Digestive physiology to development the artificial diet for sea urchin.
Project/Area Number |
22580194
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General fisheries
|
Research Institution | Hokkaido University |
Principal Investigator |
URA Kazuhiro 北海道大学, 大学院・水産科学研究院, 助教 (90360940)
|
Co-Investigator(Renkei-kenkyūsha) |
TAKAGI Yasuaki 北海道大学, 大学院・水産科学研究院, 教授 (10212002)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | ウニ / 消化酵素 / 精製 / 特異抗体 / 局在 / 内臓 / 免疫組織 / 消化吸収 / 人工餌料開発 / プロテアアーゼ / 棘皮動物 / セルラーゼ |
Research Abstract |
Subtilase and cellulase exhibited hydrolytic activity toward carboxymethyl cellulose with optimum temperature and pH at 30 °C and pH 8.0, respectively. The thermal stability was characterized and temperature at which activity decreased to50% by the 30 min treatment at pH 7.0 was 32 °C. The isolated cellulase activity was remained at high level at 5-20 °C which is growth temperature of the short-spined sea urchin. Thus, we confirmed that a cellulase of the short-spined sea urchin is adaptiveenzyme around low water temperature.
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Report
(4 results)
Research Products
(5 results)