Development of genetic breeding technology for double disease resistant strains

• Project/Area Number: 22580196
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: SAKAMOTO Takashi 東京海洋大学, 海洋科学技術研究科, 准教授 (40313390)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: アユ / 耐病性 / 連鎖解析 / マッピング / マーカー選抜育種 / MAS / 育種 / 魚類 / QTL 解析 / ビブリオ病 / マイクロサテライト / 遺伝子地図 / ゲノム

Research Abstract

We performed QTL analysis for disease resistant trait against Vibrio anguillarum (PH-0301) in Ayu (Plecoglossus altivelis) using microsatellite DNA markers. We identified the locus associated with the disease resistant trait on linkage group (LG) 18 in this species (LOD score：3.12, p < 0.001). On LG18, a microsatellite marker explained 14 % of the total phenotypic variation in the 94 individuals screened.

Research Products

• [Journal Article] Isolation and characterization of 10 polymorphic microsatellite loci in the devil stinger, Inimicus japonicas. (2013)
  • Author(s): T. Sakamoto, Y. Pan, E. Koshimizu, N.Okamoto, T. Nagai and R. Fuseya
  • Journal Title: Molecular Ecology Resources. Volume: 13(1) Issue: 1 Pages: 158-159
  • DOI: 10.1111/1755-0998.12035
  • Peer Reviewed
• [Journal Article] A Y-linked anti-Mullerian hormone duplication takes over a critical role in sex determination (2012)
  • Author(s): R.S. Hattori, Y. Murai, M. Oura, S. Masuda, S.K. Majhi, T. Sakamoto, J.I. Fernandino, G.M. Somoza, M. Yokota, and C.A. Strussmann. 2012.
  • Journal Title: Proceedings of the National Academy of Sciences USA Volume: 109 Issue: 8 Pages: 2955-2959
  • DOI: 10.1073/pnas.1018392109
  • Peer Reviewed
• [Journal Article] Isolation and characterization of polymorphic microsatellites from the mud crab (Scylla serrata) (2012)
  • Author(s): R. Fuseya, T. Sakamoto and S.Watanabe
  • Journal Title: Molecular Ecology Resources Volume: 11(6) Issue: 6 Pages: 1124-1126
  • DOI: 10.1111/j.1755-0998.2011.03068.x
  • Peer Reviewed
• [Journal Article] Non-sex specific genes associated with the secondary mitotic period of primordial germ cell proliferation in the gonads of embryonic rainbow trout (Oncorhynchus mykiss) (2011)
  • Author(s): J.J. Nagler, G. Yoshizaki(他5名)
  • Journal Title: Mol. Reprod. Dev Volume: 78巻 Issue: 3 Pages: 181-187
  • DOI: 10.1002/mrd.21277
  • Peer Reviewed
• [Journal Article] Linkage mapping of Toll-like receptors (TLRs) in Japanese flounder, Paralichthys olivaceus (2011)
  • Author(s): S. D. Hwang, K. Fuji, T. Takano, T.Sakamoto, H. Kondo, I. Hirono and T. Aoki
  • Journal Title: Marine Biotechnology Volume: 13(6) Issue: 6 Pages: 1086-1091
  • DOI: 10.1007/s10126-011-9371-x
  • Peer Reviewed
• [Journal Article] Non-sex specific genes associated with the secondary mitotic period of primordial germ cell proliferation in the gonads of embryonic rainbow trout (Oncorhynchus mykiss) (2011)
  • Author(s): J.J.Nagler, et al.
  • Journal Title: Molecular Reproduction and Development Volume: 78 Pages: 181-187
  • Peer Reviewed
• [Journal Article] Linkage mapping of Toll-like receptors (TLRs)in Japanese flounder. Paralichthys olivaceus (2011)
  • Author(s): S.D.Hwang, et al.
  • Journal Title: Marine Biotechnology Volume: 13(6) Pages: 1086-1091
  • Peer Reviewed
• [Journal Article] Construction of a genetic map and development of DNA markers linked to the sex determining locus in the Patagonian pejerrey (Odontesthes hatcheri) (2010)
  • Author(s): E. Koshimizu, C.A. Strussmann, N. Okamoto, H. Fukuda, and T. Sakamoto
  • Journal Title: Marine Biotechnology Volume: 12 Issue: 1 Pages: 8-13
  • DOI: 10.1007/s10126-009-9194-1
  • Peer Reviewed
• [Journal Article] Establishment of a strain inheriting a sex-linked SNP marker in Patagonianpejerrey (Odontesthes hatcheri), a species with both genotypic and temperature- dependent sex determination (2010)
  • Author(s): R.S. Hattori, M. Oura, T. Sakamoto, M.Yokota, S. Watanabe, and C.A. Strussmann
  • Journal Title: Animal Genetics Volume: 41 Issue: 1 Pages: 81-84
  • DOI: 10.1111/j.1365-2052.2009.01948.x
  • Peer Reviewed
• [Journal Article] Hyper-expansion of large DNA segments in the genome of kuruma shrimp, Marsupenaeus japonicus (2010)
  • Author(s): Koyama T, Asakawa S, Katagiri T, Shimizu A, Fagutao FF, Mavichak R, Santos MD, Fuji K, Sakamoto T, Kitakado T, Kondo H, Shimizu N, Aoki T, Hirono I
  • Journal Title: BMC Genomics Volume: 11 Issue: 1 Pages: 141-141
  • DOI: 10.1186/1471-2164-11-141
  • Peer Reviewed
• [Journal Article] Interspecific hybridization in the haploid blade-forming marine cropPorphyra (Bangiales Rhodophyta)occurrence of allodiploid in the survival F1 gametophytic blades (2010)
  • Author(s): K. Niwa, A. Kobiyama and T. Sakamoto
  • Journal Title: Journal of Phycology Volume: 46 Issue: 4 Pages: 693-702
  • DOI: 10.1111/j.1529-8817.2010.00853.x
  • Peer Reviewed
• [Journal Article] Allopolyploidy in natural and cultivated populations of Porphyra (Bangiales, Rhodophyta) (2010)
  • Author(s): K. Niwa and T. Sakamoto
  • Journal Title: Journal of Phycology Volume: 46 Issue: 6 Pages: 1097-1105
  • DOI: 10.1111/j.1529-8817.2010.00897.x
  • Peer Reviewed
• [Journal Article] Linkage analysis of resistance to Streptococcus iniae infection in Japanese flounder Paralichthys olivaceus) (2010)
  • Author(s): A. Ozaki, H. Okamoto, T. Yamada, T.Matuyama, T. Sakai, K. Fuji, T. Sakamoto, N. Okamoto, K. Yoshida, K.Hatori, K. Araki and M. Okauchi
  • Journal Title: Aquaculture Volume: 308 Pages: S62-S67
  • DOI: 10.1016/j.aquaculture.2010.07.039
  • Peer Reviewed
• [Journal Article] Identification of the sex-linked locus in yellowtail, Seriola quinqueradiata (2010)
  • Author(s): K. Fuji, K. Yoshida, K. Hattori, A.Ozaki, K. Araki, M. Okauchi, S. Kubota, N. Okamoto and T. Sakamoto
  • Journal Title: Aquaculture Volume: 308 Pages: S51-S55
  • DOI: 10.1016/j.aquaculture.2010.06.035
  • Peer Reviewed
• [Journal Article] A second generation genetic linkage map of Japanese flounder (Paralichthys olivaceus) (2010)
  • Author(s): C. C. Sanchez, K. Fuji, A. Ozaki, O.Hasegawa, T. Sakamoto, K. Morishima, I. Nakayama, A. Fujiwara, T. Masaoka, H. Okamoto, K. Hayashida, M. Tagami, J. Kawai, Y. Hayashizaki and N.Okamoto
  • Journal Title: BMC Genomics Volume: 11 Issue: 1 Pages: 554-554
  • DOI: 10.1186/1471-2164-11-554
  • Peer Reviewed
• [Journal Article] Establishment of a strain inheriting a sex-linked SNP marker in Patagonian pejerrey (Odontesthes hatcheri), a species with both genotypic and temperature-dependent sex determination. (2010)
  • Author(s): R.S.Hattori, etal.
  • Journal Title: Animal Genetics Volume: 41(1) Pages: 81-84
  • Peer Reviewed
• [Journal Article] Construction of a Genetic Map and Development of DNA Markers Linked to the Sex Determining Locus in the Patagonian Pejerrey (Odontesthes hatcheri). (2010)
  • Author(s): E.Koshimizu, etal.
  • Journal Title: Marine Biotechnology Volume: 12(1) Pages: 8-13
  • Peer Reviewed
• [Journal Article] A second generation genetic linkage map of Japanese flounder (Paralichthys olivaceus). (2010)
  • Author(s): C.C.Sanchez, etal.
  • Journal Title: BMC Genomics Volume: 11 Pages: 554-554
  • Peer Reviewed
• [Journal Article] Identification of the sex-linked locus in yellowtail, Seriola quinqueradiata. (2010)
  • Author(s): K.Fuji, etal.
  • Journal Title: Aquaculture Volume: 308, Supplement 1 Pages: 51-55
  • Peer Reviewed
• [Journal Article] Linkage analysis of resistance to Streptococcus iniae infection in Japanese flounder (Paralichthys olivaceus). (2010)
  • Author(s): A.Ozaki, etal.
  • Journal Title: Aquaculture Volume: 308, Supplement I Pages: 62-67
  • Peer Reviewed
• [Presentation] 養殖魚類における耐病性形質のマーカーアシスト選抜 (2012)
  • Author(s): 坂本 崇
  • Organizer: 平成23年度日本水産学会秋季大会シンポジウム
  • Place of Presentation: 長崎大学(長崎市)
  • Year and Date: 2012-09-28
• [Presentation] Marker-Assisted Selection in Aquaculture: Strategies and Applications (2012)
  • Author(s): Takashi Sakamoto
  • Organizer: In "Genetic Improvement of Livestock and Aquatic Animals in the Tropics: Challenge and Reward"(GILAAT 2012)
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Marker-Assisted Selection in Aquaculture: Strategies and Applications (2012)
  • Author(s): Takashi Sakamoto
  • Organizer: “Genetic Improvement of Livestock and Aquatic Animals in the Tropics: Challenge and Reward" (GILAAT 2012)
  • Place of Presentation: Bangkok, Thailand
  • Invited
• [Presentation] 養殖魚類における耐病性形質のマーカーアシスト選抜 (2011)
  • Author(s): 坂本崇
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎大学(長崎市)(招待講演)
  • Year and Date: 2011-09-28
• [Presentation] マーカーアシスト選抜育種法によるアユ冷水病耐性および感受性系統の作出 (2011)
  • Author(s): 大島晴高, 他6名
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東北・関東大震災のため学会が中止となり要旨集の発行をもって発表となった
  • Year and Date: 2011-03-27
• [Presentation] HiCEP法によるアユ冷水病耐性形質関連遺伝子の単離 (2011)
  • Author(s): 中島哲郎, 他7名
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東北・関東大震災のため学会が中止となり要旨集の発行をもって発表となった
  • Year and Date: 2011-03-27
• [Presentation] マーカーアシスト選抜育種法によるアユ冷水病耐性および感受性系統の作出 (2011)
  • Author(s): 大島晴高・森本昌平・永井崇裕・川口 修・飯田悦左・村上啓士・坂本 崇
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都)
• [Presentation] HiCEP法によるアユ冷水病耐性形質関連遺伝子の単離 (2011)
  • Author(s): 中島哲郎・森本昌平・大島晴高・永井崇裕・川口修・飯田悦左・村上啓士・坂本崇
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都)
• [Presentation] アユMHCクラスI・クラスII遺伝子のマッピング (2010)
  • Author(s): 松平彩花・輿水江里子・坂本崇
  • Organizer: 第11回日本動物遺伝育種学会
  • Place of Presentation: 白河市(福島県)
  • Year and Date: 2010-10-06