| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
Subtilase and cellulase exhibited hydrolytic activity toward carboxymethyl cellulose with optimum temperature and pH at 30 °C and pH 8.0, respectively. The thermal stability was characterized and temperature at which activity decreased to50% by the 30 min treatment at pH 7.0 was 32 °C. The isolated cellulase activity was remained at high level at 5-20 °C which is growth temperature of the short-spined sea urchin. Thus, we confirmed that a cellulase of the short-spined sea urchin is adaptiveenzyme around low water temperature.
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