Development of techniques for seed production using new brain factors related to reproduction

• Project/Area Number: 22580204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: University of Miyazaki
• Principal Investigator: KAGAWA Hirohiko 宮崎大学, 農学部, 教授 (60169381)
• Co-Investigator(Renkei-kenkyūsha): UCHIDA Katsuhisa 宮崎大学, 農学部, 准教授 (50360508)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ウナギ / ハタ科魚類 / 人為催熟 / 種苗生産 / キスペプチン / キスペプチン受容体 / 成熟機構 / kisspeptin / GPR-54

Research Abstract

To develop new techniques for induction of sexual maturation using brain neuroendocrine factors related to reproduction, we newly isolated genes of kisspeptin and their receptors (GPR54) from brains of eels and honeycomb groupers and demonstrated that these genes play critical roles on the sexual maturation in the eel and the sex change in the grouper. Moreover, treatments of gonadotropin-releasing hormone analogue or of long photoperiod with high water temperature to sexually immature female grouper induced sexual maturation at period of non-breeding season.

Research Products

• [Journal Article] Using osmotic pumps to deliver hormones to induce sexual maturation of female Japanese eels (2013)
  • Author(s): H. Kagawa N. Fujie H. Imaizumi Y. Masuda Oda K, J. Adachi A. Nishi H. Hashimoto K. Teruya S. Kaji
  • Journal Title: Anguilla japonica. Aquaculture Volume: 388/391巻 Pages: 30-34
  • Peer Reviewed
• [Journal Article] Using osmotic pumps to deliver hormones to induce sexual maturation of female Japanese eels, Anguilla japonica (2013)
  • Author(s): Hirohiko Kagawa
  • Journal Title: Aquaculture Volume: 388/391 Pages: 30-34
  • DOI: 10.1016/j.aquaculture.2013.01.025
  • Peer Reviewed
• [Journal Article] Mechanism of oocyte maturation and ovulation, and its application to seed production in the Japanese eel. (2012)
  • Author(s): H. Kagawa Y. Sakurai Y. Kazeto K. Gen Im H. aizumi Y. Masuda
  • Journal Title: Fish Physiology and Biochemistry Volume: 39 Issue: 1 Pages: 13-17
  • DOI: 10.1007/s10695-012-9607-3
  • Peer Reviewed
• [Journal Article] Induction of sexual maturation of the female honeycomb grouper, Epinephelus merra, in the nonbreeding season by modulating environmental factors with GnRH analogue implantation (2012)
  • Author(s): T. Kanemaru M. Nakamura R. Murata K. Kuroki H. Horie K. Uchida B. Senthilkumaran H. Kagawa
  • Journal Title: Aquaculture Volume: 358/359巻 Pages: 85-91
  • Peer Reviewed
• [Journal Article] Induction of sexual maturation of the female honeycomb grouper, Epinephelus merra, in the non-breeding season by modulating environmental factors with GnRH analogue implantation. (2012)
  • Author(s): T. Kanemaru, M. Nakamura, R. Murata, K. Kuroki, H. Horie, K. Uchida, B. Senthilkumaran, H. Kagawa
  • Journal Title: Aquaculture Volume: 358-359 Pages: 85-91
  • DOI: 10.1016/j.aquaculture.2012.06.026
  • Peer Reviewed
• [Journal Article] 20β-hydroxysteroid dehydrogenase gene promoter: Potential role for cyclic AMP and xenobiotic responsive elements (2012)
  • Author(s): Gunti Sreenivasulu
  • Journal Title: Gene Volume: 509 Issue: 1 Pages: 68-76
  • DOI: 10.1016/j.gene.2012.07.017
  • Peer Reviewed
• [Journal Article] Expression and localization of aquaporin 1b during oocyte development in the Japanese eel (Anguilla japonica). (2011)
  • Author(s): H. Kagawa T. Kishi K. Gen Y. Kazeto ka.R, Tosa H. Matsubara T. Matsubara S. Sawaguchi
  • Journal Title: Reproductive Biology and Endocrinology Volume: 9 Issue: 1 Pages: 71-71
  • DOI: 10.1186/1477-7827-9-71
  • Peer Reviewed
• [Journal Article] Production of recombinant Japanese eel gonadotropins by baculovirus in silkworm larvae (2010)
  • Author(s): Kobayashi M, Hayakawa Y, Park W, Banba A, Yoshizaki G, Kumamaru K, Kagawa H, Kaki H, Nagaya H, Sohn YC
  • Journal Title: General and Comparative Endocrinology Volume: 167 Pages: 379-386
  • Peer Reviewed
• [Journal Article] Cloning, expression and enzyme activity analysis of testicular 11B-hydroxysteroid dehydrogenase during seasonal cycle and after hCG induction in air-breathing catfish Clarias gariepinus (2010)
  • Author(s): M.K.Rasheeda, H.Kagawa, R.Kirubagaran, A.Dutta-Gupta, B.Senthilkumaran
  • Journal Title: Journal of Steroid Biochemistry & Molecular Biology Volume: 120 Pages: 1-10
  • Peer Reviewed
• [Presentation] ウナギの発生・成長に伴う脳内キスペプチン様ペプチドの分布に関する免疫組織学的研究 (2013)
  • Author(s): 堀江ひかり、B. Senthilkumaran、今泉 均、増田賢嗣、神保忠雄、香川浩彦
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2013-03-29
• [Presentation] ウナギの発生・成長に伴う脳内kisspeptin様ペプチドの分布に関する免疫組織学的研究 (2013)
  • Author(s): 堀江ひかり
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
• [Presentation] ウナギの脳内におけるキスペプチン様ペプチドの分布に関する免疫組織学的研究 (2012)
  • Author(s): 堀江ひかり、B. Senthilkumaran、今泉均、増田賢嗣、神保忠雄、香川浩彦
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 独立行政法人水産大学校
  • Year and Date: 2012-09-15
• [Presentation] ウナギの脳内におけるkisspeptin様ペプチドの分布に関する免疫組織学的研究 (2012)
  • Author(s): 堀江ひかり
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 独立行政法人水産大学校
• [Presentation] Mechanism of oocyte maturation and ovulation, and its application to seed production in the Japanese eel (2011)
  • Author(s): H. Kagawa Y. Sakurai Y. Kazeto Gen, K, aizumi. Im H, Y. Masuda
  • Organizer: 9th International Symposium Reproductive Physiology of Fish
  • Place of Presentation: Cochin, India
  • Year and Date: 2011-08-10
• [Presentation] MECHANISM OF OOCYTE MATURATION AND OVULATION, AND ITS APPLICATION TO SEED PRODUCTION IN THE JAPANESE EEL (2011)
  • Author(s): Kagawa, H., Sakurai Y., Kazeto Y., Gen, K., Imaizumi, H., Masuda Y.
  • Organizer: 9th International Symposium Reproductive Biology of Fish
  • Place of Presentation: Cochin, India(招待講演)
  • Year and Date: 2011-08-10
• [Presentation] 非産卵期のカンモンハタEpinephelus merraの成熟は高水温および長日処置で誘導できる (2011)
  • Author(s): 金丸竜也, 村田良介, 中村將, 香川浩彦
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2011-03-27
• [Presentation] ウナギの卵成熟・排卵や卵質に及ぼすサケ脳下垂体抽出液および生殖腺刺激ホルモン放出ホルモンの効果 (2010)
  • Author(s): 堀内怜生、今泉均、増田賢嗣、橋本博、小田憲太朗、照屋和久、金丸竜也、増田洋亮、櫻井雄太、香川浩彦
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 京都大学
  • Year and Date: 2010-09-23
• [Presentation] 非産卵期カンモンハタEpinephelus merraの成熟に及ぼす環境因子調節及びGnRHa投与の効果 (2010)
  • Author(s): 金丸竜也、村田良介、中村将、Mohammad Alam、香川浩彦
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 京都大学
  • Year and Date: 2010-09-23
• [Book] うなぎ 謎の生物 (2012)
  • Author(s): 香川浩彦、他
  • Total Pages: 288
  • Publisher: 築地書館
• [Book] SPARIDAE Biology and aquaculture of gilthead sea bream and other species (2010)
  • Author(s): Mylonas, C.C., Zohar, Y., Pankhurst, N., Kagawa, H.
  • Publisher: Wiley-Blackwell