The verification of the alternative usage hypothesis of the neuro-motor system in teleosts. An attempt in the ayu (Plecoglossus altivelis altivelis) as a model fish by using a molecular marker c-fos.

• Project/Area Number: 22580198
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hiroshima University
• Principal Investigator: UEMATSU Kazumasa 広島大学, 大学院・生物圏科学研究科, 教授 (00116542)
• Co-Investigator(Kenkyū-buntansha): FUJIKAWA Yukichi 広島大学, 大学院・生物圏科学研究科, 講師 (10506687)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: アユ / 脳 / 産卵中枢 / 裸状型卵巣 / 体側筋 / c-fos / 最初期遺伝子 / 特異抗体 / 遺伝子発現 / タンパク質発現 / 抗体作製 / 脳地図 / クローニング / カイニン酸

Research Abstract

The salmonids and the ayu Plecoglossus altivelis altivelis use the trunk musculature also for spawning, but concerned brain regions have been unknown.In order to obtain a specific molecular tool to determine brain regions regulating spasmodic body contraction in the spawning ayu, we firstly isolated c-fos cDNA from the ayu brain. Based on the sequence, we obtained the full length recombinant ayu c-Fos protein and specific antisera by using the protein as antigens.Immunostaining of brain sections of the spawned ayu with the antiserum suggested that the concerned brain area might correspond to the midbrain locomotor region.