      10.1016/j.carbpol.2013.03.081

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] β-1,3-ポリグルクロン酸の化学安定性解析2012

    • Author(s)
      渡邉絵里香,磯貝明,羽生直人,田村直之,藤澤秀次
    • Organizer
      セルロース学会第19回年次大会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Final Research Report
  • [Presentation] β-1,3-ポリグルクロン酸の化学安定性解析2012

    • Author(s)
      渡邉絵里香,、磯貝 明,羽生直人,田村直之,藤澤秀次
    • Organizer
      セルロース学会第19回年次大会
    • Place of Presentation
      長野
    • Related Report
      2012 Annual Research Report
  • [Presentation] カージュロン酸分解菌の単離とその分解酵素の性質2011

    • Author(s)
      渡邉絵里香, 磯貝明, 羽生直人
    • Organizer
      セルロース学会第18回年次大会
    • Place of Presentation
      信州大学工学部
    • Year and Date
      2011-07-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] カージュロン酸分解菌の単離とその分解酵素の性質2011

    • Author(s)
      渡邉絵里香,磯貝明,羽生直人
    • Organizer
      セルロース学会第18回年次大会
    • Place of Presentation
      長野
    • Related Report
      2012 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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