Establishment of the maturity control technique aimingat advance of the fish aquaculture techniques

• Project/Area Number: 22580199
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Ehime University
• Principal Investigator: MIURA Chiemi 愛媛大学, 南予水産研究センター, 講師 (90518002)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 成長 / 成熟 / 魚類 / 性成熟 / 配偶子形成 / 成長ホルモン / インスリン様成長因子 / 生殖腺培養系 / 性ステロイドホルモン / 性成熟配偶子形成

Research Abstract

Growth initiates sexual maturation in fish. However, the onset of maturity leads to a marked loss of body weight. To avoid therisks associated with of spawning, yellowtail farmers have tried to restrict the dietfrom winter to spring, a period just before the breeding season. By restricting the diet they can avoid the reduction in body weight. In this study, we show how growth of fish is dependent on the control of diet by using endocrinological and physiological analysis of these cultured yellowtails.

Research Products

• [Journal Article] Proliferating spermatogonia are susceptible to reactive oxygen species attack in japanese eel (Anguilla japonica). (2012)
  • Author(s): Celino, F. T., Shimizu-Yamaguchi, S., Miura, C. and Miura, T.
  • Journal Title: Biol. Reprod Volume: 87(3):70 Pages: 1-9
• [Journal Article] Taurine plays an important role in the protection of spermatogonia from oxidative stress. (2012)
  • Author(s): Higuchi, M., Celino, F. T., Shimizu-Yamaguchi, S., Miura, C. and Miura, T.
  • Journal Title: Amino Acids Volume: 43 Pages: 2359-2369
• [Journal Article] Gonads directly regulate growth in teleosts. (2012)
  • Author(s): Bhatta, S., Iwai, T., Miura, C., Higuchi, M., Shimizu-Yamaguchi, S., Fukada, H., Miura, T.
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 109 Pages: 11408-11412
• [Journal Article] The synthesis and role of taurine in the Japanese eel testis. (2012)
  • Author(s): Higuchi, M., Celino, F. T., Tamai, A., Miura, C. and Miura, T.
  • Journal Title: Amino Acids Volume: 43 Pages: 773-781
• [Journal Article] The Synthesis and Role of Taurine in the Eel Spermatogenesis. (2012)
  • Author(s): Higuchi, M., Celino. F. T., Miura, C. and Miura, T.
  • Journal Title: Interdisciplinary Studies on Environmental Chemistry-Environmental Pollution and Ecotoxicology Pages: 35-40
• [Journal Article] Molecular characterization and gene expression of Japanese eel (Anguilla japonica) gonadotropin receptors. (2012)
  • Author(s): Kazeto, Y., Koyama, M., Tosaka, R., Gen, K., Yokoyama, M., Miura, C., Miura, T., Adachi, S. and Yamauchi, K.
  • Journal Title: Zoological. Science Volume: 29(3) Pages: 204-211
  • NAID: 40019178839
• [Journal Article] Differences between male and female growth and sexual maturation in tilapia (Oreochromis mossanbicus). (2012)
  • Author(s): Bhatta, S., Iwai, T., Miura, T., Higuchi, M., Gersende, M. and Miura, C.
  • Journal Title: KUSET Volume: 8 Issue: 2 Pages: 57-65
  • DOI: 10.3126/kuset.v8i2.7326
  • Peer Reviewed
• [Journal Article] Molecular characterization and gene expression of Japanese eel (Anguilla japonica) gonadotropin receptors (2012)
  • Author(s): Yokinori Kazeto, et al
  • Journal Title: Zoological Science Volume: (in press)
  • Peer Reviewed
• [Journal Article] The role of taurine in the eel spermatogenesis (2012)
  • Author(s): Masato Higuchi, et al
  • Journal Title: Interdisciplinary Studies on Environmental Chemistry Amino Acids Volume: 6 Pages: 35-40
  • Peer Reviewed
• [Journal Article] The synthesis and role of taurine in the Japanese eel testis (2012)
  • Author(s): Masato Higuchi, et al
  • Journal Title: Amino Acids Volume: (in press)
  • Peer Reviewed
• [Journal Article] Tolerance of spermatogonia to oxidative stress is due to high levels of Zn and Cu/Zn superoxide dismutase. (2011)
  • Author(s): Celino F.T., Yamaguchi, S., Miura, C., Ohta, T., Tozawa, Y., Iwai, T. and Miura, T.
  • Journal Title: Plos One Volume: 6(2)
• [Journal Article] Differences of two polychaete species reflected in enzyme activities. (2011)
  • Author(s): Ito, K., Nozaki, M., Ohta, T., Miura, C., Tozawa, Y. and Miura, T.
  • Journal Title: Marine Biology Volume: 150 Pages: 1211-1221
• [Journal Article] Anti-viral effects of interferon administration on seven-band grouper (2011)
  • Author(s): Ohta, T., Ueda, Y. Ito, K., Miura, C., Yamashita, H., Miura, T. and Tozawa, Y.
  • Journal Title: Epinephelus septemfasciatus. Fish & Shellfish Immunology Volume: 30 Pages: 1064-1071
• [Journal Article] Analysis of spermatogenesis using an eel model. (2011)
  • Author(s): Miura, C., Miura, T.
  • Journal Title: Aqua-Bio Science Monographs Volume: 4 Pages: 105-129
• [Journal Article] Growth hormone is produced by the testis of Japanese eel and stimulates proliferation of spermatogonia. (2011)
  • Author(s): Miura, C., Shimizu, Y., Uehara, M., Ozaki, Y., Young, G. and Miura, T.
  • Journal Title: Reproduction Volume: 142 Pages: 869-877
• [Journal Article] マダイの成長と免疫系に及ぼすオニヒトデの効果 (2011)
  • Author(s): 三浦智恵美・三浦 猛
  • Journal Title: 養殖 Volume: 48 (2) Pages: 44-47
• [Journal Article] 技術-養殖ブリの産卵リスクとその制御のための給餌管理-制限給餌による成熟抑制と成長遅滞の解消- (2011)
  • Author(s): 三浦 猛・三浦智恵美・三浦仁嗣
  • Journal Title: アクアネット Volume: 14 (1) Pages: 52-59
• [Journal Article] Gh is produced by the testis of Japanese eel and stimulates proliferation of spermatogonia (2011)
  • Author(s): Chiemi Miura, et al
  • Journal Title: Reproduction Volume: 142 Pages: 869-877
  • Peer Reviewed
• [Journal Article] Analysis of spermatogenesis using an eel model (2011)
  • Author(s): Chiemi Miura, et al
  • Journal Title: Aqua-BioScience Monographs Volume: 4 Pages: 105-129
  • Peer Reviewed
• [Journal Article] Anti-viral effects of interferon administration on seven-band grouper, Epinephelus septemfasciatus (2011)
  • Author(s): Takashi Ohta, et al
  • Journal Title: Fish & Shellfish Immunology Volume: 30 Pages: 1064-1071
  • Peer Reviewed
• [Journal Article] Tolerance of spermatogonia to oxidative stress is due to high levels of Zn and Cu/Zn superoxide dismutase (2011)
  • Author(s): Fritzie T Celino, et al.
  • Journal Title: PLoS ONE|www.plosone.org Volume: 6(2)
  • Peer Reviewed
• [Journal Article] Differences of two polychaete species reflected in enzyme activities (2011)
  • Author(s): Katsutoshi Ito, et al.
  • Journal Title: Marine Biology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Study of sediment cleanup using polychaete. (2010)
  • Author(s): Ito, K., Nozaki, N., Kunihiro, T., Miura, C. and Miura, T.
  • Journal Title: International Symposium on Modeling and Analysis of Marine Environmental Problems(MAMEP Sympo. 2010) Pages: 133-139
• [Presentation] 魚類の生殖腺の機能1(生殖腺による成長制御) (2012)
  • Author(s): Bhatta, Sandip・岩井俊治・三浦智恵美・三浦猛
  • Organizer: 平成 24 年度日本動物学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] 魚類の生殖腺の機能2(異所性移植による配偶子形成) (2012)
  • Author(s): 岩井俊治・Bhatta, Sandip・三浦智恵美・三浦猛
  • Organizer: 平成 24 年度日本動物学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] トリプシンおよびタウリンによる精子形成での減数分裂制御機構. (2012)
  • Author(s): 樋口理人・三浦智恵美・三浦猛
  • Organizer: 平成 24 年度日本動物学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] Testicular growth hormone stimulates spermatogonial proliferation. (2012)
  • Author(s): Miura, C., Shimizu, Y., Young, G. and Miura, T.
  • Organizer: The 7th AOSCE Congress
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Year and Date: 2012-03-04
• [Presentation] Gonad is regulatory organ for growth in teleost. (2012)
  • Author(s): Miura,T., Iwai, T., Bhatta, S. and Miura, C..
  • Organizer: The 7thAOSCE Congress
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Year and Date: 2012-03-04
• [Presentation] Teaticular growth hormone stimulates spermatogonial proliferation (2012)
  • Author(s): Chiemi Miura
  • Organizer: the 7^<th> Asia and Oceania Society for Comparative Endocrinology (AOSCE) Congress
  • Place of Presentation: クアラルンプール、マレーシア
  • Year and Date: 2012-03-04
• [Presentation] Gonad is regulatory organ for growth in teleost (2012)
  • Author(s): Takeshi Miura, et al
  • Organizer: the 7^<th> Asia and Oceania Society for Comparative Endocrinology (AOSCE) Congress
  • Place of Presentation: クアラルンプール、マレーシア
  • Year and Date: 2012-03-04
• [Presentation] Growth hormone in synthesis in the testis and stimulates spermatogonial proliferation in Japanese eel (2011)
  • Author(s): Miura, C., Shimizu, Y., Uehara, M., Ozaki, Y., Young, G. and Miura, T.
  • Organizer: Anguilla japonica World Congress on Reproductive Biology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2011-10-11
• [Presentation] Gonad in one of the control organs for growth in fish (2011)
  • Author(s): Bhatta, S., Iwai, T., Miura, C., Higuchi, M., Yamaguchi, S. and Miura, T.
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2011-10-11
• [Presentation] Synthesis of taurine and its role in Japanese eel spermatogenesis. (2011)
  • Author(s): Higuchi, M., Miura, C. and Miura, T.
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2011-10-10
• [Presentation] Gonad in one of the control organs for growth in fish (2011)
  • Author(s): Sandip Bhatta, et al
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: ケアンズ、オーストラリア
  • Year and Date: 2011-10-10
• [Presentation] Growth hormone in synthesis in the testis and stimulates spermatogonial proliferation in Japanese eel, Anguilla japonica (2011)
  • Author(s): Chiemi Miura, et al
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: ケアンズ、オーストラリア
  • Year and Date: 2011-10-10
• [Presentation] Synthesis of taurine and its role in Japanese eel spermatogenesis (2011)
  • Author(s): Masato Higuchi, et al
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: ケアンズ、オーストラリア
  • Year and Date: 2011-10-10
• [Presentation] The induction of sterility using RNA interference technique in Pinctada fucata martensi (2011)
  • Author(s): Watanabe, N., Celino. F. T., Miura, C., Ohashi, H., Fukushima, E., Iwai, T., Hosokawa, H., Uchimura, Y. and Miura, T.
  • Organizer: The international Symposium on Pearl Research
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2011-10-05
• [Presentation] Selective bleeding of disease-resistant Japanese pearl oysters using molecular markers. (2011)
  • Author(s): Sone, K., Iwai, T., Uehara, M., Watanabe, N., Celino. F. T., Hosokawa, H., Odawara, K., Miura, C. and Miura, T.
  • Organizer: The international Symposium on Pearl Research
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2011-10-05
• [Presentation] Newly developed technique of producing pearl using mantle fragment of Pinctada margaritiferaand Pteria penguinin hyposensitized Akoya pearl oyster, Pinctada fucata. (2011)
  • Author(s): Fukushima, E., Iwai, T., Miura, C., Watanabe, N., Urasaki, S., Kuwayama, K., Odawara, K. and Miura, T.
  • Organizer: The international Symposium on Pearl Research
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2011-10-05
• [Presentation] 生殖腺は魚類の成長をコントロールする (2011)
  • Author(s): Sandip Bhatta・岩井俊治・三浦智恵美・*樋口理人・清水園子・三浦 猛
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎市
  • Year and Date: 2011-09-30
• [Presentation] ティラピアの成長の雌雄差と生殖腺の発達の関係について (2011)
  • Author(s): Sandip Bhatta・樋口理人・岩井俊治・清水園子・三浦智恵美・三浦猛
  • Organizer: 平成 23 年度日本水産学会秋季大会
  • Place of Presentation: 長崎市
  • Year and Date: 2011-09-29
• [Presentation] タウリンがウナギ精子形成の及ぼす作用1 (2011)
  • Author(s): 樋口理人・三浦智恵美・三浦 猛
  • Organizer: 平成 23 年度日本水産学会秋季大会
  • Place of Presentation: 長崎市
  • Year and Date: 2011-09-29
• [Presentation] タウリンがウナギ精子形成の及ぼす作用2 (2011)
  • Author(s): 樋口理人・三浦智恵美・三浦 猛
  • Organizer: 平成 23 年度日本水産学会秋季大会
  • Place of Presentation: 長崎市
  • Year and Date: 2011-09-29
• [Presentation] タウリンがウナギ精子形成の及ぼす作用3 (2011)
  • Author(s): 樋口理人・三浦智恵美・三浦 猛
  • Organizer: 平成 23 年度日本水産学会秋季大会
  • Place of Presentation: 長崎市
  • Year and Date: 2011-09-29
• [Presentation] Effect of taurine on eel spermatogenesis. (2011)
  • Author(s): Higuchi, M., Celino. F. T., Miura, C. and Miura, T.
  • Organizer: International symposium on Advanced Studies by Young Scientists on Environmental Pollution and Ecotoxicology
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 2011-08-05
• [Presentation] ニホンウナギ精巣でのタウリン合成機構 (2011)
  • Author(s): 樋口理人・Celino. F. T.・玉井綾子・山口園子・三浦智恵美・三浦 猛
  • Organizer: 平成 23年度日本水産学会春季大会
  • Place of Presentation: 東京都
  • Year and Date: 2011-03-30
• [Presentation] 魚類の成長を直接コントロールする器官としての生殖腺 (2011)
  • Author(s): Sandip Bhatta・岩井俊治・ 樋口理人・山口園子・三浦智恵美・三浦 猛
  • Organizer: 平成 23 年度日本水産学会春季大会
  • Place of Presentation: 東京都
  • Year and Date: 2011-03-28
• [Presentation] オニヒトデのマダイ成長と免疫系に及ぼす効果 (2010)
  • Author(s): 三浦智恵美・伊藤克敏・上田祐輔・太田加代子・岩井俊治・太田耕平・浦崎慎太郎・山本正文・三浦 猛
  • Organizer: 平成 22年度日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 2010-09-24
• [Presentation] 制限給餌による養殖ブリの成熟・成長コントロール (2010)
  • Author(s): 吉原勇作・三浦智恵美・山口園子・樋口理人・竹田 豊・浜田克人・三浦仁嗣・三浦 猛
  • Organizer: 平成 22 年度日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 2010-09-24
• [Presentation] イエバエ由来物の魚類成長および免疫系への作用. (2010)
  • Author(s): 岩井俊治・伊藤克敏・上田祐輔・三浦智恵美・浦崎慎太郎・太田加代子・山口園子・串間充崇・三浦 猛
  • Organizer: 平成22 年度日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 2010-09-24
• [Presentation] 酸化ストレスに対する魚類精原細胞の防御機構 (2010)
  • Author(s): 山口園子・Fritze T. Celino・三浦智恵美・三浦 猛
  • Organizer: 平成 22 年度日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 2010-09-24
• [Presentation] ゴカイ類が有する有機物分解能の分子生物学的解析 (2010)
  • Author(s): 伊藤克敏・野崎真奈・太田 史・戸澤 謙・三浦智恵美・三浦 猛
  • Organizer: 平成22 年度日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 2010-09-24
• [Presentation] マハタ組み換えインターフェロンの効果 (2010)
  • Author(s): 上田祐輔・太田 史・伊藤克敏・岩井俊治・浦崎慎太郎・山下浩史・戸澤 譲・三浦智恵美・三浦 猛
  • Organizer: 平成 22 年度日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 2010-09-24
• [Presentation] オニヒトデのマダイ成長と免疫系の及ぼす効果 (2010)
  • Author(s): 三浦智恵美, ら
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 2010-09-24
• [Presentation] 制限給餌による養殖ブリの成熟・成長コントロール (2010)
  • Author(s): 吉原勇作, ら
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 2010-09-24
• [Presentation] 酸化ストレスに対する魚類精原細胞の防御機構 (2010)
  • Author(s): 山口園子, ら
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 2010-09-24
• [Presentation] 魚類の生殖腺の機能１（生殖腺による成長制御）
  • Author(s): 三浦智恵美
  • Organizer: 日本水産学会
  • Place of Presentation: 大阪大学
• [Presentation] 魚類の生殖腺の機能２（異所性移植による配偶子形成）
  • Author(s): 岩井俊治
  • Organizer: 日本水産学会
  • Place of Presentation: 大阪大学
• [Patent(Industrial Property Rights)] 機能性養殖飼料及びその製造方法 (2010)
  • Inventor(s): 三浦猛, ら
  • Industrial Property Rights Holder: 愛媛大学
  • Industrial Property Number: 2010-220270
  • Filing Date: 2010-09-30