Distribution of lignin precursors in tree cells
| Project/Area Number |
22580183
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Wood science
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
YOSHINAGA Arata 京都大学, 大学院・農学研究科, 助教 (60273489)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAMITAKAHARA Hiroshi 京都大学, 大学院・農学研究科, 准教授 (10293911)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
|---|
| Keywords | リグニン / 前駆物質 / コニフェリン / スクロース / 木質化 / 細胞壁 / MALDI-TOF MS / イメージング / MALDI TOF MS / 樹木細胞 / 顕微ラマン分光 / シリンジン / ヒノキ |
|---|
| Research Abstract |
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) was applied to detect monolignol glucosides in differentiating xylems of several Japanese softwoods. MALI-IMS combined with osmium tetroxide vapor treatment enabled us to distinguish distribution of sucrose and coniferin with the same molecular weight. MALDI-IMS using transverse sections of normal wood in Japanese cypress (Chamaecyparis obtusa) showed that coniferin was mainly distributed in tracheids during secondary wall formation.
|
Report
(4 results)
Research Products
(5 results)
